Genotyping and distribution of virulence factors in V. parahaemolyticus from seafood and environmental sources, South-west coast of India

Silvester, Reshma; Madhavan, A; Antony, Ally C.; Alexander, Deborah; Sudha, S.; Francis, Bini; Hatha, Mohamed

doi:10.1016/j.rsma.2017.03.007

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…In a previous study on V. parahaemolyticus isolated from seafood from Cochin, Kerala using ERIC-PCR, grouping of isolates based on the source of isolation was reported (Chakraborty et al, 2013). Another study on genotyping of V. parahaemolyticus from the same geographical area reported that the isolates from different location and source fell into the same cluster (Silvester et al, 2017). Though our observations coincide with both these studies, the influence of geographical factor could be dismissed as all the isolates from single geographical location did not fall into one cluster.…”

Section: Discussionsupporting

confidence: 82%

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

et al. 2020

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Narayanan et al. V. parahaemolyticus With Pathogenic Potential in Seafood The conventional methods used to identify the pathogenic V. parahaemolyticus would be good for clinical isolates, but a more elaborate method is recommended for the detection of tdh gene in environmental isolates. This is the first comprehensive study on pathogenic V. parahaemolyticus in Kerala, India and demonstrates for the first time, the isolation of potentially pathogenic V. parahaemolyticus, carrying tdh gene from seafood collected from retail markets in Kerala.

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Section: Discussionsupporting

confidence: 82%

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

et al. 2020

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“…This shows the capability of V. parahaemolyticus strains to cause beta-hemolysis of human erythrocytes, thereby posing a risk to consumers of these seafoods. A previous study among V. parahaemolyticus from Cochin estuary, around 42.3% of the isolates produced hemolysin and 23.5% produced urease (Silvester et al, 2017). Lipases and phospholipases were also produced by our strains; these enzymes mainly contribute in nutrient acquisition and degrading the membrane lipid of the host, thus involving in the pathogens's virulence (Costa et al, 2013).…”

Section: Discussionsupporting

confidence: 48%

“…Plate assay was used for all the enzymes except for urease which was screened by tube test. Enzyme screening methods previously described in Silvester et al (2017) was followed.…”

Section: Phenotypic Virulence Screeningmentioning

confidence: 99%

Increased incidence and antimicrobial resistance among Vibrio parahaemolyticus in shellfishes from major fish markets in Cochin, South India: Seafood risk assessment

Silvester

Saji²,

Divakaran³

et al. 2022

Annals of Animal Science

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India remains as a top supplier of value-added seafoods to the global markets. Cochin is one of the leading fishing ports in India contributing to country’s major seafood export. As part of risk assessment, we analyzed the prevalence of multi-drug resistant Vibrio parahaemolyticus; seafood borne gastroenteritis-causing pathogen, in shellfishes collected from major fish markets in Cochin. This bacterial species was initially isolated on Thiosulfate-citrate-bile salts-sucrose agar and HiCrome™ Vibrio agar, further confirmed by detection of V. parahaemolyticus species-specific toxR gene and by 16S r RNA sequencing. A total of 113 confirmed V. parahaemolyticus were recovered. Almost all the strains exhibited resistance towards three or more antibiotics (multiple-drug resistant) and harbored virulence related exoenzymes especially hemolysin (Kanagawa phenomenon); which is indeed a matter of concern. The multiple antibiotic resistance (MAR) index of the isolates ranged from 0.21 to 0.57. Furthermore, our results also indicate a substantial increase in the incidence of the pathogen in seafood from the area compared to previous years. This may pose a risk to the local and international consumers of the seafood. Practicing complete hygiene and adequate cooking is strongly recommended. Regular monitoring of aquaculture areas and fish markets is urged to reduce dissemination of pathogenic V. parahaemolyticus in seafood and to prevent fatal cases of human infection induced by this species in the country.

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Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance

Narayanan

Joseph

Peeralil

et al. 2020

Marine Pollution Bulletin

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Genotyping and distribution of virulence factors in V. parahaemolyticus from seafood and environmental sources, South-west coast of India

Cited by 5 publications

References 43 publications

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Increased incidence and antimicrobial resistance among Vibrio parahaemolyticus in shellfishes from major fish markets in Cochin, South India: Seafood risk assessment

Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance

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