mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Bao, Xingjie; Mu, Changkao; Zhang, C.; Wang, Y.F.; Song, Weiwei; Li, R.H.

doi:10.4238/2014.august.29.5

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…In M. japonicus gills, low salinity down-regulated the mRNA expression of most HSPs, but not HSP60. The expression levels of HSP70, HSP60, and HSP90 also exhibit down-regulation in the swimming crab P. trituberculatus following low salinity stress [32]. In Yang,experiment, the expression of HSP60 and HSP70 was signi cantly upregulated, while other HSPs decreased or had no signi cant change at low temperatures in cray sh.…”

Section: Discussionmentioning

confidence: 92%

Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon

Yang

Jiang

et al. 2023

Preprint

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In order to explore the influence of temperature and salinity on stress response of P. monodon, four experimental groups were set, respectively were normal temperature and normal salt (Control group), normal temperature and low salt(Group A), high temperature and low salt(Group B), high temperature and normal salt(Group C). The stress experiment was conducted for 96 hours, and the activities of acid phosphatase(ACP), alkaline phosphatase(AKP), total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) in hepatopancreas, the changes of tissue structure and the expression of related heat shock protein genes were measured．The results showed that the branchial structures of the three stress groups were found to be damaged to varying degrees. In Group A, AKP and ACP levels increased initially and then decreased, while T-SOD levels decreased initially and then steadily increased. In Group B and Group C, the four enzymes showed similar trends.There was no significant difference in the expression level of Hsps in the low salt group. The expression levels of Hsp-10, Hsp-60 and Hsp-90 genes showed a similar trend, and the maximum expression levels of the four genes were all at 96h. The expression levels of Hsps in muscle tissue were similar to those in hepatopancreas tissue. However, the highest expression levels of Hsp-70 and Hsp-90 in high temperature and low salt group were at 24h. Through this work on P. monodon stress responses to high temperature and low salinity, we have gained a deeper understanding of the physiological responses to environmental stressors, which will be useful in the development of protective measures for the shrimps under aquaculture conditions that replicate these stressors.

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Section: Discussionmentioning

confidence: 92%

Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon

Yang

Jiang

et al. 2023

Preprint

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“…Generally, upregulation of HSP70 expression is described 2 or 3 h after the beginning of the stress protocol (Bao et al, 2014;Fu et al, 2013). To our knowledge, this is the first study to evaluate HSP70 gene expression after chasing stress in a crustacean.…”

Section: Discussionmentioning

confidence: 93%

“…The heat shock response (HSR), characterized by the increase in the expression and activity of heat shock proteins (HSPs) or stress proteins, is another pathway triggered not only by temperature alterations but also by different types of environmental stresses, such as alterations in salinity and exposure to xenobiotics (Bao et al, 2014; Baringou et al, 2016; Chaurasia et al, 2016; Cottin et al, 2015; Fernandes et al, 2018; Ortega & Vitorino, 2022). HSPs comprise a family of highly conserved proteins ubiquitously present in all living organisms, from bacteria to mammals (Bao et al, 2014; Baringou et al, 2016; Chaurasia et al, 2016). Therefore, HSR is considered an ecological and physiological biomarker of stress (Bao et al, 2014; Liu et al, 2018; Moreira‐de‐Sousa et al, 2018; Qian et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…HSPs comprise a family of highly conserved proteins ubiquitously present in all living organisms, from bacteria to mammals (Bao et al, 2014; Baringou et al, 2016; Chaurasia et al, 2016). Therefore, HSR is considered an ecological and physiological biomarker of stress (Bao et al, 2014; Liu et al, 2018; Moreira‐de‐Sousa et al, 2018; Qian et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

et al. 2023

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Ocypode quadrata, a Ghost crab species found along the western Atlantic coast, is considered a bioindicator of anthropogenic impact on sandy beaches. Ghost Crabbing, a touristic activity in which ghost crabs are chased just for fun, is a potentially threatening activity for this crab. In crustaceans, metabolites such as glucose and lactate, and the gene expression of crustacean hyperglycemic hormone (CHH) and heat shock proteins (HSPs) increase when the animals are exposed to several types of stress, including alterations in temperature, salinity, or exposure to xenobiotics. This work was developed to identify if being chased by humans would affect these markers of stress in this species of crab. The effects of chasing stress on hemolymph and tissue metabolites and the gene expression levels of CHH and HSP70 were investigated. The levels of lactate in the hemolymph of stressed crabs were six times higher than those of control crabs immediately after chasing and decreased progressively during recovery, indicating an active anaerobic metabolism during the stress. On the contrary, glucose levels in the hemolymph of the stressed crabs increased progressively from 30 to 60 min after chasing, indicating an inverse correlation between glucose and lactate and the conversion of lactate to glucose by gluconeogenesis. In stressed crabs, the levels of triglycerides in the hemolymph decreased 30 min after chasing, while the opposite tended to occur in the hepatopancreas, indicating that during recovery, the crabs use triglycerides as energy source to sustain aerobic metabolism. Finally, this study demonstrates that ghost crabs are stressed by minimum human contact and that “ghost crabbing” must not be encouraged as a tourist activity.

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“…In a third study in which C. sapidus was fed TBT-contaminated food, the respiration rate, the expression of P-450 3A (CYP3A), and heat shock proteins (HSPs) in the hepatopancreas increased, indicating that the crabs were stressed by TBT ( 51 ). An active heat shock response, specially with increased HSP70 expression, occurs when crustaceans are exposed to many types of environmental stress such as heat ( 55 – 58 ), metals ( 59 , 60 ), and salinity alterations ( 61 , 62 ). Therefore, increased expression of HSPs could be a useful indicator of BTs/TBT contamination that should be studied in other crustacean species (Figure 2 ).…”

Section: Ots Effects On the Metabolismmentioning

confidence: 99%

Effects of Organotins on Crustaceans: Update and Perspectives

2018

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Organotins (OTs) are considered some of the most toxic chemicals introduced into aquatic environments by anthropogenic activities. They are widely used for agricultural and industrial purposes and as antifouling additives on boat hull’s paints. Even though the use of OTs was banned in 2008, elevated levels of OTs can still be detected in aquatic environments. OTs’ deleterious effects upon wildlife and experimental animals are well documented and include endocrine disruption, immunotoxicity, neurotoxicity, genotoxicity, and metabolic dysfunction. Crustaceans are key members of zooplankton and benthic communities and have vital roles in food chains, so the endocrine-disrupting effects of tributyltin (TBT) on crustaceans can affect other organisms. TBT can disrupt carbohydrate and lipid homeostasis of crustaceans by interacting with retinoid X receptor (RXR) and crustacean hyperglycemic hormone (CHH) signaling. Moreover, it can also interact with other nuclear receptors, disrupting methyl farnesoate and ecdysteroid signaling, thereby altering growth and sexual maturity, respectively. This compound also interferes in cytochrome P450 system disrupting steroid synthesis and reproduction. Crustaceans are also important fisheries worldwide, and its consumption can pose risks to human health. However, some questions remain unanswered. This mini review aims to update information about the effects of OTs on the metabolism, growth, and reproduction of crustaceans; to compare with known effects in mammals; and to point aspects that still needs to be addressed in future studies. Since both macrocrustaceans and microcrustaceans are good models to study the effects of sublethal TBT contamination, novel studies should be developed using multibiomarkers and omics technology.

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mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Cited by 10 publications

References 33 publications

Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon

Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

Effects of Organotins on Crustaceans: Update and Perspectives

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