Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H2O2-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency

Lister, I Nyoman Ehrich; Ginting, Chrismis Novalinda; Girsang, Ermi; Nataya, Enden Dea; Azizah, Alya Mardhotillah; Widowati, Wahyu

doi:10.1016/j.jsps.2020.08.007

Cited by 23 publications

(17 citation statements)

References 54 publications

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“…The measurement was conducted according to Human IL-1β ELISA Kit (Elisa Max Deluxe, 437004; BioLegend, San Diego, CA, USA) and Human TNF-a ELISA Kit (Elisa Max Deluxe, 430204; BioLegend, San Diego, CA, USA) protocol. The treatments used in this experiments were as follows: (1) normal control; (2) BJ cells + DMSO 1% (vehicle control); (3) UV-induced BJ cells (positive control); (4) UV-induced BJ cells + CA 6.25 µg/ml; and (5) UV-induced BJ cells + CA 25 µg/ml (Laksmitawati et al, 2017;Widowati et al, 2016;Widowati et al, 2019a;Lister et al, 2020;Widowati et al, 2021).…”

Section: Measurement Of Il-1β and Tnf-amentioning

confidence: 99%

“…The plate was incubated for 5 min at room temperature. The result was read at 595 nm (Widowati et al, 2019a;Lister et al, 2020).…”

Section: Total Protein Measurementsmentioning

confidence: 99%

“…They were then stained with 25 µM DCFDA and incubated for 30 min in 37 C, with 5% CO 2 . The result was analyzed using MACSQuant Analyzer 10 Flow Cytometer (Miltenyi, Bergisch Gladbach, Germany) (Widowati et al, 2014;Niocel et al, 2019;Girsang et al, 2019c;Lister et al, 2020).…”

Section: Measurement Of Ros Levelmentioning

confidence: 99%

“…The pellet was added with 100 µl FACS buffer and stained by annexin and propidium iodide (PI). The stained cells were incubated for 1 h and analyzed using flow cytometry (Lister et al, 2020;Widowati et al, 2019b).…”

Section: Measurement Of Apoptotic Live Necrotic Cellsmentioning

confidence: 99%

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Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Girsang

Ginting

Lister

et al. 2021

PeerJ

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Background Skin aging is the most common dermatological problem caused by intrinsic and extrinsic factor, such as exposure to (ultraviolet) UV rays. Chlorogenic acid (CA) is a phenolic compound which is known for its antioxidant properties against oxidative stress. Objective This study investigates the antiaging and anti-inflammatory properties of CA on UV-induced skin fibroblast cells. Methods Anti-inflammatory properties of CA were assessed by measuring inflammatory-related proteins IL-1β and TNF-α, while antiaging properties of CA were assessed by measuring reactive oxygen species (ROS), apoptosis, live and necrotic cells, and COL-3 gene expression level. Results Treating UV-induced skin fibroblast cells with CA decreased the level of ROS, IL-1β, TNF-α, apoptotic cells, and necrotic cells and increased live cells and COL-3 gene expression. Conclusion CA has the potential as the protective compound against inflammation and aging by decreasing the level ROS, pro-inflammatory cytokines IL-1β and TNF-α, apoptotic cells, and necrotic cells and by increasing live cells and COL-3 gene expression.

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Section: Measurement Of Il-1β and Tnf-amentioning

confidence: 99%

“…The plate was incubated for 5 min at room temperature. The result was read at 595 nm (Widowati et al, 2019a;Lister et al, 2020).…”

Section: Total Protein Measurementsmentioning

confidence: 99%

Section: Measurement Of Ros Levelmentioning

confidence: 99%

Section: Measurement Of Apoptotic Live Necrotic Cellsmentioning

confidence: 99%

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Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Girsang

