Bioinformatics is the new branch of science which deals with the acquisition, storage, analysis and dissemination of biological data with the help of computer science and information technology. It has the enormous ability to analyze a vast quantity of biological data quickly and cost-effectively. In the past decades, enormous sequence information has been generated due to the advances in DNA and protein sequencing techniques. Estimating similarities between biological sequences is becoming necessary to obtain hidden information present within the sequence and to trace evolutionary relationship exist within the sequences. This sequence comparison can be achieved by basic local alignment search tool (BLAST). So BLAST has become a fundamental tools of life science research. Hence it is essential to know how to do sequence comparison using BLAST and how to accurately interpret the BLAST output data. The present article aims to familiarize the biologists and researchers with different BLAST programs and their use in research program.

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Brassinosteroids (BRs) act as immune-modulators for generating plant growth and development. It regulated either activation or suppression of various key enzymatic reactions, activation of synthesis of protein, and generation of various defense modulating compounds for the plant kingdom. BRs play a vital role in regulating cellular differentiation, pollen development, fruit ripening, and quality seed formation.   BRs regulates the various physiological process including root growth during nutrient deficiency such as nitrogen, phosphorus, boron and tends to signal the nutrient distribution in the rhizosphere level for better growth and high yield in crop plants. This review highlighted the role of BRs in plant growth and development and stress response, understanding the BR pathway, the molecular mechanism of BR signaling in various tissues, crosstalk between BRs and other phytohormones, gene involves in the brassinosteroids signaling pathway, biosynthesis and  role of  BRs  in  biomass production and crop yield.

From the evolution of the mankind, Turmeric has been used in conventional medication. India is in lead for producing, marketing and exporting the Turmeric and its value added products. Curcuma longa (Turmeric) is an Indian rhizomatous medicinal herb from the Zingiberaceae family that is common and widely available across the globe. The components of Turmeric are curcumin, demethoxycurcumin and bisdemethoxycurcumin and these are collectively known as curcuminoids. Curcumin, the active ingredient of Turmeric is generally investigated by the scientific community for its wide range of antioxidant activity, anti-Inflammatory properties and anti-cancer activity, anti-metabolic syndrome activities, neuroprotective activity, antimicrobial effects, anti-arthritis effects, anti-viral effects, anti-asthma and anti-diabetic effects, anti-obesity, cardio and liver toxicity protection activity, anti-depression How to cite this paper:

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COVID-19, the infectious pandemic disease is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This deadly disease was unknown before its catastrophic outbreak of the infection in Wuhan city of China, in December 2019. The pandemic situation has increased the demand of rapid enhancement of the in-vitro diagnostic assays which would enable the mass screening and testing. Several molecular and serological diagnostics assays such as direct viral antigen tests, nucleic acid amplification tests and serological tests were developed. Nucleic acid tests such as RT-PCR. TrueNAT, Feluda Test, loop-mediated isothermal amplification (LAMP) etc. detect the presence of RNA virus in the nasal or throat swab or from saliva. Antigen tests detect the presence of a virus as the antigen, which is a surface protein. Antibody tests such as enzyme-linked immunosorbent assays (ELISA), lateral flow assays (LFA), chemiluminescence assays (CLIA) etc. detect the presence of antibodies generated against SARS-CoV-2 in the blood samples.

Background: Mungbean [Vigna radiata (L.) Wilczek] is a self-pollinated diploid grain legume (2n=2x=22) crop and has a genome size of 560 Mb. The present study was concentrate to portray the nature and extent of genotypic variation exists among mungbean collections for a range of traits of potential agronomic and adaptive interests. Many diseases affect mungbean, causes the major constraint in the increasing production among which Powdery mildew disease caused by Erisyphepolygoni is economically significant because it reduces photosynthetic activity and physiological changes which results in 20-40 per cent reduction in yield. Some gene-specific marker were analysed and found associated with powdery mildew resistance in mungbean genotypes.Methods: The present investigation was carried out to evaluate thirty-one mungbean genotypes (including four checks) collected from the different parts of India. The genotypes were sown in an incomplete augmented bock design along with four checks varieties. viz. ‘Kamdev’, ‘OBBGG-52’, ‘IPM-02-14’ and ‘IPM-02-3’. All the mungbean genotypes were evaluated for different phenotypic traits and their tolerance to powdery mildew disease at two cropping seasons as well as at two different locations in Odisha. In this investigation, seven molecular markers viz., VrCSSTS1, VrCSSTS2, VrCSSSR3, CEDG191, MB-SSR238, CEDG166, CEDG282 were analysed.Result: SSR marker such as VrCSSSR and VrCSSTS linked with powdery mildew resistance gene were tested in different genotypes with known powdery mildew reaction and the results showed a consistent association of the marker in all the powdery mildew resistant genotypes and absent in all the powdery mildew susceptible genotypes. The results confirmed the validation of these markers with the powdery mildew resistance gene in different genetic backgrounds. Similarly, CEDG191, CEDG166, CEDG282 markers, reported to be linked to powdery mildew resistance, amplified the respective marker fragment of 100 to 300 bp in mungbean genotypes and were polymorphic. The above PCR-based and locus-specific markers could be employed for marker-assisted breeding (MAB) program as well as genotype conservation. These linked markers will boost the efficiency and precision of powdery mildew resistance breeding in mungbean.