Gibberellin recovers seed germination in rice with impaired brassinosteroid signalling

Li, Qianfeng; Zhou, Yu; Xiong, Min; Ren, Xinyu; Han, Lei; Wang, Jin-Dong; Zhang, Changquan; Fan, Xiaolei

doi:10.1016/j.plantsci.2020.110435

Cited by 53 publications

(33 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Brassionsteroid (BR) is a group of plant-speci c polyhydroxylated steroidal hormones that regulate cell elongation, seed germination, and so on Li et al 2020). In our studies, B8AQM6 joined in the brassinosteroidmediated signaling pathway and was upregulated in the autotetraploid (Table 4).…”

Section: Discussionmentioning

confidence: 79%

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

Xian

Long

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Autotetraploid rice, which is developed through chromosome set doubling using diploid rice, produces high-quality kernels that are rich in storage proteins. However, little information is available about the content of different proteins in autotetraploid rice and their proteomic analysis. Results: The dynamic changes in four storage proteins, albumin, globulin, prolamin, and glutelin, were analyzed in the endosperm of autotetraploid rice (AJNT-4x) and in that of its diploid counterpart (AJNT-2x) for comparison. The contents of the four proteins were all higher during endosperm development in AJNT-4x than in AJNT-2x, but their change and composition were almost the same in the two materials. Then, iTRAQ was employed to analyze the glutelin profiles of AJNT-4x and AJNT-2x at 10 DAF, 15 DAF, and 20 DAF. A total of 1,326 proteins were identified in AJNT-4x and AJNT-2 x using high-throughput LC-MS/MS. Among the 1,326 identified proteins, there were 362 DEPs in AJNT-4x compared with those in AJNT-2x and 372 DEPs between different development stages in AJNT-4x. Eight important upregulated proteins were identified by qRT-PCR, including B8AM24, B8ARJ0, B8AQM6, A2ZCE6, and Q40689. Among them, B8AM24 and B8ARJ0 were related to the lysine biosynthesis process. GO enrichment analysis revealed that the critical functions of DEPs exhibited little overlap between the 10, 15, and 20 DAF groups. Endosperm glutelin accumulation was regulated mainly by different DEPs during the late stage, and 15 DAF was a critical regulating point for glutelin accumulation. KEGG pathway analysis showed that ribosomal proteins were significantly higher in AJNT-4x than in AJNT-2x at 10 DAF, and the protein processing, biosynthesis, and metabolism of amino acids were higher and more active in AJNT-4x at 15 DAF, while the peroxisome was richer in AJNT-4x at 20 DAF. The PPI network showed that ribosomal proteins gradually decreased with increasing endosperm development. Conclusions: These results provide new insights into dynamic glutelin expression differences during endosperm development in autotetraploid rice, which will help in developing rice cultivars with increased yield and improved grain nutritional quality.

show abstract

Section: Discussionmentioning

confidence: 79%

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

Xian

Long

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Correlation analysis of the gene expression between qRT-PCR and RNA-seq data indicated that the values were consistent (correlation coefficient R 2 = 0.74), confirming the accuracy of the RNA-seq data ( Figure 6B). Because the expression of up to six LEA genes was upregulated by ABA treatment 286 and in osbzip09-2, LEA25 was selected as a representative gene for further analysis, and 287 was also identified by previous proteomic study of rice seed germination [25]. Moreover, 288 LOX2, which was transcriptionally downregulated in this study and is reported to pro-289…”

Section: Expression Analysis and Validation Of The Representative Tarmentioning

confidence: 89%

“…LEAs are often considered to be ABA-responsive genes that mediate plant adaptation to various stresses, such as drought stress and antioxidant stress [57][58][59]. Recently, two reports have revealed that LEAs are also involved in the regulation of rice seed germination: the germination of OsLEA5-RNAi transgenic rice seeds was less sensitive to ABA treatment [60], and the mutation of OsLEA33 promoted post-germination growth of rice [25]. These studies suggest that OsLEAs play positive roles in plant stress responses and negative roles in rice seed germination.…”

Section: Discussionmentioning

confidence: 99%

“…Because the expression of up to six LEA genes was upregulated by ABA treatment and in osbzip09-2, LEA25 was selected as a representative gene for further analysis, and was also identified by previous proteomic study of rice seed germination [25]. Moreover, LOX2, which was transcriptionally downregulated in this study and is reported to promote germination [26], was also selected for subsequent assay.…”

Section: Expression Analysis Of the Representative Target Genes Regulmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.

show abstract

“…They demonstrated that BRs promoted an increased gravitropic response in Arabidopsis roots at low concentration of IAA and had reduced activity at high levels of IAA. Li et al [146] highlighted the role of brassinosteroid (BR) and redox signal hydrogen peroxide in breakdown of starch which is the major storage carbohydrate in plants. They reported that the brassinosteroid and redox signal hydrogen peroxide (H 2 O 2 ) induce the breakdown of starch in guard cells, which promotes stomatal opening.…”

Section: Role Of Brassinosteroids In Plant Physiology and Biochemistrymentioning

confidence: 99%

Brassinosteroids: A Multifunctional Phytohormone of Plant Development and Stress Responses

Praveena¹,

Dash²,

Behera

et al. 2020

CJAST

View full text Add to dashboard Cite

Brassinosteroids (BRs) act as immune-modulators for generating plant growth and development. It regulated either activation or suppression of various key enzymatic reactions, activation of synthesis of protein, and generation of various defense modulating compounds for the plant kingdom. BRs play a vital role in regulating cellular differentiation, pollen development, fruit ripening, and quality seed formation. BRs regulates the various physiological process including root growth during nutrient deficiency such as nitrogen, phosphorus, boron and tends to signal the nutrient distribution in the rhizosphere level for better growth and high yield in crop plants. This review highlighted the role of BRs in plant growth and development and stress response, understanding the BR pathway, the molecular mechanism of BR signaling in various tissues, crosstalk between BRs and other phytohormones, gene involves in the brassinosteroids signaling pathway, biosynthesis and role of BRs in biomass production and crop yield.

show abstract

Gibberellin recovers seed germination in rice with impaired brassinosteroid signalling

Cited by 53 publications

References 98 publications

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

iTRAQ-Based Quantitative Glutelin Proteomic Analysis Reveals Differentially Expressed Proteins in the Physiological Metabolism Process during Endosperm Development and their Impacts on Yield and Quality in Autotetraploid Rice

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Brassinosteroids: A Multifunctional Phytohormone of Plant Development and Stress Responses

Contact Info

Product

Resources

About