GhHUB2, a ubiquitin ligase, is involved in cotton fiber development via the ubiquitin–26S proteasome pathway

Feng, Hao; Li, Xin; Chen, Hong; Zhang, Chaojun; Liu, Ji; Wang, Tao; Zhang, Xueyan; Dong, Jiangli

doi:10.1093/jxb/ery269

Cited by 31 publications

(23 citation statements)

References 83 publications

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“…To understand how AtHUB2 overexpression improves transgenic cotton drought tolerance, we performed a yeast two‐hybrid assay to screen a cotton whole‐growth‐period cDNA library using AtHUB2‐pDEST32 (At1g55250.2) as bait, and a series of candidate genes were identified (Table S2). The cotton histone GhH2B1, which was previously identified as candidate GhHUB2 interact protein (Feng et al ., ), was included in the group of 17 positive clones. Multiple sequence alignment and phylogenetic tree analysis showed that the GhH2B1 C‐terminus and key functional amino acid sites are conserved (Figure S6) (Hwang et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…The multiple sequence alignment results showed that these two GhHUB2s share 98.6% nucleotide identity and 98.4% amino acid sequence identity and that both contain a conserved C3HC4 RING finger domain in the C-terminal region. Therefore, the 272 bp region from the 3 0 end, which included the RING domain shared by both GhHUB2s, was chosen for RNAi (Feng et al, 2018). Three T4 generation plants with significantly down-regulated expression of GhHUB2 were used to perform drought tolerance assays (401-34, 401-57 and 401-52).…”

Section: Ghhub2-specific Rnai Reduces Drought Tolerance In Transgenicmentioning

confidence: 99%

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An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

Chen

Feng

Zhang

et al. 2018

Plant Biotechnology Journal

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The HUB2 gene encoding histone H2B monoubiquitination E3 ligase is involved in seed dormancy, flowering timing, defence response and salt stress regulation in Arabidopsis thaliana. In this study, we used the cauliflower mosaic virus (CaMV) 35S promoter to drive AtHUB2 overexpression in cotton and found that it can significantly improve the agricultural traits of transgenic cotton plants under drought stress conditions, including increasing the fruit branch number, boll number, and boll-setting rate and decreasing the boll abscission rate. In addition, survival and soluble sugar, proline and leaf relative water contents were increased in transgenic cotton plants after drought stress treatment. In contrast, RNAi knockdown of GhHUB2 genes reduced the drought resistance of transgenic cotton plants. AtHUB2 overexpression increased the global H2B monoubiquitination (H2Bub1) level through a direct interaction with GhH2B1 and up-regulated the expression of drought-related genes in transgenic cotton plants. Furthermore, we found a significant increase in H3K4me3 at the DREB locus in transgenic cotton, although no change in H3K4me3 was identified at the global level. These results demonstrated that AtHUB2 overexpression changed H2Bub1 and H3K4me3 levels at the GhDREB chromatin locus, leading the GhDREB gene to respond quickly to drought stress to improve transgenic cotton drought resistance, but had no influence on transgenic cotton development under normal growth conditions. Our findings also provide a useful route for breeding drought-resistant transgenic plants.

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Section: Resultsmentioning

confidence: 99%

Section: Ghhub2-specific Rnai Reduces Drought Tolerance In Transgenicmentioning

confidence: 99%

An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

Chen

Feng

Zhang

et al. 2018

Plant Biotechnology Journal

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“…HISTONE MONOUBIQUITINATION2 (HUB2) encoding histone H2B monoubiquitination E3 ligase was shown to have a role in fibre elongation and SCW deposition in transgenic cotton. Moreover, a key transcriptional repressor, GhKNL1, was ubiquitinated and degraded directly via the ubiquitin‐26S proteasome associated with GhHUB2, which promoted downstream gene expression, fibre elongation and SCW deposition (Feng et al ., ; Figure 4).…”

Section: The Mechanisms Of Epigenetic Modifications In Cotton Fibre Dmentioning

confidence: 96%

Updates on molecular mechanisms in the development of branched trichome in Arabidopsis and nonbranched in cotton

Wang

Yang

2019

Plant Biotechnology Journal

108

112

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Summary Trichomes are specialized epidermal cells and a vital plant organ that protect plants from various harms and provide valuable resources for plant development and use. Some key genes related to trichomes have been identified in the model plant Arabidopsis thaliana through glabrous mutants and gene cloning, and the hub MYB ‐ bHLH ‐ WD 40, consisting of several factors including GLABRA 1 ( GL 1), GL 3, TRANSPARENT TESTA GLABRA 1 ( TTG 1), and ENHANCER OF GLABRA 3 ( EGL 3), has been established. Subsequently, some upstream transcription factors, phytohormones and epigenetic modification factors have also been studied in depth. In cotton, a very important fibre and oil crop globally, in addition to the key MYB ‐like factors, more important regulators and potential molecular mechanisms (e.g. epigenetic modifiers, distinct metabolic pathways) are being exploited during different fibre developmental stages. This occurs due to increased cotton research, resulting in the discovery of more complex regulation mechanisms from the allotetraploid genome of cotton. In addition, some conservative as well as specific mediators are involved in trichome development in other species. This study summarizes molecular mechanisms in trichome development and provides a detailed comparison of the similarities and differences between Arabidopsis and cotton, analyses the possible reasons for the discrepancy in identification of regulators, and raises future questions and foci for understanding trichome development in more detail.

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“…Besides their role in chromatin modifications, E3 ubiquitin ligases, such as HUB2, have been implicated in ubiquitin-dependent proteolysis. A recent study in cotton proposed that the functional homologue of AtHUB2, GhHUB2, is involved in protein degradation via the ubiquitin–26S proteasome pathway ( Feng et al , 2018 ). We cannot therefore rule out that the function of HUB2 in lignification is somehow related to proteolysis.…”

Section: Discussionmentioning

confidence: 99%

The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels

Zhang

Sztojka

Seyfferth

et al. 2020

Journal of Experimental Botany

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PIRIN2 (PRN2) was earlier reported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis thaliana. In the present study, we report yeast two-hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been previously implicated in several plant developmental processes, but not in lignification. Interaction between PRN2 and HUB2 was verified by β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted the deposition of S-type lignin in the secondary cell walls of both stem and hypocotyl tissues, as analysed by pyrolysis-GC/MS. Chemical fingerprinting of individual xylem vessel cell walls by Raman and Fourier transform infrared microspectroscopy supported the function of HUB2 in lignin deposition. These results, together with a genetic analysis of the hub2 prn2 double mutant, support the antagonistic function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome analyses indicated the opposite regulation of the S-type lignin biosynthetic gene FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying mechanism. PRN2 and HUB2 promoter activities co-localized in cells neighbouring the xylem vessel elements, suggesting that the S-type lignin-promoting function of HUB2 is antagonized by PRN2 for the benefit of the guaiacyl (G)-type lignin enrichment of the neighbouring xylem vessel elements.

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GhHUB2, a ubiquitin ligase, is involved in cotton fiber development via the ubiquitin–26S proteasome pathway

Abstract: GhHUB2, a ubiquitin ligase, is involved in cotton fiber elongation and secondary cell wall deposition by degrading GhKNL1 via the ubiquitin–26S proteasome pathway, and thus demonstrates a novel function of HUB2 in plants.

Cited by 31 publications

References 83 publications

An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

Updates on molecular mechanisms in the development of branched trichome in Arabidopsis and nonbranched in cotton

The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels

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