Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa

Ansari, Muhammad Tayyab; Rakha, Bushra Allah; Andrabi, S. M. H.; Ullah, Nemat; Iqbal, Razia; Holt, William V.; Akhter, Shamim

doi:10.1016/j.repbio.2012.10.001

Cited by 51 publications

(30 citation statements)

References 21 publications

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“…The mean value of motility obtained was higher than that of Kaka et al (2011) and Ansari et al (2014), each having a post thawing motility value of 43.25 ± 3.40% and 41.67 ± 2.90% in Kundhi buffalo. This difference was thought to be due to differences in buffalo breeds as the source of semen and genetic quality of each Bull.…”

Section: Evaluation Of Spermatozoa Buffalo Motilitycontrasting

confidence: 68%

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Mahendra

Samsudewa

Ondho

2018

J. Indonesian Trop. Anim. Agric.

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This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value.

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Section: Evaluation Of Spermatozoa Buffalo Motilitycontrasting

confidence: 68%

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Mahendra

Samsudewa

Ondho

2018

J. Indonesian Trop. Anim. Agric.

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“…The previous studies showed that the freeze-thaw process reduces the content of sperm glutathione in of boars [32], bulls [33] and goats [17]. And addition of glutathione to the freezing extenders improved the sperm quality [32, 34] though Marco-Jiménez et al (2006) [35] reported that GSH had no effect on rabbit sperm during cooling and freezing process. In this study, we further observed that the sperm glutathione decreased at each step, including cooling, equilibrium, freeze-thaw and post-thaw incubation.…”

Section: Discussionmentioning

confidence: 99%

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

et al. 2017

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The process of cryopreservation results in over-production of reactive oxygen species, which is extremely detrimental to spermatozoa. The aim of this study was to investigate whether addition of cysteine to freezing extender would facilitate the cryosurvival of rabbit spermatozoa, and if so, how cysteine protects spermatozoa from cryodamages. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of cysteine. The motility, intact acrosomes, membrane integrity, mitochondrial potentials, 8-hydroxyguanosine level and sperm-zona pellucida binding capacity were examined. Furthermore, glutathione peroxidase (GPx) activity, glutathione content (GSH), and level of reactive oxygen species (ROS) and hydrogen peroxide of spermatozoa were analyzed. The values of motility, intact acrosomes, membrane integrity, mitochondrial potentials and sperm-zona pellucida binding capacity of the frozen-thawed spermatozoa in the treatment of cysteine were significantly higher than those of the control. Addition of cysteine to extenders improved the GPx activity and GSH content of spermatozoa, while lowered the ROS, DNA oxidative alterations and lipid peroxidation level, which makes spermatozoa avoid ROS to attack DNA, the plasma membrane and mitochondria. In conclusion, cysteine protects spermatozoa against ROS-induced damages during cryopreservation and post-thaw incubation. Addition of cysteine is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry.

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“…Sperm DNA integrity was assessed by acridine orange assay (Ansari et al., ). The air‐dried smears of semen sample on glass slide were fixed overnight in Carnoy's solution (freshly prepared, methanol [Merck] and glacial acetic acid [Merck]; 3:1).…”

Section: Methodsmentioning

confidence: 99%

“…It is worth mentioning that the decision on the usability of any semen extender in routine artificial insemination programme is always based on the results of in vivo fertility rates of buffalo under field conditions (Akhter, Sajjad, Andrabi, Ullah, & Qayyum, ; Akhter, Ansari, et al., ; Akhter, Rakha, et al., ; Ansari, Rakha, Akhter, & Ashiq, ; Ansari et al., ). It was hypothesized that cryopreservation of buffalo semen in AndroMed ® extender may result in higher post‐thawed semen quality and in vivo fertility rate.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed^® extender; in vitro and in vivo evaluation

Ansari

Rakha

Akhter

2017

Reprod Domestic Animals

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The study was designed to evaluate AndroMed for the freezability and fertility of Nili-Ravi buffalo semen. Semen was collected from four adult Nili-Ravi buffalo (Bubalus bubalis) bulls for 3 weeks (replicate). Semen ejaculates from each buffalo bull were divided into three aliquots. One aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, DNA integrity and normal apical ridge. Remaining two aliquots were diluted (37°C; 50 × 10 spermatozoa/ml) in tris-citric egg yolk or AndroMed extender and cryopreserved in 0.5 ml French straws. After thawing, per cent post-thaw motility (47.9 ± 0.8, 49.2 ± 1.7), plasma membrane integrity (44.4 ± 1.2, 46.8 ± 1.8) and normal apical ridge (81.4 ± 0.3, 83.2 ± 0.3) were recorded similar (p > .05) in tris-citric egg yolk and AndroMed extender. Higher (p < .05) percentage of sperm livability (70.5 ± 1.4 and 64.4 ± 1.0), viability (67.5 ± 1.5 and 61.5 ± 0.6) and DNA integrity (97.0 ± 0.3 and 93.4 ± 0.21) were recorded in AndroMed compared to tris-citric egg yolk post-thaw. Values for all the aforementioned spermatozoal quality parameters were observed lower (p < .05) in frozen-thawed compared to fresh semen irrespective of the experimental extenders. Fertility rates of buffalo semen did not differ (p > .05) either cryopreserved in tris-citric egg yolk or AndroMed extender (45.5% vs. 49%). It is concluded that AndroMed is capable in protecting the buffalo bull sperm during freeze-thawing process and can be adopted safely for routine use replacing the tris-citric egg yolk extender in artificial insemination programme.

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Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa

Cited by 51 publications

References 21 publications

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed^® extender; in vitro and in vivo evaluation

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Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa

Cited by 51 publications

References 21 publications

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation

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Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed^® extender; in vitro and in vivo evaluation