Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

Ma, Siqi; Tang, Ning; Xu, Li; Xie, Yongjun; Xiang, Denghao; Fu, Jie; Shen, Jianqiang; Yang, Jun; Tu, Haifu; Li, Xianghua; Hu, Honghong; Xiong, Lizhong

doi:10.1016/j.molp.2018.12.005

Cited by 40 publications

(35 citation statements)

References 60 publications

(80 reference statements)

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“…GmUBC9 showed the highest percent identities with AtUBC1/2 ( Figure 2A; Supplementary Figure S6). AtUBC1/2 is an important enzyme that mediates histone H2B monoubiquitination, thereby broadly impacting plant growth and development, such as drought stress responses and flowering (Cao et al, 2008;Xu et al, 2009;Chen et al, 2019;Ma et al, 2019). Our study confirmed that GmUBC9 has the same function in soybeans, and they are all involved in H2B monoubiquitination.…”

Section: Discussionsupporting

confidence: 73%

“…UBC gene family members have been documented and characterized in a range of plants (Cao et al, 2008;Xu et al, 2009;Chen et al, 2019;Ma et al, 2019). Herein, we conducted a genome-wide assessment of UBC genes in soybeans, identifying 91 such putative UBC genes in total ( Supplementary Table S1).…”

Section: Discussionmentioning

confidence: 99%

“…The results showed that the interacting proteins of GmUBC9 were mostly UBC (E2) members. Next, we looked at the literature related to UBC (E2), we found a UBC interacted with a HUB2 protein that is reportedly an E3 ligase that mediates the monoubiquitination of H2B (H2Bub1) (Cao et al, 2008;Chen et al, 2019;Ma et al, 2019). We therefore evaluated potential physical interactions between GmUBC9 (Glyma.03G199900) and GmHUB2 (Glyma.02G267300)/AtHUB2 via yeast two hybrid assays (Y2H), and found that these proteins do indeed interact ( Figure 9A).…”

Section: Gmubc9 Interacted With Hub2 An E3 Ubiquitin Ligase In Soybmentioning

confidence: 99%

“…In light of the fact that GmUBC9 interacts with GmHUB2 and AtHUB2, which act as E3 ligases for H2Bub1 and is a key regulator of resistance to drought conditions, we next wanted to evaluate the role of H2Bub1 in general in the context of responses to drought (Cao et al, 2008;Chen et al, 2019;Ma et al, 2019). We initially determined the overall H2Bub1 levels in wild-type Arabidopsis Col-0 and soybean hairy root CK lines (empty vector) under severe drought conditions (~10% relative soil water content).…”

Section: Overexpression Of Gmubc9 In Arabidopsis and Soybean Enhancedmentioning

confidence: 99%

“…H2Bub1 can impact histone methylation including H3K4me3 (Chen et al, 2019;Fiorucci et al, 2019) as well as H3K36me3 (Cao et al, 2008;Zhao et al, 2019), which regulate flowering time through modulating the expression of the floral repressor FLOWERING LOCUS C (FLC). H2Bub1 is centrally involved in plant drought responses and in abscisic acid (ABA) signaling as exemplified through two E3-like proteins: OsHUB2 and AtHUB2, respectively (Cao et al, 2015;Ma et al, 2019;Chen et al, 2019). At present, efforts toward understanding monoubiquitination, especially histone monoubiquitination, have made considerable progress in some other plants, such as tomato (Zhang et al, 2015) and cotton (Chen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of GmUBC9 Gene Enhances Plant Drought Resistance and Affects Flowering Time via Histone H2B Monoubiquitination

