Enwrapping Polydopamine on Doxorubicin-Loaded Lamellar Hydroxyapatite/Poly(lactic-co-glycolic acid) Composite Fibers for Inhibiting Bone Tumor Recurrence and Enhancing Bone Regeneration

Abstract

Simultaneous prevention of bone tumor recurrence and promotion of repairing bone defects resulting from tumorectomy remain a challenge. Herein, we report a polydopamine (PDA)-coated composite scaffold consisting of doxorubicin (DOX)-loaded lamellar hydroxyapatite (LHAp) and poly(lacticco-glycolic acid) (PLGA) in an attempt to reach dual functions of tumor inhibition and bone repair. The DOX was intercalated into LHAp, and the DOX-loaded LHAp was incorporated into PLGA solution to prepare a DOX-intercalated LHAp/PLGA (labeled as DH/PLGA) scaffold that was coated with PDA to obtain a PDA@ DH/PLGA scaffold. The morphology, structure, wettability, mechanical properties, drug release, biocompatibility, and in vitro and in vivo bioactivities of the PDA@DH/PLGA scaffold were evaluated. It is found that PDA coating not only improves hydrophilicity and mechanical properties, but also leads to more sustainable drug release. More importantly, the PDA@DH/PLGA scaffold shows significantly inhibited growth of tumor cells initially and subsequent improved adhesion and proliferation of osteoblasts. In addition, the PDA coating improves the bioactivity of the DH/PLGA scaffold as suggested by the in vitro biomineralization. Further in vivo study demonstrates the improved bone growth around PDA@DH/PLGA over DH/PLGA after 20 days of drug release. The dual functional PDA@DH/PLGA scaffold shows great promise in the treatment of bone tumor.

Smart-Temporary-Film-Based Local-Delivery System with Controllable Drug-Release Behavior

“…As seen in Figure 5 C,D, they showed quite similar release profiles as those at pH = 5.6. However, they all showed slower DOX release rates and lower release amounts than those at pH = 7.4, which agrees with the reported results [ 29 , 30 ]. The reason could be that DOX is protonated under acidic conditions.…”

Recent Advances in Hydroxyapatite-Based Biocomposites for Bone Tissue Regeneration in Orthopedics

“…The DOX @ PLGA-PDA-HA scaffold significantly inhibited tumor cell growth and enhanced osteoblast adhesion and proliferation. Furthermore, the PDA coating improved the bioactivity of the scaffold, as suggested by biomineralization in vitro [ 97 ].…”

Nano-Hydroxyapatite Composite Scaffolds Loaded with Bioactive Factors and Drugs for Bone Tissue Engineering

“…PDA coating not only improves the hydrophilicity and mechanical properties of the material but also prolongs the drug release time and inhibits the growth of tumor cells and promotes the proliferation of MC3T3-E1 cells. In addition, in vivo experiments in mice show that coating PDA on DOX@LHAp/PLGA scaffold can greatly promote bone growth [ 157 ].…”