Cryopreservation of Nili‐Ravi buffalo (<i>Bubalus bubalis</i>) semen in AndroMed<sup>®</sup> extender; in vitro and in vivo evaluation

Ansari, .; Rakha, Bushra Allah; Akhter, Shamim

doi:10.1111/rda.13008

Cited by 15 publications

(24 citation statements)

References 31 publications

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“…The higher fertility rates were recorded with extender having 15% pigeon egg yolk compared to 20% chicken egg yolk. The real test to evaluate a semen sample is to check the fertility rate after its in vivo insemination in a routine artificial insemination programme under field conditions [46][47][48]. The higher fertility rate observed with 15% pigeon egg yolk observed in the present study are in line with the higher sperm quality viz; motility, plasma membrane integrity, livability, viability, chromatin integrity and extracellular enzyme leakage suggesting that all the sperm quality parameters used to assess the semen quality do have essential role in the fertilization process [49].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

et al. 2018

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“…Rataan abnormalitas ini berbeda dengan Leite et al (2010) pada kerbau lumpur. Hal ini kemungkinan disebabkan oleh meningkatnya persentase abnormalitas semen kerbau dipengaruhi oleh faktor pengelolahan bahan pengencer dan pendinginan (Ansari et al, 2017). Selain itu pengencer andromed lebih mampu menjaga spermatozoa dari kerusakan akibat suhu rendah dan pengencer andromed juga merupakan buffer yang dapat menekan penurunan kualitas spermatozoa selama proses pendinginan, pembekuan dan thawing (Akhter et al, 2010).…”

Section: Kualitas Semen Pada Waktu Ekuilibrasi Yang Berbedaunclassified

Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST

Yendraliza¹,

Yuliana²,

Rodiallah³

et al. 2019

J. Agripet

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ABSTRAK. Penelitian ini bertujuan untuk mengetahui waktu ekuilibrasi yang terbaik semen kerbau menggunakan pengencer andromed dan mengetahui nilai membran plasma utuh semen kerbau dalam larutan hipoosmotik pada waktu pemeraman yang berbeda. Penelitian ini terdiri dari dua tahap; Tahap pertama menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perlakuan terdiri dari waktu ekuilibrasi 3 jam, 4 jam dan 5 jam. Parameter yang diukur adalah motilitas, sperma hidup dan abnormalitas. Penelitian tahap kedua menggunakan Rancangan Acak Lengkap (RAL) dua faktor dengan 3 perlakuan dan 5 ulangan. Faktor pertama adalah waktu ekuilibrasi yang terdiri dari 3 jam, 4 jam dan 5 jam. Faktor kedua adalah waktu pencampuran semen dengan larutan hipoosmotik yang terdiri 15 menit, 30 menit dan 45 menit. pengencer yang digunakan Andromed(R) (Minitue Germany, 13503/0200). Parameter yang diukur adalah persentase membran plasma utuh semen kerbau. Hasil penelitian yang didapatkan bahwa waktu ekuilibrasi terbaik adalah 4 jam dan 5 jam dengan nilai motilitas 62,8-63,7%, sperma hidup 66,7-66,8% dan persentase abnormalitas 10,5-11,2%. Waktu HOS test yang terbaik adalah 45 menit dan waktu ekuilibrasi 5 jam dengan nilai MPU (64,7 : 57,7%). Kesimpulan penelitian ini adalah waktu ekuilibrasi semen kerbau menggunakan pengencer andromed adalah 5 jam dengan waktu pemeraman dalam larutan hipoosmotik adalah 45 menit. (Semen quality of buffalo at different equilibration and incubation period in hipoosmotic solution of swelling TEST) ABSTRACT The aimed of this study was to find out the best equilibration time of buffalo semen used andromed diluents and the optimal time to test the integrity of plasma membrane of buffalo semen using a hypoosmotic swelling test (HOS test). This study consists of two stages; the first step was used a Completely Randomized Design (CRD) with 3 treatments and 5 replications. The treatments were equilibration time of 3 hours, 4 hours and 5 hours. The parameters measured were motility, live sperm, and abnormalities. The second stage of the study used a two-factor Completely Randomized Design (CRD) with 3 treatments and 5 replications. The first factor was the equilibration time of 3 hours, 4 hours and 5 hours. The second factor was the time of semen mixing with a hypoosmotic solution of 15 minutes, 30 minutes and 45 minutes. diluents used by Andromed(R) (Minitube Germany, 13503/0200). The parameters measured were the percentage of the integrity of the plasma membrane of buffalo semen. The results showed that the best equilibration time was 4 hours and 5 hours with motility values 62.8-63.7%, live sperm 66.7-66.8% and percentage abnormalities 10.5-11.2%. The best HOS test time is 45 minutes and the equilibration time is 5 hours with MPU value (64.7: 57.7%). The conclusion of the research is that the equilibration time of buffalo semen using andromed diluents was 5 hours with the mixing time in the hypoosmotic solution being 45 minutes.

