Genotypic variation in Acmella paniculata across different phytogeographical ranges of Northeast India inferred through ISSR &amp; SCoT based markers

Gupta, Debmalya Das; Hui, Pallabi K.; Tag, Hui

doi:10.1016/j.jarmap.2018.07.001

Cited by 3 publications

(3 citation statements)

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“…Among these molecular markers, Inter Simple Sequence Repeats (ISSRs) and Start Codon Targeted polymorphism (SCoT) are used efficiently for genetic diversity assessment of plants (Etminan et al, 2016). Many studies found that SCoT is might be more effective than other dominant DNA molecular markers like RAPD and ISSR because it is gene-targeted (Gupta et al, 2018). Also, SCoT is superior over these markers in higher polymorphism and better marker resolvability (Gorji et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Description and Evaluation of Some Newly Introduced Grape Cultivars Under Egyptian Conditions وصف وتقييم بعض أصناف العنب المستوردة حديثاً تحت الظروف المصرية

Ahmed

El-Aziz

2021

Journal of Agricultural Chemistry and Biotechnology

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This research was conducted for two consecutive seasons (2017 and 2018) in a private vineyard located at El-Sadat city, Menofia governorate to describe and evaluate three grape seedless cultivars namely: Early Sweet, Prime and Star light grape cultivars under Egyptian conditions. The results revealed that Early Sweet grape cultivar was the earliest cultivars concerning the phenological dates include bud burst, full bloom, fruit set, veraison and physiological ripening followed by Prime grape cultivar, whereas Star light grape cultivar was the latest in this respect through the two seasons of the study. All the studied cultivars succeeded under Egyptian conditions and characterized by high quality of bunch and berries. Genetic diversity among the three cultivars was assessed using SCoT and ISSR molecular marker techniques. Cluster analysis showed molecular and phenotypic variation among cultivars, which divided into three groups, each group was contained one cultivar. These cultivars were distinguished by 36 molecular markers 27 out of them were from SCoT markers which proved successful to target generic regions across the grape genome. These markers after deciphering their structure may help to evolve reliable molecular markers to select desirable traits in these cultivars. So, we recommend expansion in the cultivation of these cultivars under Egyptian conditions and benefit of our molecular results to elicit reliable molecular markers characterizing desirable traits in these cultivars to use in Egyptian grape cultivars improving programs.

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Section: Introductionmentioning

confidence: 99%

Description and Evaluation of Some Newly Introduced Grape Cultivars Under Egyptian Conditions وصف وتقييم بعض أصناف العنب المستوردة حديثاً تحت الظروف المصرية

Ahmed

El-Aziz

2021

Journal of Agricultural Chemistry and Biotechnology

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“…Moreover, according to Mao et al (2018), study of genetic diversity with a combined markers ISSR and SCoT found that the use of both markers give a very effective, reliable and more superior results than the use of single markers. Also, other studies have found that SCoT is very correlated with neutral markers like RAPD and ISSR, and as it is gene-targeted, it might be more effective than the other markers (Gupta et al, 2018). Biniari and Stavrakaki (2019) performed ampelographic and RAPD analysis to identify and discriminate between forty-nine Greece grapevine varieties.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Abdelaziz

El-Sherif

2020

Bangladesh J. Plant Taxon

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Grapevine is one of the major fruit trees in the Kingdom of Saudi Arabia. This study aims to discriminate and assess the genetic diversity in three grapevine cultivars in Al- Madinah through the combination of characterization using both classical ampelographic as well as ampelometric studies with molecular markers using Randomly Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Start Codon Targeted Polymorphism (SCoT). For the ampelographic analysis, twelve OIV descriptors were used, and for the ampelometric analysis, the fully expanded mature leaves area were automatedly measured. The genetic distance among the three grapevines cultivars, calculated using Jaccard's coefficient, ranged from 0.7577 (between AL Nakheel and Banati) to 0.4501 (between AL Nakheel and Ahmer). The molecular analysis was based on the use of thirty-one primers; ten RAPD primers, seven ISSR primers and fourteen SCoT primers. RAPD primers generated the highest polymorphism (67%), while the level of polymorphism with ISSR primers was 36% and with SCoT 44%. All of the three markers generated similar dendrograms, and the genetic distance generated with RAPD was higher compared with SCoT and ISSR. The three markers RAPD, ISSR and SCoT were combined and amalgamated with the morphological data, and combined dendrogram was generated and discussed. AL Nakheel and Ahmer cultivars were found to be more closely related to each other than Banati which was separated in a different cluster. Both methods were effectively efficient for complete identification of grapevine and for studying the genetic diversity between closely related cultivars. Bangladesh J. Plant Taxon. 27(1): 113-127, 2020 (June)

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“…Many studies found that SCoT might be more effective than other dominant DNA molecular markers like RAPD and ISSR because it is gene-targeted (Gupta et al, 2018). Also, SCoT is superior to these markers in higher polymorphism and better marker resolvability (Gorji et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques

Albrifcany¹,

Askander

2022

Journal of Agricultural Chemistry and Biotechnology

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This study was executed to evaluate molecular variations among 13 genotypes of Vicia faba from Egypt and Kurdistan of Iraq for use in crop breeding programs in Kurdistan. The genetic relationships have been detected among these genotypes using ISSR and SCoT markers. Ten and twelve primers had successfully generated reproducible polymorphic amplicons that were suitable for studying the genetic variability between studied genotypes. Out of 225 clear amplicons detected, (77 for ISSR and 92 for SCoT were polymorphic) with 70.71 and 70.03 polymorphism %, respectively. SCoT markers provided more discriminating data and were more informative with high UM%, DI, and Rp values (37.0, 0.23, and 3.81, respectively) compared with ISSR markers (25.7, 0.21, and 3.50, respectively). Cluster analysis and PCo analysis based on ISSR-SCoT combined data succeeded in the description of genetic diversity and heterogeneity within studied genotypes. Hence, setting the studied genotypes into four major groups. The first group included check variety only while the second group included sakha1, sakha3 and sakha4, the third group included only Giza843, and the last group contained all other genotypes, including all studied cultivars from Kurdistan. Crosses between divergent genotypes out of these groups may lead to the development of effective breeding strategies in the future. Therefore, we recommend more studies on the genetic improvement of these cultivars and their inclusion in breeding programs in Kurdistan of Iraq due to the significant genetic differences between them.

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Genotypic variation in Acmella paniculata across different phytogeographical ranges of Northeast India inferred through ISSR & SCoT based markers

Cited by 3 publications

References 34 publications

Description and Evaluation of Some Newly Introduced Grape Cultivars Under Egyptian Conditions وصف وتقييم بعض أصناف العنب المستوردة حديثاً تحت الظروف المصرية

Description and Evaluation of Some Newly Introduced Grape Cultivars Under Egyptian Conditions وصف وتقييم بعض أصناف العنب المستوردة حديثاً تحت الظروف المصرية

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques

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