Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu, Chen; Wang, Chu-Xin; Lu, Chen-Ya; Wang, Jin-Dong; Zhou, Yu; Xiong, Min; Zhang, Changquan; Liu, Qiaoquan

doi:10.3390/ijms22041661

Cited by 13 publications

(12 citation statements)

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“…For instance, in a recent study, we generated two mutant rice lines by editing the gene that encodes the OsbZIP transcription factor OsbZIP09 using the CRISPR/Cas9 system. Our results indicate that this transcription factor is involved in regulation of seed germination through its attenuation of the ABA pathway [24]. Furthermore, it is reasonable to assume that, like other OsbZIPs (e.g., OsbZIP58), OsbZIP09 also plays an import role in regulating grain appearance as well as starch composition and morphology.…”

Section: Introductionmentioning

confidence: 69%

“…Interestingly, OsbZIP58 was also reported to inhibited the expression of some starch-hydrolyzing α-amylase genes, which were considered as important positive effectors of rice seed germination [31]. In fact, our previous studies have shown that OsbZIP09 functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies [24,32]. Additionally, we found Grain chalkiness is one of the most important factors affecting rice appearance, milling, and eating quality [29,30].…”

Section: Analysis Of Grain Quality Profilesmentioning

confidence: 99%

“…It is thought that the minimum starch chain length required to form double helices is 10 monomeric units [47], and amylopectin short-branch chains decrease packing efficiency in the starch, lowering crystallinity and thus decreasing the gelatinization temperature [15,45]. Accordingly, the increased abundance of short amylopectin chains (DP 6-12) and decreased abundance of intermediate chains (DP [13][14][15][16][17][18][19][20][21][22][23][24] in the OsbZIP09 mutant rice is the main reason for the reduced crystallinity of the starch. In order to investigate the effects of OsbZIP09 mutation on short-range starch structure (helical order) near granule surfaces, ATR-FTIR was performed.…”

Section: Starch Crystalline Structurementioning

confidence: 99%

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Grain Quality and Starch Physicochemical Properties of Chalky Rice Mutant

Wang

Zhu

et al. 2021

Agronomy

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Rice mutants with altered starch components and properties are important genetic resources for grain quality and starch structure analysis. Accordingly, in the present study, two mutants of the transcription factor OsbZIP09 were generated (osbzip09a and osbzip09b), and the rice grain quality and physicochemical starch properties of the mutant and wild-type lines were compared. The OsbZIP09 mutants exhibit a chalky grain owing to loosely packed, small, spherical starch granules in the ventral region of the endosperm. Furthermore, grain-quality profile analysis showed that OsbZIP09 deficiency leads to increased apparent amylose content but decreased gel consistency. Structural analysis of the mutant starches revealed that the mutant rice lines contain more amylopectin short chains and fewer intermediate chains, leading to lower crystallinity and lower gelatinization properties than those of the wild-type rice. Moreover, the OsbZIP09 mutants rice presented a significantly higher pasting curve and corresponding parameters than the wild-type rice. The results from this work strongly indicate that the transcription factor OsbZIP09 plays an important role in rice grain quality and starch fine structure modification, and extend our understanding of starch biosynthesis in rice endosperm.

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Section: Introductionmentioning

confidence: 69%

Section: Analysis Of Grain Quality Profilesmentioning

confidence: 99%

Section: Starch Crystalline Structurementioning

confidence: 99%

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Grain Quality and Starch Physicochemical Properties of Chalky Rice Mutant

Wang

Zhu

et al. 2021

Agronomy

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“…High-quality genome resources enabled the detection of pre-harvest sprouting-related genes via GWASs and genome-wide identification approaches. Zhu et al [ 121 ] pinpointed the role of the bZIP transcription factor OsbZIP09 , whose expression is induced by ABA. The mutation of this gene inhibited pre-harvest sprouting in rice.…”

Section: Omics Approaches For Pre-harvest Sprouting In Ricementioning

confidence: 99%

Seed Dormancy and Pre-Harvest Sprouting in Rice—An Updated Overview

Sohn

Subramanian

Thamilarasan

et al. 2021

IJMS

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Pre-harvest sprouting is a critical phenomenon involving the germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. As it results in reduced grain yield and quality, it is a common problem for the farmers who have cultivated the rice and wheat across the globe. Crop yields need to be steadily increased to improve the people’s ability to adapt to risks as the world’s population grows and natural disasters become more frequent. To improve the quality of grain and to avoid pre-harvest sprouting, a clear understanding of the crops should be known with the use of molecular omics approaches. Meanwhile, pre-harvest sprouting is a complicated phenomenon, especially in rice, and physiological, hormonal, and genetic changes should be monitored, which can be modified by high-throughput metabolic engineering techniques. The integration of these data allows the creation of tailored breeding lines suitable for various demands and regions, and it is crucial for increasing the crop yields and economic benefits. In this review, we have provided an overview of seed dormancy and its regulation, the major causes of pre-harvest sprouting, and also unraveled the novel avenues to battle pre-harvest sprouting in cereals with special reference to rice using genomics and transcriptomic approaches.

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“…Being able to suppress PHS without re-introducing seed dormancy has become a great challenge to crop improvement. Zhu et al have identified an abscisic acid (ABA)-regulated basic leucine zipper-encoding gene from rice ( OsbZIP09 ) that is involved in PHS [ 10 ]. They found that the mutation of OsbZIP09 could partially suppress PHS with only minor delay in seed germination [ 10 ].…”

mentioning

confidence: 99%