An <i>Arabidopsis</i> E3 ligase <i><scp>HUB</scp>2</i> increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

Chen, Hong; Feng, Hao; Zhang, Xueyan; Zhang, Chaojun; Wang, Tao; Dong, Jiangli

doi:10.1111/pbi.12998

Cited by 54 publications

(59 citation statements)

References 49 publications

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“…Interestingly, the H2Bub1 level was also induced in Arabidopsis under drought stress treatments (Supplemental Figure 10). Consistent with this, a newly published work pointed to substantial roles for AtHUB2 and GhHUB2 (a homolog of AtHUB2 in cotton) in drought stress response (Chen et al, 2018). The overall data suggest that H2Bub1 may function as a general epigenetic regulatory mechanism in response to drought stress.…”

Section: Discussionsupporting

confidence: 74%

Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

Tang

et al. 2019

Molecular Plant

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Histone H2B monoubiquitination (H2Bub1) plays important roles in several physiological and developmental processes, but its roles in the regulation of plant stress responses remain elusive. Here, we report that H2Bub1 is crucially involved in abscisic acid (ABA) signaling and drought response in rice. We found that rice HISTONE MONOUBIQUITINATION2 (OsHUB2), an E3 ligase for H2Bub1, interacted with OsbZIP46, a key transcription factor regulating ABA signaling and drought response in rice. Genetic analyses suggest that OsHUB2, upregulated by drought and ABA, positively modulates ABA sensitivity and drought resistance. The H2Bub1 levels were increased in the target genes of OsbZIP46 under the drought stress and ABA treatments, which were positively correlated with their increased expression levels. Interestingly, MODD, a reported suppressor of ABA signaling and drought resistance by mediating OsbZIP46 deactivation and degradation, could reduce the H2Bub1 levels in the target genes of OsbZIP46 by recruiting a putative deubiquitinase OsOTLD1. Suppression of OsOTLD1 in vivo resulted in increased H2Bub1 levels and expression of OsbZIP46 target genes. Collectively, these findings established an elaborate mechanism of histone monoubiquitination in the fine-turning of ABA signaling and drought response by balancing H2Bub1 deposition and removal.

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Section: Discussionsupporting

confidence: 74%

Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

Tang

et al. 2019

Molecular Plant

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“…In Arabidopsis , E3 ligases (HUB1 and HUB2, HISTONE MONOUBIQUITINATION1 and ‐2) and E2 conjugated enzymes (UBC1 and UBC2, UBIQUITIN CARRIER PROTEIN1 and ‐2) are responsible for ubiquitination of histone H2B. Histone H2B monoubiquitination (H2Bub1) regulates multiple developmental processes and is involved in biotic and abiotic stress responses (Fleury et al , 2007; Cao et al , 2008; Dhawan et al , 2009; Bourbousse et al , 2012; Hu et al , 2014; Zou et al , 2014; Zhou et al , 2017; Chen et al , 2019). HUB1 and HUB2 loss‐of‐function mutants ( hub1‐4 , hub2‐2 and the hub1‐4 hub2‐2 double mutant) exhibit salt‐sensitive phenotypes, and H2Bub1 regulates salt‐induced microtubule depolymerization and the PTP‐MPK3/6 signaling module in response to salt stress (Zhou et al , 2017) (MPK, Mitogen‐Activated Protein Kinase).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to flowering, H2Bub1 also regulates the expression levels of some circadian clock genes by increasing the H3K4me3 deposition in their chromatin (Himanen et al , 2012; Malapeira et al , 2012). Moreover, the overexpression of AtHUB2 enhances the level of H2Bub1 and H3K4me3 modifications at the GhDREB locus, leading the GhDREB gene to respond quickly to drought stress, which improves transgenic cotton's drought resistance (Chen et al , 2019). However, how H2Bub1 regulates transcription induction in plant responses to salt stress remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, H2Bub1 directly modulates the expression of the R gene SNC1 and impacts plant immune responses (Zou et al , 2014). H2Bub1 is associated with H3K4me3 at the GhDREB locus, and a greater level of H3K4me3 modification in chromatin regions of GhDREB triggers more rapid responses to drought stress in AtHUB2 transgenic cotton (Chen et al , 2019). Moreover, HUB1 and HUB2 play important regulatory roles in responses to salt stress (Zhou et al , 2017).…”