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“…Furthermore, the efficiency of the soy lecithin-based extender BioXcell and Triladyl has been shown to be comparable [13], but BioXcell resulted in lowest total bull sperm motility, as well as plasma membrane and acrosome integrity, when compared to the egg yolk-supplied diluent Botu-Bov [34]. In buffaloes, OPTIXcell resulted in providing a better semen cryoprotection than soy lecithin- or egg yolk-based extenders [35,36], while AndroMed resulted in similar post-thawing sperm motility but better vitality and DNA integrity than egg yolk-based diluents [22]. Collectively the results of previous studies in cows and buffaloes indicate that soy lecithin or liposome-based extenders have a similar or better cryoprotective efficiency compared with egg-yolk based extenders.…”

Section: Discussionmentioning

confidence: 99%

“…The most commonly used substitutes are soy lecithin, low-density lipoprotein (LDL), and liposomes [5,20]. Promising results have been published regarding the use of animal-free egg substitutes for semen cryopreservation [5,20,21,22]. AndroMed and OPTIXcell are the most common bull extenders that are supplied with egg yolk replacer.…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Commercial Egg Yolk-Free and Egg Yolk-Supplemented Tris-Based Extenders for Dromedary Camel Semen Cryopreservation

Swelum

Saadeldin

Al-Mutary

et al. 2019

Animals

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Simple SummaryThis study compared the efficiency of commercial egg yolk-free (AndroMed, OPTIXcell) and egg yolk-supplemented (Triladyl, Steridyl) Tris-based extenders for semen cryopreservation in dromedary camels. The camel-specific extender SHOTOR was used for reference. SHOTOR, Triladyl, Steridyl, AndroMed, and OPTIXcell can all be used for camel semen cryopreservation; however, SHOTOR and Triladyl provide the best post-thawing sperm quality.AbstractThis study compared the efficiency of commercial egg yolk-free (AndroMed, OPTIXcell) and egg yolk-supplemented (Triladyl, Steridyl) Tris-based extenders for semen cryopreservation in seven adult dromedary camels. The camel-specific extender SHOTOR was used as control. The collected semen samples were evaluated and diluted with SHOTOR, Triladyl, Steridyl, AndroMed, or OPTIXcell. The diluted semen was gradually cooled and equilibrated for two hours before liquid nitrogen freezing. Semen was evaluated prior to freezing and after freeze-thawing cycles for motility, kinetics, vitality, abnormality, plasma membrane integrity, and DNA fragmentation using computer-assisted sperm analysis. In pre-freezing evaluation, progressive sperm motility was higher in SHOTOR-diluted samples (21.54 ± 1.83) than in samples diluted with Steridyl, OPTIXcell, or AndroMed (15.76 ± 1.80, 17.43 ± 1.10, and 13.27 ± 1.07, respectively). Moreover, Triladyl and SHOTOR resulted in significantly (p < 0.05) better sperm vitality and DNA integrity than all other diluents, but Triladyl resulted in a significantly (p < 0.05) better plasma membrane integrity (87.77 ± 0.31) than SHOTOR (85.48 ± 0.58). In the post-thawing evaluation, Triladyl led to significantly (p < 0.05) higher sperm motility (38.63 ± 0.81%; p < 0.05) when compared to SHOTOR, Steridyl or AndroMed (35.09 ± 1.341%, 34.4 ± 0.84%, and 31.99 ± 1.48%, respectively), with OPTIXcell being the least efficient (28.39 ± 0.86%). Progressive sperm motility was the highest when using Triladyl. Post-thawing curvilinear, straight line and average path sperm velocities were highest with Triladyl and lowest with AndroMed. Triladyl led to the highest linearity coefficient and straightness sperm coefficient, while SHOTOR to the highest DNA and plasma membrane integrity. OPTIXcell and AndroMed resulted in poor post-thawing sperm vitality, while Steridyl was less efficient than Triladyl. The highest rate of sperm abnormalities was recorded with OPTIXcell and the lowest with SHOTOR or Triladyl. In conclusion, SHOTOR, Triladyl, Steridyl, AndroMed, and OPTIXcell can all be used for camel semen cryopreservation; however, SHOTOR and Triladyl provided the best post-thawing sperm quality. Based on our findings, Triladyl is the best commercially available extender for dromedary camel semen cryopreservation to date.

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Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed^® extender; in vitro and in vivo evaluation

Cited by 15 publications

References 31 publications

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST

Efficiency of Commercial Egg Yolk-Free and Egg Yolk-Supplemented Tris-Based Extenders for Dromedary Camel Semen Cryopreservation

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Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation

Cited by 15 publications

References 31 publications

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST

Efficiency of Commercial Egg Yolk-Free and Egg Yolk-Supplemented Tris-Based Extenders for Dromedary Camel Semen Cryopreservation

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Product

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Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed^® extender; in vitro and in vivo evaluation