Glyphosate and aminomethylphosphonic acid (AMPA) residues in Brazilian honey

Souza, Ana Paula Ferreira de; Rodrigues, Nádia Regina; Reyes, Felix G. R.

doi:10.1080/19393210.2020.1855676

Cited by 28 publications

(11 citation statements)

References 31 publications

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“…From Table 2 , the main methods used for the analysis of these pesticides involve the use of liquid chromatography combined with mass spectrometry (MS) and, in only one case, coupled with a fluorescence detector (FLD) [ 41 ].…”

Section: Analytical Methods For the Determination Of Glyphosate Glufo...mentioning

confidence: 99%

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Glyphosate and Glufosinate Residues in Honey and Other Hive Products

Rampazzo

Gazzotti

Zironi

et al. 2023

Foods

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Hive products have numerous beneficial properties; however, the hive’s health is affected by the surrounding environment. The widespread use of herbicides in agriculture, such as glyphosate and glufosinate, has raised alarm among consumers, beekeepers, and environmentalists due to their potential to harm bees and humans through the consumption of bee products. This review aims to provide a comprehensive overview of the presence of glyphosate, glufosinate, and their metabolites in hive products, collecting and comparing available data from peer-reviewed research and surveys conducted across several countries. Moreover, it analyzes and discusses the potential impacts of these substances on human and bee health, analytical aspects, and recent regulatory developments. The data has revealed that these substances can be present in the different matrices tested, but the concentrations found are usually lower than the maximum residue limits set. However, the use of different methodologies with non-uniform analytical performances, together with an incomplete search for regulated analytes, leads to heterogeneity and makes comparisons challenging. In addition to the completion of studies on the toxicology of herbicide active ingredients, further monitoring actions are necessary, harmonizing analytical methodologies and data management procedures.

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Section: Analytical Methods For the Determination Of Glyphosate Glufo...mentioning

confidence: 99%

“…Making these pesticides fluorescent is necessary when using fluorescence detectors. In the work of de Souza et al, this step was performed by post-column derivatization with o-phthalaldehyde (OPA) [ 41 ].…”

Section: Analytical Methods For the Determination Of Glyphosate Glufo...mentioning

confidence: 99%

Glyphosate and Glufosinate Residues in Honey and Other Hive Products

Rampazzo

Gazzotti

Zironi

et al. 2023

Foods

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“…To assess the impact of glyphosate on biofilm, bee gut bacterial strains capable of in vitro biofilm formation were cultured with varying glyphosate concentrations, ranging from 0.01 mM to 10 mM (1.69-1690 ppm). These concentrations simulate different environmental scenarios: minimal exposure, commonly found in water sources (62)(63)(64)(65); moderate concentrations detected in nectar (0.02-0.23 mM or 2.78-31.3 ppm) and pollen (0.63-4.57 mM or 87.2-629 ppm) collected from honey bees foraging on plants recently sprayed with glyphosate-based formulations in semi-field experiments (66); and higher, though still below worst-case, concentrations for topical exposure (67,68). The high concentrations were also used to counteract in vitro culture conditions with abundant nutrients, including some produced by the shikimate pathway, that could mask inhibition by glyphosate.…”

Section: Glyphosate Impacts Bee Gut Bacterial Growth and Biofilm Form...mentioning

confidence: 99%

Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria

Motta,

de Jong,

Gage

et al. 2024

Preprint

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Biofilm formation is a common adaptation enabling bacteria to thrive in various environments and to withstand external pressures. In the context of host-microbe interactions, biofilms play vital roles in establishing microbiomes associated with animals and plants and are used by opportunistic microbes to facilitate proliferation within hosts. Investigating biofilm dynamics, composition, and responses to environmental stressors is crucial for understanding microbial community assembly and biofilm regulation in health and disease. In this study, we explore the independent gut colonization and in vitro biofilm formation abilities of core members of the honey bee (Apis mellifera) gut microbiota. Additionally, we assess the impact of glyphosate, a widely used herbicide with antimicrobial properties, and a glyphosate-based formulation on growth and biofilm formation in bee gut symbionts as well as in other biofilm-forming bacteria associated with diverse animals and plants. Our results demonstrate that several strains of core bee gut bacterial species can independently colonize the bee gut, which probably depends on their ability to form biofilms. Furthermore, glyphosate exposure has varying effects on bacterial growth and biofilm formation. These findings imply specific impacts of environmental stressors on microbial biofilms with both ecological and host health-related implications.

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“…In Brazil, glyphosate residues were quantified in 40 honey samples from five states, of which six had glyphosate levels above the maximum residue limit (MRL) proposed by the European Union (EU) (0.05 μg g −1 ), and one sample showed AMPA at 0.10 μg g −1 (de Souza et al, 2020;Medina-Pastor et al, 2020). The Brazilian regions with the highest presence of glyphosate residues in honey were the same ones that recorded high losses of bee colonies and frequent use of HBG in agriculture, mainly in soybean cultivation (De Souza et al, 2020).…”

Section: Analytementioning

confidence: 99%

“…Even so, some substances are not detected, generating the false idea that they are not present and, therefore, are not associated with the weakening and loss of hives (Sánchez-Bayo et al, 2016). This is the case of glyphosate, the most widely used herbicide worldwide (Benbrook, 2016;Giesy et al, 2000), applied before and during the flowering of crops, which may result in residues in honey (De Souza et al, 2020;Karise et al, 2017;Rubio et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

Faita

Alves

Micke

et al. 2023

dataset

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Honey and other bee products may contain residues of different substances, including pesticides, which is considered a public health problem. In addition, they characterize risks to the health of Apis mellifera, which have been showing an increasing decline in their populations. There are many protocols for identifying pesticides in bee products which, in general, are complex matrices whose results of routine investigations in control laboratories are rarely disclosed. In this sense, the objective of the present study was to determine the presence of residues of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) in honey, as well as its effect on the strength of the hive of A. mellifera. Samples were collected from hives experimentally exposed to food containing a sublethal dose of Roundup® and conducted by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry (HILIC-MS/MS). The AMPA content was lower than the method’s detection limit in honey samples from hives that received food containing the herbicide. It was possible to quantify glyphosate one week after the last artificial feeding (R1 8.45 ± 1.09 µg g−1; R2 8.15 ± 2.14 µg g−1; R3 23.90 ± 2.95 µg g−1). In a hive sample fed for more than four weeks, glyphosate was present in lower concentrations (3.12 ± 0.89 µg g−1) with no detection of AMPA. From the analysis of the strength of the hives, we observed a decrease in the population of adult individuals and the brood area, the absence of a queen, and no construction of royal cells by the workers in the hives of the Roundup® treatment in comparison to the control group, in which the hives remained with queen size, high adult and brood population, and food stock. Although present, glyphosate did not undergo degradation in honey during the evaluated period. Thus, we could infer that the presence of Roundup® in bee feed may be present in honey, representing a risk to consumers’ health and economic damage to beekeepers. This is the first study that evaluated the effect of glyphosate residues on hive strength, contributing to the understanding of the Roundup® mode of action in different aspects that affect the survival of colonies under field conditions.

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Glyphosate and aminomethylphosphonic acid (AMPA) residues in Brazilian honey

Cited by 28 publications

References 31 publications

Glyphosate and Glufosinate Residues in Honey and Other Hive Products

Glyphosate and Glufosinate Residues in Honey and Other Hive Products

Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria

Glyphosate residue in honey and impacts on Africanized bee hives under field conditions

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