Plasma‐based biomaterials for the treatment of cutaneous radiation injury

Abstract

Cutaneous wounds caused by an exposure to high doses of ionizing radiation remain a therapeutic challenge. While new experimental strategies for treatment are being developed, there are currently no off-the-shelf therapies for the treatment of cutaneous radiation injury that have been proven to promote repair of the damaged tissues. Plasmabased biomaterials are biologically active biomaterials made from platelet enriched plasma, which can be made into both solid and semi-solid forms, are inexpensive, and are available as off-the-shelf, nonrefrigerated products. In this study, the use of plasmabased biomaterials for the mitigation of acute and late toxicity for cutaneous radiation injury was investigated using a mouse model. A 2-cm diameter circle of the dorsal skin was irradiated with a single dose of 35 Gy followed by topical treatment with plasmabased biomaterial or vehicle once daily for 5 weeks postirradiation. Weekly imaging demonstrated more complete wound resolution in the plasma-based biomaterial vs. vehicle group which became statistically significant (p < 0.05) at weeks 12, 13, and 14 postmaximum wound area. Despite more complete wound healing, at 9 and 17 weeks postirradiation, there was no statistically significant difference in collagen deposition or skin thickness between the plasma-based biomaterial and vehicle groups based on Masson trichrome staining nor was there a statistically significant difference in inflammatory or fibrosis-related gene expression between the groups. Although significant improvement was not observed for late toxicity, plasma-based biomaterials were effective at promoting wound closure, thus helping to mitigate acute toxicity.

Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models

“…According to Veith et al, reactive free radicals activate the vascular endothelial growth factor during angiogenesis. Furthermore, reactive spices are used for wound sterilization (20)(21)(22). In a study by Isbary et al exploring argon plasma in chronic wound healing, no abnormal side effects were observed in tissues (23) and, similar to our study, visceral tissues were not damaged by helium plasma radiation during histopathological examination.…”

Does survivin overexpression enhance the efficiency of fibroblast cell-based wound therapy?

“…Both VEGF and bFGF are incredibly involved in the formation of new blood vessels [10]. One of the common scaffolds for efficient cell delivery in tissue engineering is plasma-based hydrogels [11]. Fibrin clot (also known as fibrin gel) is such a scaffold that self-assembles into a polymer network acting as a suitable cell carrier.…”

Preclinical modeling of low energy X-rays radiological burn: Dosimetry study by monte carlo simulations and EPR spectroscopy

“…Many preclinical models have already been proposed in the literature in both rats and mice to improve knowledge and to better understand the radiopathological specificities of ionizing radiation on skin, muscle and/or bone tissues using different qualities of radiation ( Lerouxel et al, 2009 ; Jang et al, 2016 ; Rottensteiner-Brandl et al, 2017 ; Miller et al, 2019 ; Zhai et al, 2019 ; Dos Santos et al, 2021b ). With the democratization of the use of conventional X-rays cabinets or dedicated image-guided platforms, these types of facilities are more and more widely used for radiobiological studies ( Brown et al, 2022 ) and are particularly well-suited for our study as they involve low-energy X-rays.…”