Abstract:O sucesso do estabelecimento de uma relação parasitária entre fungos e plantas, em muitos casos, depende de eventos que antecedem à infecção. Neste trabalho, as fases compreendidas entre a germinação e a penetração do fungo Stenocarpella macrospora foram analisadas por meio de microscópio eletrônico de varredura. Para tanto, plantas de milho (Zea mays) híbrido Das-8492, suscetível à mancha foliar de diplodia, foram cultivadas em casa de vegetação e inoculadas com 300 µl de uma suspensão de 10(5) conídios/ml ao… Show more
“…However, for leaves treated with India clove oil, appressorium formation occurred at 25h after inoculation. In the same way, BRUNELLI et al (2005) conducted SEM analysis and found that 86% of the conidia of Stenocarpella macrospora germinated between 12 and 15h after inoculation and appressorium formation occurred at 18h.a.i. Furthermore, oils from India clove and thyme caused wilting of some conidia at 30h.a.i.…”
Section: Discussionmentioning
confidence: 99%
“…The parasitic relationship between S. maydis and maize leaves may be well understood using the Scanning electron microscope (SEM) analysis, which is a valuable tool to study adhesion, germination, penetration, colonization, and reproduction of pathogens (ALVES et al, 2008). In this way, BRUNELLI et al (2005) and MEDICE el at. (2007) verifi ed that the essential oils act on the conidia germination and on the appessorium formation as well.…”
“…However, for leaves treated with India clove oil, appressorium formation occurred at 25h after inoculation. In the same way, BRUNELLI et al (2005) conducted SEM analysis and found that 86% of the conidia of Stenocarpella macrospora germinated between 12 and 15h after inoculation and appressorium formation occurred at 18h.a.i. Furthermore, oils from India clove and thyme caused wilting of some conidia at 30h.a.i.…”
Section: Discussionmentioning
confidence: 99%
“…The parasitic relationship between S. maydis and maize leaves may be well understood using the Scanning electron microscope (SEM) analysis, which is a valuable tool to study adhesion, germination, penetration, colonization, and reproduction of pathogens (ALVES et al, 2008). In this way, BRUNELLI et al (2005) and MEDICE el at. (2007) verifi ed that the essential oils act on the conidia germination and on the appessorium formation as well.…”
“…The field fungi require high humidity to grow, as the Stenocerpella complex, Diplodia complex scientific name, consisted of two species, Stenocarpella maydis (Berk.) Sutton, and Stenocarpella macrospora (Earle) Sutton (Brunelli et al, 2005). Currently, the two Stenocarpella species can be detected in all corn producing areas in the country, causing symptoms of diplodia stalk rot, white ear rot and macrospora stain on leaves (Casa et al, 2006).…”
Damage caused by Stenocarpella complex greatly reduce corn yield. This work was carried out to evaluate the relation between corn seeds physiological quality of a susceptible genotype and a tolerant genotype submitted to different S. maydis and S. macrospora inoculum potential, separately. Fungal colonies were grown on BDA plus mannitol culture medium, with water potential adjusted at-1.4 MPa. Seeds were inoculated for 0, 24, 48, and 72 hours. Evaluations were carried through seed health test, germination, germination first count, seedling emergence, speed of emergence index (SEI), cold test without media, and esterase enzyme expression. The highest incidence of Stenocarpella sp. was observed in the susceptible genotype. The tolerant genotype did not show typical structures of Stenocarpella complex at seven days. The increase of inoculum potential of S. maydis and S. macrospora on corn seeds generate physiological quality reduction. This quality reduction can be observed from 24 hours of inoculation for the susceptible genotype. The tolerant genotype was not physiologically affected, regardless the inoculation time to which they were submitted. The esterase analyses helped to differ S. maydis and S. macrospora inoculates and can also differ corn seed genotypes, however the reduction of seed quality observed with inoculation of the fungus cannot be detected only by viewing the bands in the gel.
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