Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese

Abstract

Lactic acid bacteria (LAB) were isolated from ovine milk and cheeses manufactured in the South Region of Brazil. Among 112 bacterial isolates investigated, 59 were chosen through a screening for LAB. Among these 59 strains of LAB, 21% showed antimicrobial, proteolytic and lipolytic activities. Based on this screening, Lactobacillus plantarum LCN 17 and Lactobacillus rhamnosus LCN 43 were selected and tested for the production of bacteriocin-like substances (BLS). The BLS produced by both isolates showed antimicrobial activity against Listeria monocytogenes, whereas that produced by L. plantarum LCN 17 presented higher stability to different temperature, pH and enzyme treatments. These strains present potential for production of BLS, and for use as starter cultures.

In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry

“…LAB are weakly proteolytic compared with other groups of bacteria such as Bacillus, Proteus, Pseudomonas and Coliforms [61] but the bacterial strains do cause a significant degree of proteolysis in many fermented dairy products [62]. LAB is capable of hydrolyzing oligopeptides into small peptides and amino acids as it possess a very comprehensive proteinase/peptidase system [63]. Many dairy starter cultures are proteolytic thus bioactive peptides can be generated and used in the manufacturing of fermented dairy products.…”

Inhibition ofListeria monocytogenesbiofilms by bacteriocin-producing bacteria isolated from mushroom substrate

“…Therefore, the choice of medium is an important part of designing a strategy for isolation of antilisterial activity. Many studies have used MRS and M17 for the isolation of bacteriocin‐producing bacteria (Nespolo and Brandelli ; Yang et al . ; Woraprayote et al .…”

“…Dairy products, mainly cheese, are a major source of indigenous bacteriocin‐producing LAB such as Lactococcus and Enterococcus sp. (Nespolo and Brandelli ; Yang et al . ; Winkelströter et al .…”

“…Therefore, the choice of medium is an important part of designing a strategy for isolation of antilisterial activity. Many studies have used MRS and M17 for the isolation of bacteriocin-producing bacteria (Nespolo and Brandelli 2010;Yang et al 2012; where: mMRS is the control for ALB79 + mMRS; ALB79 + mMRS, reduction of biofilm by CFS of ALB79 in mMRS; TSBYE is control for ALB68 + TSBYE and ALB93 + TSBYE); ALB68 + TSBYE, reduction of L. monocytogenes isolates biofilm by CFS of ALB68 in TSBYE; ALB93 + TSBYE, reduction of biofilm by CFS of ALB93 in TSBYE; BHIYE, brain-heart infusion broth supplemented with 0Á6% yeast extract (BHIYE general control for biofilm formation by all L. monocytogenes isolates tested). See Fig.…”

Bioactive peptides in water-soluble extracts of ovine cheeses from Southern Brazil and Uruguay

“…The production of ewe's cheese is recent in Brazil and located in the southernmost regions -Santa Catarina and Rio Grande do Sul States, for climatic and geographic reasons (Nespolo & Brandelli, 2010). Two cheese manufacturers were from Rio Grande do Sul State (A-RS, and B-RS).…”