Characterization of 26 Isolates of <i>Staphylococcus Aureus</i>, Predominantly from Dairy Sheep, Using Four Different Techniques of Molecular Epidemiology

Vautor, Eric; Jay, Corinne; Chevalier, Nicolas; Visomblin, Nathalie; Vernet, Guy; Pépin, Michel

doi:10.1177/104063870501700411

Cited by 11 publications

(9 citation statements)

References 21 publications

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“…On the other hand, the most of the isolates originating from cows and sheep belonged to a limited number of coa subtypes (C3 accounting for 49% of bovine isolates and D2 accounting for 67% of ovine isolates). These data suggest that a limited number of closely related S. aureus strains, with a broad geographical distribution, may be responsible for most cases of IMI in many farms even in distant areas, as previously described in other countries (Fitzgerald et al, 1997;Stephan et al, 2001;Scherrer et al, 2004;Vautor et al, 2005). Interestingly, no predominant coa subtype was observed in isolates from goats.…”

Section: Discussionsupporting

confidence: 74%

Molecular Typing of Staphylococcus aureus Isolated from Cows, Goats and Sheep with Intramammary Infections on the Basis of Gene Polymorphisms and Toxins Genes

Vimercati¹,

Cremonesi²,

Castiglioni³

et al. 2006

Journal of Veterinary Medicine, Series B

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We investigated 116 Staphylococcus aureus isolates from cows, goats and sheep with intramammary infections (IMI) in Italy to provide information about the spread of enterotoxigenic strains and to compare strains isolated from different ruminant species. The isolates were typed by restriction fragment length polymorphism (RFLP) analysis of the coagulase (coa) gene, by analysis of polymorphisms of the X region of protein A (spa) gene and by detection of genes encoding enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, sej and sel). Seven different coa types and 12 different spa types were distinguished. On the basis of polymerase chain reaction-RFLP, 29 different coa subtypes were identified. Two different coa subtypes accounted for 49% and 67% of bovine and ovine isolates respectively. Only seven coa subtypes were observed in isolates from more than one host species and no coa subtype was present in isolates from all three ruminant species. Furthermore, 85 of the isolates (73%) harboured at least one enterotoxin gene (se) with a predominance of sea, sed and sej among isolates from bovine IMI, and sec and sel among isolates from caprine and ovine IMI. Comparing the S. aureus isolates on the basis of gene polymorphisms and presence of se genes, significant differences were found in distributions of genotypes among isolates from cows, goats and sheep.

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Section: Discussionsupporting

confidence: 74%

Molecular Typing of Staphylococcus aureus Isolated from Cows, Goats and Sheep with Intramammary Infections on the Basis of Gene Polymorphisms and Toxins Genes

Vimercati¹,

Cremonesi²,

Castiglioni³

et al. 2006

Journal of Veterinary Medicine, Series B

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“…All 122 isolates considered in this study were characterized by means of RAPD-PCR, a technique used by many to type S. aureus isolated from different foodstuffs implicated in staphylococcal food poisoning [32–35], from individual quarter milk and human samples [36–40] and from mastitis milk samples [41]. The RAPD-PCR analyses on all the isolates were carried out with the primers M13 and AP4.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and Genotypic Characterization ofStaphylococcus aureusStrains from Italian Dairy Products

Morandi

Brasca

Andrighetto³

et al. 2009

International Journal of Microbiology

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Staphylococcus aureus is a known major cause of foodborne illnesses, and milk and dairy products are often contaminated by enterotoxigenic strains of this bacterium. In the present study, 122 S. aureus isolates collected from different dairy products were characterised by phenotypic properties, by the distribution of genes encoding staphylococcal enterotoxins (sea, sec, sed, seg, seh, sei, sej, and sel) and by randomly amplified polymorphic DNA PCR (RAPD-PCR). Moreover, strain resistance to vancomycin and methicillin (oxacillin) was studied. The differences in the RAPD-PCR profiles obtained with the primers M13 and AP4 revealed the presence of a great genetic heterogeneity among the different S. aureus strains. Using the primer AP4 and M13, eight groups were distinguished by RAPD-PCR cluster analysis, although, except in few cases, it was not possible to correlate the isolates of different animal species (cow or ovine) with the presence of se genes. None of the isolates showed resistance to vancomycin or methicillin.

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“…For example, CC97 has been associated with bovine mastitis (Aires- de-Sousa et al, 2007;Monecke et al, 2007;Rabello et al, 2007;Smith et al, 2005) and ST398 is associated with pigs and horses (van Leeuwen et al, 2005). Although several studies have analysed the genetic diversity of bovine strains, only a small number of S. aureus strains from other animals such as sheep (Mork et al, 2005;Vautor et al, 2005Vautor et al, , 2009Ben Zakour et al, 2008;Sung et al, 2008), goats (Jorgensen et al, 2005;Ben Zakour et al, 2008;Sung et al, 2008;Vautor et al, 2009), horses (Sung et al, 2008), chickens (Rodgers et al, 1999) and rabbits (Vancraeynest et al, 2006) have been characterized. More recently, a study of S. aureus strains from bovine mastitis has shown the association of particular STs with virulence and severity of disease (Guinane et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular genetic typing reveals further insights into the diversity of animal-associated Staphylococcus aureus

Smyth

Feil

Meaney

et al. 2009

Journal of Medical Microbiology

104

102

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Staphylococcus aureus is an important pathogen of man, but is also able to colonize and cause disease in a wide variety of mammals and birds. An extended multilocus sequencing approach, involving multilocus sequence typing (MLST), sas typing, spa typing and agr typing, was used to examine the molecular diversity of 118 S. aureus isolates recovered from a range of host species and to compare these data with the known diversity of human-derived isolates. MLST revealed that the commonest animal-associated MLST types were ST133, ST5, ST71, ST97, ST126 and ST151. ST133 appears to be an ungulate-animal-specific genotype, as no evidence of ST133 associating with humans has yet been found in the literature. Novel and unique sas alleles were identified in the animal-associated strains that may represent animal-associated sas alleles. However, sas typing exhibited a lower typeability than MLST for the animal strains (91.3 %). Phylogenetic analyses using neighbour-joining and maximum-parsimony trees localized ruminantassociated MLST lineages to both previously identified S. aureus subspecies aureus subgroups, thus explaining the finding of all four agr types within the ruminant-associated strains. S. aureus isolates recovered from chickens and rabbits were genotypically more similar to known human genotypes than the ruminant-associated lineages.

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Characterization of 26 Isolates of Staphylococcus Aureus, Predominantly from Dairy Sheep, Using Four Different Techniques of Molecular Epidemiology

Cited by 11 publications

References 21 publications

Molecular Typing of Staphylococcus aureus Isolated from Cows, Goats and Sheep with Intramammary Infections on the Basis of Gene Polymorphisms and Toxins Genes

Molecular Typing of Staphylococcus aureus Isolated from Cows, Goats and Sheep with Intramammary Infections on the Basis of Gene Polymorphisms and Toxins Genes

Phenotypic and Genotypic Characterization ofStaphylococcus aureusStrains from Italian Dairy Products

Molecular genetic typing reveals further insights into the diversity of animal-associated Staphylococcus aureus

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