Genotypic characterization and evaluation of an antibiotic resistance of Trueperella pyogenes (Arcanobacterium pyogenes) isolated from milk of dairy cows with clinical mastitis

Zastempowska, Ewa; Lassa, H.

doi:10.1016/j.vetmic.2012.07.018

Cited by 58 publications

(62 citation statements)

References 21 publications

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“…Collagen-binding protein (CbpA) is a virulence factor necessary for adhesion to host cells (Esmay et al, 2003;Pietrocola et al, 2007). In the present study, the cbpA gene encoding this protein was detected in all isolates (100%), while it was reported to have been recovered from 21% of bovine mastitis isolates (Zastempowska and Lassa, 2012). In addition, two neuraminidases, H (nanH: 40%) and P (nanP: 38%) were found in this study.…”

Section: Discussionsupporting

confidence: 47%

“…Both the nanH and nanP genes also occurred in all isolates recovered from the uterus of dairy cows (Silva and Lobato, 1999). In contrast, other studies reported that the nanH gene was present only in 20.2% (Zastempowska and Lassa, 2012) and 87% (Hijazin et al, 2011) of isolates of bovine mastitis samples. Furthermore, 46 T. pyogenes isolates (92%) carried the fimA gene and most of them (82%) simultaneously harboured the fimC gene, while fimG was found in 38% of the isolates.…”

Section: Discussionmentioning

confidence: 69%

“…These results are similar to the findings of a previous study (Silva and Lobato, 1999). The fimC gene seemed to occur more frequently in isolates from mastitis (88%) (Zastempowska and Lassa, 2012) than in those from metritis (67%) (Silva and Lobato, 1999). The fimG gene, in turn, was more prevalent among isolates from bovine metritis (50% or 67%) (Silva and Lobato, 1999;Santos et al, 2010) compared to those from bovine mastitis (18%) (Zastempowska and Lassa, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The fimC gene seemed to occur more frequently in isolates from mastitis (88%) (Zastempowska and Lassa, 2012) than in those from metritis (67%) (Silva and Lobato, 1999). The fimG gene, in turn, was more prevalent among isolates from bovine metritis (50% or 67%) (Silva and Lobato, 1999;Santos et al, 2010) compared to those from bovine mastitis (18%) (Zastempowska and Lassa, 2012). Previous research has found that the expression of fimbriae plays an important role in the pathogenicity and biofilm formation of Salmonella Enteritidis and E. coli (Pruss et al, 2006;Soto et al, 2007;Lasaro et al, 2009;Zhu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, most aspects of the pathogenesis of infection caused by T. pyogenes originating exclusively from bovine mastitis have been poorly characterised to date (Zastempowska and Lassa, 2012). The aim of this study was to explore the pathogenesis and antibiotic resistance of T. pyogenes strains isolated from bovine mastitis cases in China.…”

mentioning

confidence: 99%

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Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Alkasir

Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella (T.)pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.Key words: Trueperella pyogenes, bovine mastitis, biofilm, virulence factor, antimicrobial susceptibility Mastitis is an inflammation of the mammary gland caused by several varieties of bacteria such as Staphylococcus (S.) aureus, S. epidermidis, and strep- † Equal contributors

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Alkasir

Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella

Lämmler¹,

Sammra²,

Glaeser³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

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True.pe.rel'la. N.L. fem. n. Trueperella named after Hans Georg Trüper, the German microbiologist. Actinobacteria / Actinobacteria / Actinomycetales / Actinomycetaceae / Trueperella Cells are Gram‐reaction‐positive, nonmotile, non‐spore‐forming coccobacilli and short rods that occur singly, in pairs (V, T, and palisade formations), or in clusters. Cells vary in shape and size in different media. Cells from 24‐h‐old broth cultures are Gram‐reaction‐positive but may be Gram‐reaction‐variable in older cultures. Pinpoint, in most cases β‐hemolytic colonies occur on sheep blood agar after 24 h of incubation. After 48–72 h of incubation, colonies are 0.5–1.5 mm in diameter, convex, circular, and translucent with entire edges. Aerobic and facultatively anaerobic. Strictly fermentative. Lactic acid is the primary metabolic product in glucose–yeast extract broth, but acetate and succinate are minor products. The peptidoglycan is of the type A5a, variations l ‐Lys– l ‐Ala– l ‐Lys– d ‐Glu or l ‐Lys– l ‐Lys– d ‐Glu. The muramic acid residue of the peptidoglycan is N ‐acetylated. Characteristic whole‐cell sugars are rhamnose and glucose. Mycolic acids are not present. The long‐chain fatty acids are primarily straight‐chain saturated and monounsaturated in addition to traces of branched‐chain fatty acids of the iso‐/anteiso type. The phospholipid type is type PI, comprising diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), and an unknown phosphoglycolipid (PGL) of unknown composition. The principal menaquinone is MK‐10(H4). DNA G + C content (mol%) : 56–66. Type species : Trueperella pyogenes Yassin et al. 2011 VP .

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Investigation of antimicrobial susceptibility and virulence factor genes in Trueperella pyogenes isolated from clinical mastitis cases of dairy cows

Tamai

Mohammadzadeh

Salehi

et al. 2021

Food Science & Nutrition

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Genotypic characterization and evaluation of an antibiotic resistance of Trueperella pyogenes (Arcanobacterium pyogenes) isolated from milk of dairy cows with clinical mastitis

Cited by 58 publications

References 21 publications

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Trueperella

Investigation of antimicrobial susceptibility and virulence factor genes in Trueperella pyogenes isolated from clinical mastitis cases of dairy cows

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