Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance

Narayanan, Sreejith Viyyokkaran; Joseph, Toms C.; Peeralil, Shaheer; Koombankallil, Reshmi; Vaiyapuri, Murugadas; Mothadaka, Mukteswar Prasad; Lalitha, K.V.

doi:10.1016/j.marpolbul.2020.111551

Cited by 27 publications

(17 citation statements)

References 55 publications

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“…imipenem (IPM), chloramphenicol (C), doxycycline (DO) and cotrimoxazole (COT) irrespective of the species. In consonance with our findings, high susceptibility of vibrios against these antibiotics has been reported by different authors (Ottaviani et al, 2001;Al-Othrubi et al, 2014;Narayanan et al, 2020). There was no 100% resistance to any of the recommended antibiotics at the genus level.…”

Section: Discussionsupporting

confidence: 93%

“…It has been now recommended that assessment of AMR and identification of association between different AMR characteristics, can prevent the further selection of resistance, configuring critical tool in devising efficient control guidelines (Deng et al, 2020). Despite the alarming rise in reports on prevalence of vibrios from sea food samples and, vibriosis outbreaks from both aquaculture practices and from human health sector, little information is available on the virulence and AMR characteristics of vibrios in diseased fishes of India (Parthasarathy et al, 2016;Narayanan et al, 2020). Further, certain ARGs (AMR encoding genes) and virulence genes in vibrios are proven to be horizontally transferred to other human pathogens (Neela et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance and virulence characteristics of Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi from natural disease outbreaks of marine/estuarine fishes

et al. 2021

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Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi are the causative agents of the most severe diseases of marine and brackish aquaculture systems. These are also associated with serious ailments in humans. The present paper unravels the virulence features/genes and antimicrobial resistance (AMR) phenotypes/genes of these Vibrio spp. isolated from natural disease outbreaks of marine/estuarine fishes of India for the first time. Results on virulence features showed that V. vulnificus infected fish can pose public health risk. While, it has been found clinically that V. parahaemolyticus without tdh and trh genes are pathogenic to fish, even though they are reported to be, not associated with human diseases. It was significant to note that virulence features of V. harveyi were induced at increased salinity. Analysis based on the percentage prevalence of susceptible isolates and variation coefficient of zone diameters categorized 17 antibiotics in terms of their efficiency against each fish pathogenic species. Multiple antibiotic resistance index (MARi) of the isolates ranged between 0.058 and 0.47. Results on MARi and percentage of multidrug resistance strains indicated that >50% of the isolates were from low antibiotic usage area. The study generated tetH sequence from V. parahaemolyticus for the first time, and the sequence revealed high identity to that of clinical strains. Presence of tetB/ tetH gene was identified as the predictor for the resistance against the first generation tetracycline, the most commonly used antibiotic against Vibrio spp. in aquaculture practices. The data on associations between AMR features predicted certain cross-resistance between antimicrobials within Vibrio spp. Altogether, the paper serves as the baseline for epizootic tracking of public health significant vibrios from diseased fishes, to devise practical guidelines for antibiotic use and to formulate efficient control measures against three Vibrio spp. in aquaculture, targeting final applications in the implementation of national green and healthy aquaculture practices.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and virulence characteristics of Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi from natural disease outbreaks of marine/estuarine fishes

et al. 2021

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“…Among the bacterial diseases, vibriosis caused by various vibrios is causing tremendous economic losses and is responsible for massive mortality of cultured shrimp (Novriadi, 2016). The Harveyi clade of vibrios, including closely related species such as Vibrio campbellii , Vibrio harveyi and Vibrio parahaemolyticus , includes major pathogens of aquaculture organisms (Austin & Zhang, 2006; Dong et al, 2016; Narayanan et al, 2020). Vibrio harveyi is a serious pathogen of both marine vertebrates and invertebrates including fish and crustaceans, particularly penaeid shrimp (Austin & Zhang, 2006).…”

Section: Introductionmentioning

confidence: 99%

Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade

Zhang

Yang

Defoirdt

2021

J of Applied Microbiology

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Aim: Indole is a signaling molecule secreted by over 85 species of bacteria, including several Vibrio species, and it has been reported to affect different bacterial phenotypes such as biofilm formation, motility, and virulence. In this study, we aimed at investigating the inter-strain variability of the effect of indole in 12 different strains belonging to the Harveyi clade of vibrios. Methods and Results:Indole reduced the virulence of all strains towards gnotobiotic brine shrimp larvae. The survival rate of brine shrimp larvae challenged with vibrios pretreated with indole was increased by 1.3-fold to 1.8-fold. Additionally, indole significantly decreased the biofilm formation in all of the strains, decreased the swimming motility in eight of the strains, and decreased swarming motility in five of the strains. When cultured in the presence of exogenous indole, the mRNA level of the pirA and pirB toxin genes were down-regulated to 65% and 46%, and to 62% and 55% in the AHPND-causing strains Vibrio parahaemolyticus M0904 and Vibrio campbellii S01, respectively. Conclusions: These data indicate that indole has a significant impact on the virulence of different strains belonging to the Harveyi clade of vibrios. Significance and Impact of the Study: Our results suggest that indole signaling is a valid target for the development of novel therapeutics in order to control infections caused by Harveyi clade vibrios in aquaculture. K E Y W O R D S acute hepatopancreatic necrosis disease (AHPND), biofilm, early mortality syndrome (EMS), motility, quorum sensing How to cite this article: Zhang, S., Yang, Q. & Defoirdt, T. (2022) Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade.

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“…Recently, in exception to the vibrios directly isolated from shrimp aquaculture system, reports of antibiotic‐resistant Vibrio sp. were identified from the commercial fish market samples (Kumar & Lalitha, 2013; Letchumanan et al, 2015; Madhusudana Rao & Surendran, 2010; Narayanan, Joseph, Peeralil, Koombankallil, et al, 2020; Narayanan, Joseph, Peeralil, Mothadaka, et al, 2020; Obaidat et al, 2017; Sperling et al, 2015; Wong et al, 2012; Yu et al, 2016).…”

Section: Amu Amr and Arg's In Vibriosmentioning

confidence: 99%

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

Vaiyapuri

Pailla

Badireddy

et al. 2021

Aquaculture Research

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Antimicrobial resistance (AMR) is a growing threat to the human, and animal population, worldwide. The antimicrobials used across all the sectors eventually pollute the aquatic environment and transform it into a hot spot for the emergence and spread of AMR. The bacteria under the genera Vibrio being ubiquitous to aquatic environment include a few species which are pathogenic to humans and aquatic animals. Despite extensive reviews available on AMR in human, terrestrial animal sectors and environment, there is a dearth of summarized information on AMR in Vibrios in shrimp aquaculture environment. In comparison to the human, and terrestrial animal health sector, a methodology for determination of AMR in vibrios associated with aquatic animals is still in its nascent stages and needs harmonization. The present review is centred on the AMR status in vibrios of shrimp farming environment and recent advances in the detection of resistance and discusses methodologies for AMR determination in vibrios and possible mitigation measures to control AMR spread.

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Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance

Cited by 27 publications

References 55 publications

Antimicrobial resistance and virulence characteristics of Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi from natural disease outbreaks of marine/estuarine fishes

Antimicrobial resistance and virulence characteristics of Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi from natural disease outbreaks of marine/estuarine fishes

Indole decreases the virulence of pathogenic vibrios belonging to the Harveyi clade

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

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