Hepatic cholestasis during the post-embryonic development of fish larvae

Diaz, J. P.; Mani‐Ponset, Laurence; Guyot, E.; Connes, R.

doi:10.1002/(sici)1097-010x(19980301)280:4<277::aid-jez2>3.0.co;2-l

Cited by 16 publications

(13 citation statements)

References 42 publications

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“…The enterocyte mitochondria have previously been shown to be sensitive to malnutrition in cod and turbot larvae by swelling when larvae were fed diets containing soy phospholipids instead of marine phospholipids (MacQueen Leifson et al , 2003). Swollen mitochondria were observed in hepatocytes of turbot larvae (Segner & Witt, 1990), sea bream and pike‐perch Sander lucioperca (L.) (Diaz et al , 1998) and cod (G. Høvde, P.‐A. Wold & E. Kjørsvik, unpubl.…”

Section: Discussionmentioning

confidence: 99%

Allometric growth and functional development of the gut in developing cod Gadus morhua L. larvae

Wold

Hoehne‐Reitan

Rainuzzo

2008

Journal of Fish Biology

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The development of the gut epithelium in cod Gadus morhua was studied during the larval period in intensive rearing systems. Cod larvae were fed enriched rotifers from mouth opening. On 17 days post-hatch (dph) one group of larvae were fed Artemia sp. nauplii while another group were fed both rotifers and a formulated diet (co-fed). At the end of the experiment (30 dph) larvae receiving live feed were almost three times larger than the co-fed larvae, although no clear signs of pathological effects due to feeding regime were found in any larvae sampled for morphological studies. The midgut volume in larvae fed live feed increased by a factor of 38 during the experiment, and in particular volume increased rapidly between 24 and 30 dph. The enterocyte size increased between 12 and 24 dph from 652 AE 64 to 1479 AE 144 mm 3 (mean AE S.E.). When enterocytes reached their maximum size, several morphological changes in the gut epithelium were initiated, such as increased number of mitochondria per enterocyte, increased size of the nuclei and a considerable increase in microvilli surface area. The mitochondrial membrane structures changed during the experiment, suggesting a maturation process of the mitochondria. The midgut development was strongly related to larval size rather than age. On 30 dph co-fed larvae were equal in size to Artemia sp. fed larvae on 24 dph. This was reflected by equal values of estimated midgut volume, midgut length and total number of enterocytes and the number of mitochondria per enterocyte. The microvilli surface area, however, was significantly larger in co-fed larvae on 24 dph compared to live-feed larvae on 30 dph. This increase in absorptive surface was probably a response to suboptimal feeding conditions. The strong correlation between gut development and larval size and the lack of clear pathological effects, suggested that the gut tissue is flexible and can withstand periods of suboptimal nutrition at this stage.

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Section: Discussionmentioning

confidence: 99%

Allometric growth and functional development of the gut in developing cod Gadus morhua L. larvae

Wold

Hoehne‐Reitan

Rainuzzo

2008

Journal of Fish Biology

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“…However, none can actually sustain long life. Starved green-water larvae quickly present symptoms of nutritional deficiency (Diaz et al 1998) and die a few days after the clear-water controls. However, if these few extra days' survival represent additional time for initiation of successful exogenous feeding, these larvae would seem to have a considerable advantage over their clear-water counterparts.…”

Section: Drinking and Ingestion Of Dissolved Organicsmentioning

confidence: 99%

Uses of Microalgae in Aquaculture

Muller‐Feuga¹,

Robert²,

Cahu³

et al. 2003

Live Feeds in Marine Aquaculture

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“…The liver of fish larvae during the endogenous feeding phase, hepatocytes become spherical with central basophilic nuclei and slight eosinophilic homogeneous cytoplasm (Gisbert, Moreira, Castro‐Ruiz, Öztürk, Fernández, Gilles, Nuñez, Duponchelle, Tello, Renno, García‐Dávila & Darias ). After the onset of exogenous feeding, hepatocytes increase in size and number, achieving a shape polyhedral, and more eosinophilic, and disposed tightly packed between sinusoids, often around a central vein (Deplano, Diaz, Connes, Kentouri‐Divanach & Cavalier ; Diaz, Mani‐Ponset, Guyot & Connes ; Gisbert et al . ).…”

Section: Introductionmentioning

confidence: 99%

Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans ) reared on intensive fish farming

2017

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This study aimed to characterize the liver histology and histomorphometry in sorubim hybrid of different categories (nursery, growth and grow-out) reared on fish farming. The categories were defined considering body weight (BW): nursery category (n = 5): BW = 37.06 AE 6.00 g (31.6-45.3 g); growth category (n = 5): BW = 310.40 AE 53.80 g (242.1-376.4 g) and grow-out category (n = 5): BW = 874.28 AE 27.59 g (846.2-913.1 g). Liver fragments were processed to paraffin inclusion, and sections were stained by haematoxylin and eosin (H&E), PAS (Periodic Acid Schiff) and Perl's staining to histology, histomorphometry and density volumetric of liver structures; glycogen analysis and to detect ferric irons (Fe 3+ ) respectively. The hepatosomatic index decreased between the categories (P < 0.01). The percentage of PAS-positive hepatocytes in the nursery category was higher (P < 0.05) in relation to the growth and grow-out categories. The hepatocytes from all fish were positive to Perl's staining. The density volumetric of liver structures did not differ among categories except to blood vessels were higher (P < 0.01) in the nursery and growth. The area (lm 2 ) and perimeter (lm) of hepatocytes, and the area (lm 2 ), perimeter (lm) and volume (lm 3 ) of the nuclei from grow-out fish were lower (P < 0.01) than those from the nursery and growth categories. Changes in morphometric characteristics of hepatocytes may result from metabolic changes associated with body growth surubins; therefore, these morphometric characteristics of liver tissue can be used as functional biomarkers for the assessment of fish health and nutrition status.

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Hepatic cholestasis during the post-embryonic development of fish larvae

Cited by 16 publications

References 42 publications

Allometric growth and functional development of the gut in developing cod Gadus morhua L. larvae

Allometric growth and functional development of the gut in developing cod Gadus morhua L. larvae

Uses of Microalgae in Aquaculture

Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans ) reared on intensive fish farming

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