Ginting

Lister

et al. 2021

PeerJ

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“…Penelitian sebelumnya menjelaskan bahwa sirih merah mengandung metabolit sekunder berupa flavonoid, saponin, terpenoid, isoprenoid, eugenol, cyanogenic dan non protein asam amino. Flavonoid diketahui memiliki aktivitas sebagai antioksidan, antikanker, antiobesitas, dan antiinflamasi (Alfarabi et al, 2010;Lister et al, 2020;Rosa et al, 2020) .Namun informasi ilmiah terkait potensi sirih merah sebagai antiinflamasi pada penyakit Rheumatoid Arthritis (RA) masih sedikit dan umumnya belum banyak diketahui oleh masyarakat. Berdasarkan hal tersebut maka dilakukan penelitian lebih lanjut mengenai potensi daun sirih merah (Piper crocatum) untuk mengatasi penyakit Rheumatoid Arthritis yang dapat digunakan sebagai media informasi mengenai terapi Rheumatoid Arthritis (Lister et al, 2020).…”

Section: Pendahuluanunclassified

REVIEW: POTENTIAL OF RED BETEL LEAVES (Piper crocatum) AS ANTI-INFLAMMATORY IN Rheumatoid Arthritis

Aisyiyah¹,

Siregar²,

Kustiawan³

2021

JFSP

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Indonesia is a country with fertile soil and rich in plant species. Currently in society the use of medicinal plants as herbal treatment has increased. Elderly people often experience Rheumatoid Arthritis, which is inflammation of the synovial lining of the joints. One of the plants used by the community is red betel leaf (Piper crocatum). However, scientific information related to the potential of red betel as an anti-inflammatory in Rheumatoid Arthritis is still not widely known by the public. This study was conducted to explore the potential of P. crocatum leaves to treat Rheumatoid Arthritis which can be used as references regarding Rheumatoid Arthritis therapy. This study uses a narrative review method from previous studies that were collected related to the topic which was then discussed systematically. By using keywords in the search for Piper crocatum, anti-inflammatory activity, red betel bioactivity, secondary compounds in red betel leaf, and Rheumatoid Arthritis. P. crocatum leaves contain various bioactive compounds. Flavonoids have activity as primary and secondary antioxidants. Flavonoid compounds such as brazilien, brazilin, taxifolin, procyanidin found in red betel have anti-inflammatory activity in rheumatoid arthritis. Brazilin is one of the flavonoid compounds from P. crocatum leaves that has the potential as an anti-inflammatory in rheumatoid arthritis by regulating the transcription factor nuclear factor kappa B (NF-kB) for TNF- expression against inflammation.

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Recent insights into the hepatoprotective potential of medicinal plants and plant‐derived compounds

Thilagavathi

Begum

Varatharaj

et al. 2023

Phytotherapy Research

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Liver problems are a worldwide concern, and conventional medicinal therapies are ineffective. Hence, safeguarding the healthy liver is vital for good health and well‐being. Infections due to virus, immune problems, cancer, alcohol abuse, and an overdose of drugs are some of the causes of liver diseases. Antioxidants derived from medicinal plants and conventional dietary sources can protect the liver from damages caused by oxidative stress system and various chemicals. Plants and plant‐derived phytochemicals are appealing hepatoprotective agents since they have less side effects and still there is a lot of interest shown in using herbal tonics for treating liver disorders. This review therefore primarily focuses on newly discovered medicinal plants and compounds produced from plants that fall under the classifications of flavonoids, alkaloids, terpenoids, polyphenolics, sterols, anthocyanins, and saponin glycosides, all of which have the potential to be hepatoprotective. Hosta plantaginea, Ligusticum chuanxiong, Daniella oliveri, Garcinia mangostana, Solanum melongena, Vaccinium myrtillus, Picrorhiza kurroa, and Citrus medica are some potential plants having hepatoprotective effects. We conclude that these phytochemicals and the plant extracts listed above are used in the future to treat a variety of liver diseases, additional research is still needed to develop safer and more potent phytochemical drugs.

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Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H2O2-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency

Cited by 23 publications

References 54 publications

Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

REVIEW: POTENTIAL OF RED BETEL LEAVES (Piper crocatum) AS ANTI-INFLAMMATORY IN Rheumatoid Arthritis

Recent insights into the hepatoprotective potential of medicinal plants and plant‐derived compounds

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