Section: Introductionmentioning

confidence: 99%

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E2 conjugases UBC1 and UBC2 regulate MYB42‐mediated SOS pathway in response to salt stress in Arabidopsis

Sun

Zhao

et al. 2020

New Phytologist

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Summary Histone H2B monoubiquitination (H2Bub1) is recognized as a crucial eukaryotic regulatory mechanism that controls a range of cellular processes during both development and adaptation to environmental changes. In Arabidopsis, the E2 conjugated enzymes UBIQUITIN CARRIER PROTEINs (UBCs) ‐1 and ‐2 mediate ubiquitination of H2B. Here, we elucidated the functions of UBC1 and ‐2 in salt‐stress responses and revealed their downstream target genes. Real‐time quantitative PCR assays showed that UBC1 and ‐2 positively regulated the salt‐induced expression of MYB42 and Mitogen‐Activated Protein Kinase 4 (MPK4). Chromatin immunoprecipitation assays revealed that H2Bub1 was enriched weakly on the chromatin of MYB42 and MPK4 in the ubc1,2 mutant. We further determined that UBC1/2‐mediated H2Bub1 enhanced the level of histone H3 tri‐methylated on K4 (H3K4me3) in the chromatin of MYB42 and MPK4 under salt‐stress conditions. MPK4 interacted with and phosphorylated MYB42. The MPK4‐mediated MYB42 phosphorylation enhanced the MYB42 protein stability and transcriptional activity under salt‐stress conditions. We further showed that MYB42 directly bound to the SALT OVERLY SENSITIVE 2 (SOS2) promoter and mediated the rapid induction of its expression after a salt treatment. Our results indicate that UBC1 and ‐2 positively regulate salt‐stress responses by modulating MYB42‐mediated SOS2 expression.

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“…The absorbance of the upper phase was determined at 520 nm. The proline content in the samples was calculated according to the standard curve and the formula described by Chen et al (2019).…”

Section: Measurement Of Malondialdehyde (Mda) Proline and Soluble Sumentioning

confidence: 99%

MaCDSP32 From Mulberry Enhances Resilience Post-drought by Regulating Antioxidant Activity and the Osmotic Content in Transgenic Tobacco

et al. 2020

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Desiccation tolerance is a complex phenomenon that depends on the regulated expression of numerous genes during dehydration and subsequent rehydration. Our previous study identified a chloroplast drought-induced stress protein (MaCDSP32) in mulberry, a thioredoxin (Trx) that is upregulated under drought conditions and is likely to confer drought tolerance to transgenic plants. Mulberry (Morus spp.) is an ecologically and economically important perennial woody plant that is widely used in forest management to combat desertification. However, its stress tolerance physiology is not well understood. In this study, the functions of MaCDSP32 gene were investigated. The expression of MaCDSP32 exhibited a circadian rhythm and was induced by mild and severe water deficits. Under abiotic stress, MaCDSP32-overexpressing plants exhibited increased stress sensitivity with lower water retention capacity and more severe lipid peroxidation than the wild-type (WT) plants. Furthermore, the activity of superoxide dismutase (SOD), the contents of proline and soluble sugars and the expression of stress-related transcription factors were lower in the MaCDSP32-overexpressing plants than in the WT plants. However, the MaCDSP32-overexpressing lines exhibited stronger recovery capability after rewatering post-drought. Moreover, the SOD enzyme activity, proline content, and soluble sugar content were higher in the transgenic plants after rewatering than in the WT plants. The production of the reactive oxygen species (ROS) H 2 O 2 and O 2 − was significantly lower in the transgenic plants than in the WT plants. In addition, under abiotic stress, the MaCDSP32-overexpressing lines exhibited improved seed germination and seedling growth, these effects were regulated by a positive redox reaction involving MaCDSP32 and one of its targets. In summary, this study indicated that MaCDSP32 from mulberry regulates plant drought tolerance and ROS homeostasis mainly by controlling SOD enzyme activity and proline and soluble sugar concentrations and that this control might trigger the stress response during seed germination and plant growth. Overall, MaCDSP32 exerts pleiotropic effects on the stress response and stress recovery in plants.

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An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton

Cited by 54 publications

References 49 publications

Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

E2 conjugases UBC1 and UBC2 regulate MYB42‐mediated SOS pathway in response to salt stress in Arabidopsis

MaCDSP32 From Mulberry Enhances Resilience Post-drought by Regulating Antioxidant Activity and the Osmotic Content in Transgenic Tobacco

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