Hepatoprotective and antioxidant activity of Ipomoea staphylina Linn

Abstract

Background: Medicinal and aromatic plants have the potential to treat various disorders both human and animals. This present work investigated the hepatoprotective and antioxidant potential of aqueous extract of Ipomoea staphylina leaves on Wistar rats against CCl 4-induced liver injury. Methods: In vitro free radical scavenging activity of aqueous extract of Ipomoea staphylina (AIS) was estimated using ABTS [2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)} and DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radicals. Pharmacologically important phytochemicals such as phenolic compounds and flavonoids were analyzed using HPLC technique. Hepatotoxicity was induced by oral administration of CCl 4 at 1 mL/kg body weight of Wistar rats twice a week for 8 weeks. Experimental rats received AIS orally at the doses of 250 and 500 mg/kg daily for 8 weeks. Silymarin, a routine hepatoprotective drug, was used as a reference drug. Results: Total phenolics and flavonoid content of AIS are 20.78 ± 0.86 mg of GAE/g and 17.78 ± 0.45 mg of RE/g of extract respectively. ABTS and DPPH free radical scavenging activity of AIS exhibits the IC 50 value of 32.08 ± 0. 12 and 45.24 ± 0.65 μg/mL respectively. HPLC analysis of AIS showed the presence of polyphenolic compounds such as protocatechuic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, naringinin, qurecetin, rutin and flavon. Treatment with AIS restored the biochemical parameters towards normal level, prevented the occurrence of LPO and significantly (P ≤ 0.05) increased the levels of enzymatic (SOD and catalase) and non-enzymatic (reduced glutathione) antioxidants in CCl 4 administered Wistar rats. Histopathology results confirmed the hepatoprotective effect of AIS. Conclusion: Based on the results obtained, we suggest that the I. staphylina leaves extract have potent antioxidant and hepatoprotective activity.

Ameliorative effect of ethanolic extract of Limnophila rugosa (Scrophulariaceae) in paracetamol- and carbon tetrachloride-induced hepatotoxicity in rats

“…The liver taken from the killed animal was minced with a scalpel and homogenized in phosphate-buffered saline [30]. The tissue homogenate was centrifuged at 800 rpm for 10 min at 4 °C.…”

Actividad antioxidante e influencia del periodo climático sobre el contenido de polifenoles totales en Merremia aegyptia

“…Posteriormente, se indicó que en esta misma especie el contenido de polifenoles varía entre 6,3 y 13,5% (dependiendo del genotipo) [36], valores que distan de los hallados en el presente trabajo; no obstante, debe resaltarse que M. aegyptia crece abundantemente en suelos marginales del territorio guajiro, por lo que aun así representa un buen insumo para la producción de antioxidantes que merece ser estudiada a fondo con fines de aprovechamiento. Además, las propiedades medicinales de M. aegyptia y otras especies relacionadas han sido reportadas, destacándose su utilización como antimicrobiano, diurético, tratamiento de quemaduras en la piel y trastornos del hígado [2, 50,51], bioactividad que también ha sido asociada con la presencia de polifenoles.…”

Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H2O2-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency

“…Drug-induced liver injuries are also continue to increase as a major cause of liver disease ( Asrani et al, 2019 ). Many studies has been done to find antioxidant properties in medicinal plants that could be used as a potent candidate for liver injury medication ( Jeyadevi et al, 2019 , Aara et al, 2020 ). Red betel ( Piper crocatum Ruiz and Pav) is one of Indonesia’s medicinal plants that has been extensively used and known for its antioxidant activity ( Kusuma et al, 2017 , Lister et al, 2019a , Lister et al, 2019b ).…”