et al. 2020

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Ubiquitylation is a form of post-translational modification of proteins that can alter localization, functionality, degradation, or transcriptional activity within a cell. E2 ubiquitin-conjugating enzyme (UBC) and E3 ubiquitin ligases are the primary determinants of substrate specificity in the context of ubiquitin conjugation. Multiubiquitination modifies target proteins for 26S proteasome degradation, while monoubiquitination controls protein activation and localization. At present, research on the monoubiquitination, especially histone monoubiquitination, has mostly focused on model plants with relatively few on crop species. In this study, we identified 91 UBC-like genes in soybean. The chromosomal localization, phylogenetic relationships, gene structures, and putative cis-acting elements were evaluated. Furthermore, the tissuespecific expression patterns of UBC Class I genes under drought stress were also investigated. Among Class I genes, GmUBC9 induction in response to drought stress was evident, and so this gene was selected for further analysis. GmUBC9 localized to the nucleus and endoplasmic reticulum. The overexpression of GmUBC9 in Arabidopsis led to enhanced tolerance for drought conditions across a range of stages of development, while overexpression in soybean hairy roots similarly led to improvements in tolerance for drought conditions, increased proline content, and reduced MDA content in soybean seedlings compared to wild type plants. HISTONE MONOUBIQUITINATION 2 (HUB2), an E3-like protein involved in histone H2B ubiquitylation (H2Bub1), was found to interact with GmUBC9 through Y2H analysis and BiFC assays in Arabidopsis and soybean. Under drought conditions, the level of H2Bub1 increased, and transcription of drought response genes was activated in GmUBC9 transgenic Arabidopsis and soybean. In addition, GmUBC9 transgenic Arabidopsis and soybean showed a late-flowering phenotype and

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Gmubc9 Interacted With Hub2 An E3 Ubiquitin Ligase In Soybmentioning

confidence: 99%

Section: Overexpression Of Gmubc9 In Arabidopsis and Soybean Enhancedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of GmUBC9 Gene Enhances Plant Drought Resistance and Affects Flowering Time via Histone H2B Monoubiquitination

et al. 2020

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Approaches and determinants to sustainably improve crop production

Gojon

Nussaume

Luu

et al. 2022

Food and Energy Security

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Plant scientists and farmers are facing major challenges in providing food and nutritional security for a growing population, while preserving natural resources and biodiversity. Moreover, this should be done while adapting agriculture to climate change and by reducing its carbon footprint. To address these challenges, there is an urgent need to breed crops that are more resilient to suboptimal environments. Huge progress has recently been made in understanding the physiological, genetic and molecular bases of plant nutrition and environmental responses, paving the way towards a more sustainable agriculture. In this review, we present an overview of these progresses and strategies that could be developed to increase plant nutrient use efficiency and tolerance to abiotic stresses. As illustrated by many examples, they already led to promising achievements and crop improvements. Here, we focus on nitrogen and phosphate uptake and use efficiency and on adaptation to drought, salinity and heat stress. These examples first show the necessity of deepening our physiological and molecular understanding of plant environmental responses. In particular, more attention should be paid to investigate stress combinations and stress recovery and acclimation that have been largely neglected to date. It will be necessary to extend these approaches from model plants to crops, to unravel the relevant molecular targets of biotechnological or genetic strategies directly in these species. Similarly, sustained efforts should be done for further exploring the genetic resources available in these species, as well as in wild species adapted to unfavourable environments. Finally, technological developments will be required to breed crops that are more resilient and efficient. This especially relates to the development of multiscale phenotyping under field conditions and a wide range of environments, and use of modelling and big data management to handle the huge amount of information provided by the new molecular, genetic and phenotyping techniques.

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Decrypting Drought Stress Tolerance of Crop Plants via Photosynthesis and Antioxidative Defense Mechanisms

Zulfiqar

Aslam

Rehman

et al. 2022

Antioxidant Defense in Plants

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Reversible Histone H2B Monoubiquitination Fine-Tunes Abscisic Acid Signaling and Drought Response in Rice

Cited by 40 publications

References 60 publications

Overexpression of GmUBC9 Gene Enhances Plant Drought Resistance and Affects Flowering Time via Histone H2B Monoubiquitination

Overexpression of GmUBC9 Gene Enhances Plant Drought Resistance and Affects Flowering Time via Histone H2B Monoubiquitination

Approaches and determinants to sustainably improve crop production

Decrypting Drought Stress Tolerance of Crop Plants via Photosynthesis and Antioxidative Defense Mechanisms

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