Graphene Oxide Immobilized PLGA‐polydopamine Nanofibrous Scaffolds for Growth Inhibition of Colon Cancer Cells

Abstract

Graphene oxide (GO)/poly (lactide‐co‐glycolic acid) (PLGA) scaffolds have promising applications in the biomedical field. However, greater attention is focused on the incorporated system and its applications in normal cells. In this work, a novel GO immobilized PLGA nanofibrous scaffold assisted by polydopamine (PLGA‐PDA‐GO) is developed for growth inhibition of HT‐29 colon cancer cells. The interactions between GO and PDA are attributed to a π–π conjugate interaction and electrostatic attraction. In addition to the enhancement of thermal stability and mechanical strength, the surface roughness, hydrophilicity, and electro‐activity of the scaffolds are significantly improved by immobilization of GO. The scaffolds show good inhibition of HT‐29, and immobilized GO is observed to be in contact with but not internalized in HT‐29 cells. The cytotoxicity mechanism of scaffolds toward HT‐29 is attributed to intracellular activated reactive oxygen species that result from the physical interaction of the sharp GO edges and electrical signals of π–π stacking between PDA and GO.

Enwrapping Polydopamine on Doxorubicin-Loaded Lamellar Hydroxyapatite/Poly(lactic-co-glycolic acid) Composite Fibers for Inhibiting Bone Tumor Recurrence and Enhancing Bone Regeneration

“…The peaks located at 1181 and 1085 cm −1 are due to the stretching vibration of the C−O−C bond in PLGA. 29,30 In the spectra of DH/PLGA and PDA@DH/ PLGA, the peaks at 560 and 590 cm −1 correspond to the bending vibration of the O−P−O bond in LHAp. 15 Apart from the characteristic peaks of PLGA and LHAp, PDA@DH/ PLGA exhibits additional peaks at 1625 and 1509 cm −1 , which are due to the overlap of CC resonance vibrations in the aromatic ring and N−H bending vibrations and the stretching vibration of the N−H bond in PDA 18,31 (Figure 3b), indicating successful deposition of PDA on the fiber surface.…”

Promising Graphene-Based Nanomaterials and Their Biomedical Applications and Potential Risks: A Comprehensive Review

“…Linear polymerides incorporate poly- d , l -lactic- co -glycolic acid (PLGA), PEG, polydopamine (PDA), PVP, poly­(acrylic acid) (PAA), polymethacrylic acid (PMAA), polylactic acid (PLA), and polypyrrole (PPy). Among them, PLGA and PEG have been successfully used in drug and gene delivery at the same time. − Notably, PEG has become the most commonly used linear polymer for GBN modification since Liu et al prepared a delivery material capable of adsorbing the hydrophobic aromatic molecule camptothecin analogue SN38 using PEG and GO in 2008. , This is possible because GBNs modified by biocompatible neutral charged PEG have high hydrophilia and physiological stability in serum and other physiological conditions.…”

CGRP‐Loaded Porous Microspheres Protect BMSCs for Alveolar Bone Regeneration in the Periodontitis Microenvironment

“…Characteristic peaks of PLGA spectrum were located at 1183, 1088, and 1269 cm −1 (symmetric C─O─C stretching in the ester group); 1381 cm −1 (─CH 3 stretching); 1452, 2990, and 2940 cm −1 (─CH 2 stretching); and 1750 cm −1 (C═O stretching vibration). [ 42 ] Compared with PLGA, PLGA/PDA spectrum was characterized by peaks at 1621 cm −1 (aromatic ring skeleton stretching), and a broad absorbance at 3600–3100 cm −1 (the coupling of O─H and N─H stretching from PDA). [ 42 ] XRD was used to characterize the crystalline structure of the obtained PMs (Figure 4F).…”