Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum  × Pseudoplatystoma corruscans ) reared on intensive fish farming

Abstract

This study aimed to characterize the liver histology and histomorphometry in sorubim hybrid of different categories (nursery, growth and grow-out) reared on fish farming. The categories were defined considering body weight (BW): nursery category (n = 5): BW = 37.06 AE 6.00 g (31.6-45.3 g); growth category (n = 5): BW = 310.40 AE 53.80 g (242.1-376.4 g) and grow-out category (n = 5): BW = 874.28 AE 27.59 g (846.2-913.1 g). Liver fragments were processed to paraffin inclusion, and sections were stained by haematoxylin and eosin (H&E), PAS (Periodic Acid Schiff) and Perl's staining to histology, histomorphometry and density volumetric of liver structures; glycogen analysis and to detect ferric irons (Fe 3+ ) respectively. The hepatosomatic index decreased between the categories (P < 0.01). The percentage of PAS-positive hepatocytes in the nursery category was higher (P < 0.05) in relation to the growth and grow-out categories. The hepatocytes from all fish were positive to Perl's staining. The density volumetric of liver structures did not differ among categories except to blood vessels were higher (P < 0.01) in the nursery and growth. The area (lm 2 ) and perimeter (lm) of hepatocytes, and the area (lm 2 ), perimeter (lm) and volume (lm 3 ) of the nuclei from grow-out fish were lower (P < 0.01) than those from the nursery and growth categories. Changes in morphometric characteristics of hepatocytes may result from metabolic changes associated with body growth surubins; therefore, these morphometric characteristics of liver tissue can be used as functional biomarkers for the assessment of fish health and nutrition status.

Reproductive physiology of Rhamdia quelen is improved by dietary inclusion of probiotics

“…The liver's histomorphometry was performed by determining the number of hepatocytes per area (µm 2 ), diameter of hepatocyte nucleus (µm) and Rpcn (perimeter of the nucleus/ cytoplasm), according to Rodrigues, Saturnino, et al. (2017). Morphological alterations were evaluated qualitatively, by means of the lesion index of Bernet et al.…”

Effects of Bac-Tratâ® Probiotic Complex on Growth, Hematological and Intestinal Parameters of Nile Tilapia, Reared at Low Temperatures

“…Intestinal villi histomorphometry was performed by measuring ten villi's height (from base to top) and width of the folds (close to apex region) per sample, and the thickness of the muscle tunic (smooth muscle) in ten points per sample, using 40x and 100x lens. Histomorphometry was performed according to Rodrigues et al (2017), from five randomly selected pictures with a 1000x magnification. The area (µm 2 ), perimeter and diameter (µm) of the hepatocytes nuclei were measured in 50 cells per fish, aiming to calculate the volume of the nucleus Vnh (µm 3 ) = 4/3 π.r 3 , where r is the nuclear ray.…”

“…The analyses of histomorphometric measurements of the area of hepatocyte nuclei are used as biomarkers, aiming to observe the state and metabolic activity of cells (Rodrigues et al, 2017).…”

“…The low histomorphometric values of hepatocyte nuclei found in treatment T2 may be related to a nutritional stress status, as suggested by Wold et al (2009), who reported that fish submitted to nutritional stresses suffers reduction of the size of hepatocytes. Rodrigues et al (2017), when studying the liver of a surubim hybrid (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans), observed that inadequate feeding reduces hepatocyte's metabolism, and consequently the nuclear area.…”

Comparative liver morphology associated with the hepatosomatic index in five Neotropical anuran species

“…Conversely, histometric techniques allow us to quantify organ structure density for a given set of histological sections (Marcos, Monteiro, & Rocha, 2012; Weibel, Kistler, & Scherle, 1966). For example, in fish, changes in hepatocyte volume are associated with metabolic status during body development, while parenchymal density may suffer seasonal and sex effects as a natural adaptative process (Rocha, Monteiro, & Pereira, 1997; Rodrigues, Saturnino, & Fernandes, 2017). In anurans, however, histometric traits have not been explored comparatively among different species.…”