Genotyping of Toxoplasma gondii isolates from free range chickens in the Pantanal area of Brazil

Soares, Rodrigo Martins; Silveira, LH; Silva, Aristeu Vieira da; Ragozo, Alessandra Mara Alves; Galli, Susiana; Lopes, Estela Gallucci; Gennari, Solange Maria; Pena, Hilda Fátima de Jesus

doi:10.1016/j.vetpar.2010.12.037

Cited by 35 publications

(27 citation statements)

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“…Analysis of the multilocus PCR-RFLP revealed 14 genotypes, suggesting high diversity of the parasite isolated from the human population in Minas Gerais, Brazil, similar to results reported by other authors in studies of parasites isolated from other host species (12,23,28). Of the 14 genotypes identified, four were identified in two or more isolates (genotypes 11, 41, 108, and 206), while the other 10 genotypes were found in only one isolate.…”

Section: Sag2 Sag3 Btub Gra6 C22-8 C29-2 L358 Pk1 Apicosupporting

confidence: 87%

“…Among the 14 genotypes, 7 were previously reported in animals and 7 were described for the first time in the literature (Table 2), with six found in only one isolate. This result reconfirmed the high diversity of T. gondii strains in Brazil (23,28,29).…”

Section: Sag2 Sag3 Btub Gra6 C22-8 C29-2 L358 Pk1 Apicosupporting

confidence: 75%

“…1). Isolate TgCTBr05 exhibited genotype 8 (also known as type BrIII), which was previously identified from domestic animals in other geographic regions of Brazil (12,23,25,26,28,29). Isolate TgCTBr18 exhibited genotype 36, which was previously identified from chickens in Rio de Janeiro, Brazil (23).…”

Section: Strain Isolation and Virulence Determination Of The T Gondimentioning

confidence: 88%

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Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

et al. 2013

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e Recent studies of Toxoplasma gondii isolates from animals in Brazil have revealed high genetic diversity. Many of these isolates are virulent to mice. It is speculated that these isolates may also be virulent to humans. However, there is very limited data regarding T. gondii strains from human infection. Therefore, it is not clear whether there is any association between parasite genotypes and disease phenotypes. In this study, a total of 27 T. gondii strains were isolated from humans with congenital toxoplasmosis in Minas Gerais state, Brazil. The genetic variability was assessed by restricted fragment length polymorphism in 11 loci (SAG1, 5= plus 3= SAG2, alternative [alt.] SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). Genetic analysis of 24 strains revealed 14 different genotypes, including 7 previously identified from animals and 7 new types. The widespread genotype BrII accounted for 29% (7/24) of the isolates and was the dominant genotype involved in this study. This is the first report of genotyping of T. gondii isolates obtained from blood samples from newborns with congenital toxoplasmosis. Genotypic characterization of these isolates suggests high genetic diversity of T. gondii in this human population in Brazil. Future studies are needed to determine the source of contamination of this human population.

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Section: Sag2 Sag3 Btub Gra6 C22-8 C29-2 L358 Pk1 Apicosupporting

confidence: 87%

Section: Sag2 Sag3 Btub Gra6 C22-8 C29-2 L358 Pk1 Apicosupporting

confidence: 75%

Section: Strain Isolation and Virulence Determination Of The T Gondimentioning

confidence: 88%

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Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

et al. 2013

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“…Espírito Santo of the strains prevalent in the environment, as FR chickens become infected mostly by feeding from soil contaminated with oocysts (Ruiz and Frenkel, 1980). In the last 12 years we and others have determined prevalence of T. gondii in FR chickens from different regions of Brazil (Peixoto and Lopes, 1990;Garcia et al, 2000;Dubey et al, 2002;da Silva et al, 2003;Dubey et al, 2003a,b;Brandão et al, 2006;Dubey et al, 2006Dubey et al, , 2007Aigner et al, 2010;de Oliveira et al, 2009;Soares et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Seroprevalence and genetic diversity of T. gondii isolates obtained from FR chickens from different regions of Brazil was summarized Dubey, 2010b;Soares et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espírito Santo state, southeastern Brazil

Beltrame

Pena

Ton

et al. 2012

Veterinary Parasitology

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Toxoplasma gondii: prevalence and characterization of new genotypes in free-range chickens from south Brazil

et al. 2018

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Toxoplasma gondii is an intracellular parasite that can infect all warm-blooded animals including humans. Recent studies showed that T. gondii strains from South America are genetically diverse. The present work aimed to determine T. gondii prevalence in free-ranging chicken in northwest Parana state in Brazil by two serological tests, to isolate the parasites from seropositive chickens and to genotype the isolates. Antibodies to T. gondii in 386 serum samples from 24 farms were investigated by immunofluorescence antibody assay (IFA) and modified agglutination test (MAT). Samples having titers ≥ 16 were considered positive for both tests. Among the 386 serum samples, 102 (26.4%) were positive for IFA, 64 (16.6%) were positive for MAT, 47 (12.2%) were positive in both tests, and 119 (30.8%) were positive in at least one of the two tests. Brain and pool of heart, lung, and liver from the 119 seropositive chickens were used for mouse bioassay to isolate the parasites. Thirty eight (31.9%) of these seropositive chickens were considered positives in mouse bioassay and 18 isolates were obtained. The isolates were characterized by 10 PCR-RFLP genetic markers including SAG1, SAG2 (5'-3'SAG2, alt.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Results of genotyping were compared with the genotypes in ToxoDB database. It revealed ten genotypes, including ToxoDB PCR-RFLP genotypes #6 (n = 2), #19 (n = 1), #21 (n = 2), #111 (n = 2), #152 (n = 1), and #175 (n = 1) and four new types not described before. Our results confirmed a high genetic diversity of this parasite in southern Brazil and also showed that the use of two serological tests in combination can improve the chance of T. gondii isolation. More studies should be taken to determine the zoonotic potential of chickens in the transmission of T. gondii.

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Genotyping of Toxoplasma gondii isolates from free range chickens in the Pantanal area of Brazil

Cited by 35 publications

References 24 publications

Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espírito Santo state, southeastern Brazil

Toxoplasma gondii: prevalence and characterization of new genotypes in free-range chickens from south Brazil

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