Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Narayanan, Sreejith Viyyokkaran; Joseph, Toms C.; Peeralil, Shaheer; Mothadaka, Mukteswar Prasad; Lalitha, K.V.

doi:10.3389/fmicb.2020.00592

Cited by 31 publications

(39 citation statements)

References 57 publications

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“…Also, PCR-detected serotyping of 145 isolates for O-antigen by Xu et al (2016) revealed that serotypes with the exemption of O6, O9, and O7, were detected which was not in line with our study as O6 and O7 serotypes were detected in our study. Furthermore, our findings disagree with the study of Zhao et al (2011) that detected the O3 serotype as the most detected serotype from shellfish and the study of Narayanan et al (2020) that reported the prevalence O1 serotype in V. parahaemolyticus recovered from retail food and water samples. Location and serogroups did not correlate the phenotypic and/or genotypic profile of the V. parahaemolyticus in our study.…”

Section: Discussioncontrasting

confidence: 99%

“…The level of V. parahaemolyticus prevalence in this study appears to be lower when compared to the findings of Lee et al (2018) in which 69% (165/240) of the analyzed samples were known to be positive to V. parahaemolyticus (based on toxR PCR assay). Similarly, the study of Tan et al (2017) and Narayanan et al (2020) showed the prevalence of V. parahaemolyticus in samples analyzed as 79.5 and 90% respectively. Contrarily, the study of Yang et al (2017) demonstrated a lower level V. parahaemolyticus prevalence as 19.44% in tested samples.…”

Section: Discussionmentioning

confidence: 82%

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Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria

et al. 2021

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The demand for minimally processed vegetables (African salad) has increased partly due to its inclusion in ready-to-eat foods. Nevertheless, the associated risk of the presence of emergent foodborne pathogens, such as Vibrio parahaemolyticus might be underestimated. The present study was designed to isolate and characterize foodborne V. parahaemolyticus from minimally processed vegetables using culture-based methods and molecular approach. A total of 300 samples were examined from retail outlets between November 2018 and August 2019 from Southern Nigeria. The prevalence of vibrios from the overall samples based on the colonial proliferation of yellow, blue-green and/or green colonies on thiosulfate citrate bile salts sucrose agar was 74/300 (24.6%). An average of two green or blue-green colonies from respective plates was screened for V. parahaemolyticus using analytical profile index (API) 20 NE. Polymerase chain reaction further confirmed the identity of positive V. parahaemolyticus. The counts of V. parahaemolyticus ranged from 1.5 to 1,000 MPN/g. A total of 63 recovered V. parahaemolyticus were characterized further. The resistance profile of the isolates include ampicillin 57/63 (90.5%), cefotaxime 41/63 (65.1%), ceftazidime 30/63 (47.6%), amikacin 32/63 (50.8%), kanamycin 15/63 (23.8%), and oxytetracycline 16/63 (25.4%). The multiple antibiotic index ranged from 0–0.81. The formation of biofilm by the isolates revealed the following: strong formation 15/63 (23.8%), moderate formation 31/63 (49.2%), weak formation 12/63 (19.1%), and no formation 5/63 (7.9%). A total of 63/63 (100%), 9/63 (14.3%), and 20/63 (31.8%) of the isolates harbored the tox R gene, TDH-related hemolysin (trh) and thermostable direct hemolysin (tdh) determinants respectively. The isolates with O2 serogroup were most prevalent via PCR. Isolates that were resistant to tetracycline, kanamycin, and chloramphenicol possessed resistant genes. The presence of multidrug-resistant vibrios in the minimally processed vegetables constitutes a public health risk and thus necessitates continued surveillance.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 82%

Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria

et al. 2021

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“…The isolates were subjected to the antibiotic susceptibility test by the Kirby disc diffusion method [89] . This method has been employed in several studies investigating the antibiotic sensitivity in V. parahaemolyticus isolated from food samples [90,91] . All the isolates were revived in Tryptic Soy Broth (TSB) (HiMedia, India) supplemented with NaCl (2% w/v) and shaken at 37°C for 18 hours at 200 RPM.…”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Venggadasamy

Loh²,

Law

et al. 2021

Prog Micobes Mol Biol

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Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently.

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“…Recently, in exception to the vibrios directly isolated from shrimp aquaculture system, reports of antibiotic‐resistant Vibrio sp. were identified from the commercial fish market samples (Kumar & Lalitha, 2013; Letchumanan et al, 2015; Madhusudana Rao & Surendran, 2010; Narayanan, Joseph, Peeralil, Koombankallil, et al, 2020; Narayanan, Joseph, Peeralil, Mothadaka, et al, 2020; Obaidat et al, 2017; Sperling et al, 2015; Wong et al, 2012; Yu et al, 2016).…”

Section: Amu Amr and Arg's In Vibriosmentioning

confidence: 99%

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

Vaiyapuri

Pailla

Badireddy

et al. 2021

Aquaculture Research

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Antimicrobial resistance (AMR) is a growing threat to the human, and animal population, worldwide. The antimicrobials used across all the sectors eventually pollute the aquatic environment and transform it into a hot spot for the emergence and spread of AMR. The bacteria under the genera Vibrio being ubiquitous to aquatic environment include a few species which are pathogenic to humans and aquatic animals. Despite extensive reviews available on AMR in human, terrestrial animal sectors and environment, there is a dearth of summarized information on AMR in Vibrios in shrimp aquaculture environment. In comparison to the human, and terrestrial animal health sector, a methodology for determination of AMR in vibrios associated with aquatic animals is still in its nascent stages and needs harmonization. The present review is centred on the AMR status in vibrios of shrimp farming environment and recent advances in the detection of resistance and discusses methodologies for AMR determination in vibrios and possible mitigation measures to control AMR spread.

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Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Cited by 31 publications

References 57 publications

Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria

Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

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