Genotyping of Staphylococcus aureus isolated from various sites on farms with dairy sheep using pulsed-field gel electrophoresis

Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese

“…Results demonstrate that all isolates presented antimicrobial activity against both Grampositive and Gram-negative bacteria, and against spoilage and pathogenic bacteria. Previously, antimicrobial activity against microorganisms involved in mastitis and other diseases of sheep flock was also reported (7,26,35). As shown in Table 1 On the other hand, antimicrobial activity against Enterococcus faecalis was not observed (Table 1).…”

Characterization of 26 Isolates of Staphylococcus Aureus, Predominantly from Dairy Sheep, Using Four Different Techniques of Molecular Epidemiology

“…In contrast to bovine mastitis, little information is available in the field of molecular epidemiology of S. aureus recovered from subclinical ewe mastitis. Although there is little doubt that the primary and most important reservoir of S. aureus is the infected mammary gland of the ewe, 19 more extensive studies are needed to increase the knowledge of the authors regarding other possible reservoirs, the transmission pathways, the dynamics of infection, the risk factors, and the clonal associations among isolates. Although S. aureus is considered to be an opportunistic pathogen, it is possible that certain clones are more prone to adhere to and colonize ewe udder because of the presence of particular virulence factors increasing their potential for adherence and colonization in comparison with other clones.…”

“…In this study, the authors compared the profiles of 26 strains using 4 different techniques: pulsed-field gel electrophoresis (PFGE), restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA-polymerase chain reaction (PCR) (RAPD), and multilocus sequence typing (MLST). Sixteen of the 26 strains of S. aureus were isolated from ovine subclinical mastitis in different flocks without any epidemiological link and in a limited time span (7 months) 19 ( Table 1).…”

“…19 chromosomal DNA from bacterial cultures was performed with the restriction enzyme SmaI before testing, using the CHEF-DR III Pulsed Field Electrophoresis Systems, a as described previously. 19 On each gel, 2 control plugs with the reference strain (strain CIP 57.10 b ) were included.…”

“…14 However, because PFGE still provides more information due to its high discriminatory power, it remains the technique of choice for typing bacteria in longitudinal studies. 11,19,20 Clearly, MLST and PFGE are the most powerful techniques for high-precision epidemiological typing when episodes of animal to animal transmission need to be fully investigated. Moreover, MLST is an adequate technique for generating genetic profiles of a large number of isolates, especially when comparing large international strain collections.…”