Heat shock protein 70 response to physical and chemical stress in Chamelea gallina

Monari, Marta; Foschi, Jurgen; Rosmini, Roberto; Marin, María Gabriella; Serrazanetti, Gian Paolo

doi:10.1016/j.jembe.2010.11.016

Cited by 42 publications

(20 citation statements)

References 49 publications

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“…Previous studies have demonstrated that overexpression of Hsp70 by gene transfection in the animal could preserve the activity and content of Mn-SOD (Tupling et al 2008). And the overexpression of Hsp70 was observed in physical and chemical stress of Chamelea gallina (Monari et al 2011). Consistent with these prior studies, mRNA and protein levels of Hsp70 in immune organs were upregulated in the present study.…”

Section: Discussionmentioning

confidence: 96%

Cold stress induces antioxidants and Hsps in chicken immune organs

Zhao

Zhang

et al. 2014

Cell Stress and Chaperones

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The aim of this study was to investigate the effects of cold stress on oxidative indexes, immune function, and the expression levels of heat shock protein (Hsp90, Hsp70, Hsp60, Hsp40, and Hsp27) in immune organs of chickens. Two hundred forty 15-day-old male chickens were randomly divided into 12 groups and kept under the temperature of (12± 1)°C for acute and chronic cold stress. There were one control group and five treatment groups for acute cold stress and three control groups and three treatment groups for chronic cold stress. The results showed that cold stress influence the activities of antioxidant enzymes in the immune organs. The activities of SOD and GSH-Px were first increased then decreased, and activity of total antioxidation capacity (T-AOC) was significantly decreased (P<0.05) at the acute cold stress in chicks; however, T-AOC activities were significantly increased (P<0.05) at the chronic cold stress in these tissues. Cold stress induced higher level of malondialdehyde (MDA) in chicken immune organs. In addition, the cytokine contents were increased in cold stress groups. As one protective factor, the expression levels of Hsps were increased significantly (P<0.05) in both cold stress groups. These results suggested that cold stress induced the oxidative stress in the three tissues and influenced immune function of chicks. Higher expression of Hsps (Hsp90, Hsp70, Hsp60, Hsp40, and Hsp27) may play a role in protecting immune organs against cold stress.

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Section: Discussionmentioning

confidence: 96%

Cold stress induces antioxidants and Hsps in chicken immune organs

Zhao

Zhang

et al. 2014

Cell Stress and Chaperones

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“…The family HSP70 is one of the most studied and several studies have shown its induction in stress conditions by heavy metals (Köhler et al, , 1996bNadeau et al, 2001;Zanger et al, 1996). Monari et al (2011) worked with the mollusc species Chamelea gallina, observing an increase in the expression of HSP70. The authors affirm that the induction of HSP70 can be considered an adaptation mechanism associated with changes in the environmental parameters.…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Bioindicators and Biomarkers in the Assessment of Soil Toxicity

Fontanetti¹,

Nogarol²,

Souza³

et al. 2011

Soil Contamination

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“…Impaired stress protein function associated with reduced ATP levels has been previously established (Feige et al, 1996;Roberts et al, 1997). It has been postulated that energy depletion caused by increased osmoregulation under low salinity conditions might lead to reduced levels of HSP70 proteins in the swimming crab, and has also been observed in all tissues of P. amurensis (Werner and Hinton, 2000) and in the gill of Chamelea gallina (Monari et al, 2011). In the current study, as time progressed, HSP70 expression increased to protect organisms from further damage (de la Vega et al, 2006).…”

Section: Discussionmentioning

confidence: 84%

“…The involvement of HSP genes in both high and low salinity responses has been demonstrated in many aquatic animals. It has been demonstrated that the expression level of HSP70 gradually increases after low salinity exposure in the gill of Mytilus galloprovincialis (Hamer et al, 2004), but decreases in the gill of Chamelea gallina (Monari et al, 2011). The overexpression of HSP70 has also been re-X.N.…”

Section: Discussionmentioning

confidence: 99%

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Bao

Zhang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72 h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70, HSP90-1, HSP90-2) in the hepatopancreas of wild (WG) and screened (SG) groups of P. trituberculatus exposed to low salinity (4 ppt). The results showed that 3 of the candidate genes (HSP60, HSP70, HSP90-1) exhibited similarly downregulated expression profiles in the first 3 h (P < 0.05), which became upregulated from 3 h to 72 h after being subjected to low salinity conditions. In contrast, the expression profile of the HSP90-2 gene was upregulated during the first 6 h for the WG, and during the first 12 h for the SG, after which it became downregulated. HSP90-1 and HSP90-2 were highly expressed at 12 h after low salinity challenge in the SG, but not the WG. The response of these 2 genes to salinity stress indicates their suitability as biomarkers to differentiate SG from WG crabs. The results indicate that HSP genes are involved in the adaptation of crabs to low salinity exposure, and that different HSPs have diverse functions in response to low salinity stress in P. trituberculatus. In addition, HSP expression in SG indicates that this group is more tolerant to low salinity conditions compared to WG.

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Heat shock protein 70 response to physical and chemical stress in Chamelea gallina

Cited by 42 publications

References 49 publications

Cold stress induces antioxidants and Hsps in chicken immune organs

Cold stress induces antioxidants and Hsps in chicken immune organs

Bioindicators and Biomarkers in the Assessment of Soil Toxicity

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

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