Healing of a soft tissue wound of the neck and jaw osteoradionecrosis using platelet gel

Piccin, Andrea; Pierro, Angela Maria Di; Tagnin, Mario; Russo, Carla; Fustos, R.; Corvetta, Daisy; Primerano, Marco; Magri, Elena; Conci, Viviana; Gentilini, I; Stöcker, E.; Negri, Giovanni; Mazzoleni, Guido; Gastl, Günther; Fontanella, Fabrizio

doi:10.2217/rme-2016-0031

Cited by 28 publications

(19 citation statements)

References 12 publications

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“…Future studies should also focus to identify what kind of clinically available growth factors (e.g., platelet gel [24][25][26][27][28]) may be used to improve the healing processes.…”

Section: Discussionmentioning

confidence: 99%

“…Their common action is PG enriched with local growth factors (vascular-endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor beta 1 (TGF-β1) and other) potentiating stem cells [24]. This became popular for the treatment of skin ulcers, radiodermatitis and other conditions [25][26][27]. Recently, a case of life-threatening oral mucositis (OM) following high-dose conditioning chemotherapy for peripheral blood stem cell transplantation (PBSCT), which was successfully treated with cord blood platelet gel (CBPG) was described [28].…”

Section: Introductionmentioning

confidence: 99%

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Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry

et al. 2020

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Background and objectives: Subepithelial connective tissue graft (SCTG) from the palate has been considered as the “gold standard” for the treatment of deep gingival recessions. A single-incision technique was reported to allow primary wound healing. A palatal single incision was performed in a rat model. The present study assessed the histology and histomorphometry of palatal wound healing following surgical closure with primary intention. Materials and Methods: Twenty-six 6-month-old male Wistar rats weighing 427–650 g. An incision was made on the maxillary palate. A full thickness flap was raised palatally, and then repositioned and sutured. Two experimental groups: S—Study group, I—Intact control group. Half of the animals were sacrificed 7 days and the remaining 14 days postoperatively. Outcome parameters included—epithelial gap; inflammatory infiltration; vascular fraction, expression of myofibroblasts and stem cell markers within the oral epithelium and stromal cells and physical properties of stromal collagen fibers. Investigations were performed at two time-points (7 and 14 days) during the wound healing process. Results: The epithelial gap closed completely after 14 days. The inflammatory reaction and vascular fraction were relatively low. Surgical trauma downregulated the expression of cytokeratin (CK) 14 and CK 15, which returned to normal after 14 days. Epithelial differentiation was mediated through upregulation of connective tissue sex- determining-region-Y-box2 (SOX2). Epithelial SOX2, CD34, alpha smooth muscle actin (αSMA) and physical properties of stromal collagen fibers were not influenced by the surgical trauma. Conclusions: Surgical trauma followed by palatal wound healing with primary intention in a rat model heals within 14 days. It induces minimal inflammatory infiltration and vascular proliferation. Epithelization is exerted through promotion of epithelial differentiation from stem cells by connective tissue SOX2.

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“…Future studies should also focus to identify what kind of clinically available growth factors (e.g., platelet gel [24][25][26][27][28]) may be used to improve the healing processes.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry

et al. 2020

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“…Currently, the optimal way to manage osteoradionecrosis is unknown . Several case reports and large patient series documenting the use of PRP for the effective treatment of osteoradionecrosis have been published . A limited number of prospective studies using PRP have been performed with mixed results.…”

Section: Discussionmentioning

confidence: 99%

Plasma‐based biomaterials for the treatment of cutaneous radiation injury

Miller

Song

Smith

et al. 2018

Wound Repair Regeneration

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Cutaneous wounds caused by an exposure to high doses of ionizing radiation remain a therapeutic challenge. While new experimental strategies for treatment are being developed, there are currently no off-the-shelf therapies for the treatment of cutaneous radiation injury that have been proven to promote repair of the damaged tissues. Plasmabased biomaterials are biologically active biomaterials made from platelet enriched plasma, which can be made into both solid and semi-solid forms, are inexpensive, and are available as off-the-shelf, nonrefrigerated products. In this study, the use of plasmabased biomaterials for the mitigation of acute and late toxicity for cutaneous radiation injury was investigated using a mouse model. A 2-cm diameter circle of the dorsal skin was irradiated with a single dose of 35 Gy followed by topical treatment with plasmabased biomaterial or vehicle once daily for 5 weeks postirradiation. Weekly imaging demonstrated more complete wound resolution in the plasma-based biomaterial vs. vehicle group which became statistically significant (p < 0.05) at weeks 12, 13, and 14 postmaximum wound area. Despite more complete wound healing, at 9 and 17 weeks postirradiation, there was no statistically significant difference in collagen deposition or skin thickness between the plasma-based biomaterial and vehicle groups based on Masson trichrome staining nor was there a statistically significant difference in inflammatory or fibrosis-related gene expression between the groups. Although significant improvement was not observed for late toxicity, plasma-based biomaterials were effective at promoting wound closure, thus helping to mitigate acute toxicity.

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“…In addition, Piccin A used platelet gel to treat soft tissue wounds in the neck and jaws of osteoporosis in the same year . In 2017, Piccin et al highlighted the potential of platelet gel as a promising therapeutic tool for clinical use . After hematopoietic stem cell transplantation, the tissue regeneration of severe oral mucositis was reused .…”

Section: Discussionmentioning

confidence: 99%

Autologous blood transfusion stimulates wound healing in diabetic mice through activation of the HIF‐1α pathway by improving the blood preservation solution

Zhu

Yongquan

et al. 2020

FASEB j.

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Transfusion of autologous blood is a timesaving, convenient, safe, and effective therapy from a clinical perspective, and often employed for the treatment of diabetic patients. Stabilization of HIF‐1α has been widely reported to be a critical factor in the improvement of wound healing in diabetes. Therefore, our study reveals the roles of improved autologous blood in wound healing in diabetes, through autologous blood transfusion in a mouse model. Initially, BALB/c mice were subjected to streptozotocin for diabetic mouse model establishment. Diabetic mice were transfused with improved or standard autologous blood in perfusion culture system. Roles of improved autologous blood in mediating HIF‐1α pathway were determined by measuring expression of VEGF, EGF, HIF‐1α, and HSP‐90. In order to assess the detailed regulatory mechanism of improved autologous blood in perspective of wound healing, cell proliferation, migration and cell cycle, fibroblasts isolated from diabetic mice were transfected with HIF‐1α siRNA. Mice transfused with improved autologous blood exhibited increased levels of CD31 and α‐SMA in skin tissues, and reduced TNF‐α, IL‐1β, and IL‐6 levels, indicating that improved autologous blood promoted wound healing ability and reduced the release of inflammatory factors. Diabetic mice transfused with improved autologous blood presented activated HIF‐1α pathway. The survival rate, proliferation, and migration of fibroblasts were elevated via activation of the HIF‐1α pathway. Taken together, improved blood preservation solution could enhance the oxygen carrying capacity of red blood cells and wound healing in mice with diabetes, which is achieved through regulation of HIF‐1α pathway.

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Healing of a soft tissue wound of the neck and jaw osteoradionecrosis using platelet gel

Abstract: Additional studies are needed to confirm the promising effect of PLT-gel for the treatment of osteoradionecrosis.

Cited by 28 publications

References 12 publications

Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry

Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry

Plasma‐based biomaterials for the treatment of cutaneous radiation injury

Autologous blood transfusion stimulates wound healing in diabetic mice through activation of the HIF‐1α pathway by improving the blood preservation solution

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