Background and objectives: Subepithelial connective tissue graft (SCTG) from the palate has been considered as the “gold standard” for the treatment of deep gingival recessions. A single-incision technique was reported to allow primary wound healing. A palatal single incision was performed in a rat model. The present study assessed the histology and histomorphometry of palatal wound healing following surgical closure with primary intention. Materials and Methods: Twenty-six 6-month-old male Wistar rats weighing 427–650 g. An incision was made on the maxillary palate. A full thickness flap was raised palatally, and then repositioned and sutured. Two experimental groups: S—Study group, I—Intact control group. Half of the animals were sacrificed 7 days and the remaining 14 days postoperatively. Outcome parameters included—epithelial gap; inflammatory infiltration; vascular fraction, expression of myofibroblasts and stem cell markers within the oral epithelium and stromal cells and physical properties of stromal collagen fibers. Investigations were performed at two time-points (7 and 14 days) during the wound healing process. Results: The epithelial gap closed completely after 14 days. The inflammatory reaction and vascular fraction were relatively low. Surgical trauma downregulated the expression of cytokeratin (CK) 14 and CK 15, which returned to normal after 14 days. Epithelial differentiation was mediated through upregulation of connective tissue sex- determining-region-Y-box2 (SOX2). Epithelial SOX2, CD34, alpha smooth muscle actin (αSMA) and physical properties of stromal collagen fibers were not influenced by the surgical trauma. Conclusions: Surgical trauma followed by palatal wound healing with primary intention in a rat model heals within 14 days. It induces minimal inflammatory infiltration and vascular proliferation. Epithelization is exerted through promotion of epithelial differentiation from stem cells by connective tissue SOX2.
Background: Recent evidence suggests that bone marrow-derived stem cells may have an important role in the natural process of wound healing. The aim of the present study was to examine the effect of orabase paste application on primary wound healing in a palatal rat model. Materials and Methods:A total of 48 six-month-old male Wistar rats weighting 427 to 650 g were included. A mid-crestal incision was made on the maxillary alveolar ridge. A full-thickness flap was raised on either side of the incision and was then repositioned and sutured. Three experimental groups were used: Ostudy group-orabase, N-negative control group, and I-intact control group. Half of the animals were killed on 7 days and the remaining on 14 days postoperatively. Outcome parameters included epithelial gap; inflammatory infiltration; expression of stem cell markers within the oral epithelium and stromal cells; and physical properties of stromal collagen fibers and myofibroblasts. Investigations were performed at 2 time points (7 and 14 d) during the wound healing process. Results:The epithelial gap closed completely after 7 days in the O group versus 14 days or more in the N group. The inflammatory reaction was relatively low and not significantly different between groups O and N. Orabase upregulated the expression of CK14, CK15, and epithelial SOX2. Connective tissue SOX2, CD34, and α-smooth muscle actin and physical properties of stromal collagen fibers were not influenced by the application of orabase.Conclusions: Orabase promotes epithelial gap closure in a primary wound healing model in rats. The effect is exerted through promotion of epithelial differentiation from stem cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.