Investigation of antimicrobial susceptibility and virulence factor genes in <i>Trueperella pyogenes</i> isolated from clinical mastitis cases of dairy cows

Tamai, Iradj Ashrafi; Mohammadzadeh, Abdolmajid; Salehi, Taghi Zahraei; Mahmoodi, Pezhman; Pakbin, Babak

doi:10.1002/fsn3.2431

Cited by 14 publications

(11 citation statements)

References 19 publications

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“…In the female European bison in this study, the reproduction tract infections causing by T. pyogenes were noted mainly as suppurative lesions in the vagina or sporadically in the uterus, which is in accordance with previous findings [ 16 , 31 ] whereas, in cattle, T. pyogenes infections are mostly related to metritis , leading to impaired reproductive performance and milk production [ 17 , 18 , 24 , 32 ].…”

Section: Discussionsupporting

confidence: 92%

“…Nevertheless, among 36 samples obtained from forest musk deer ( Moschus berezovskii ) kept in captivity in China, T. pyogenes was isolated from all of these samples [ 21 ]. In cattle, the prevalence of T. pyogenes among isolates from mastitis was 13.75% [ 24 ]. Moreover, this bacterium was often isolated as a main etiological agent of the infections.…”

Section: Discussionmentioning

confidence: 99%

“…In that study, the percentage of occurrence of cbpA , nanH and nanP was 12%, 40% and 44%, respectively [ 16 ]. In contrast, these genes are often found in the vast majority of bovine T. pyogenes strains [ 17 , 24 , 42 , 43 ]. However, in some studies, they were detected in a lower number of T. pyogenes isolates from cattle [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…The comparable results, the highest presence of fimA , fimC and fimE , and lower of fimG , were also noted for T. pyogenes strains isolated from cattle in Poland [ 18 ]. Generally, a high frequency of fimbrial genes was detected in the bovine strains in most studies [ 17 , 24 , 42 , 43 ], as well as among strains isolated from white-tailed deer [ 44 , 45 ]. Nevertheless, it seems that fimbriae subunits A and E are involved in the pathogenicity of T. pyogenes strains causing infections in European bison.…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation of genetic diversity and relationships among bacterial strains is a very important aspect of epidemiological studies. So far, few molecular methods were applied for typing T. pyogenes strains isolated from various hosts [ 17 , 20 , 21 , 22 , 23 , 24 , 25 ]. However, the knowledge about the genetic diversity among T. pyogenes strains found in European bison is insufficient.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Genetic Diversity of Trueperella pyogenes Isolated from Infections in European Bison (Bison bonasus)

Kwiecień

Stefańska

Kizerwetter-Świda

et al. 2022

Animals

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Trueperella pyogenes is a Gram-positive bacterium causing purulent infections in many animal species, including the European bison. However, the data about the virulence and genetic relationships of T. pyogenes strains isolated from these wild ruminants are strongly limited. The aim of this study was to investigate the prevalence of T. pyogenes infections in the European bison, and to evaluate the genetic diversity of isolates from these animals. In the time span of10 years, 328 European bison from 16 different locations were examined. The standard bacteriological methods were used for T. pyogenes isolation and identification from clinical specimens obtained from urogenital tract infections and abscesses of different locations. The presence of genes encoding known virulence factors was investigated by PCR, and the genetic diversity of T. pyogenes strains was examined with the RAPD-PCR method. The prevalence of T. pyogenes infections was 14.6%, and the pathogen was isolated from both female (47.9% of isolates) and male (52.1% of isolates) European bison. It should be highlighted that a considerable number of strains were isolated from the prepuce and penis infections. Therefore, the role of T. pyogenes in the pathogenesis of balanoposthitis should be seriously perceived. A total of 39 T. pyogenes strains were subjected to genetic characterization. All studied strains carried the plo gene, while the nanH (25.6%), nanP (23.1%), cbpA (7.7%), fimA (97.4%), fimC (69.2%), fimE (92.3%) and fimG (15.4%) genes were present with a variable frequency among the tested strains. The virulence genotype plo/fimA/fimC/fimE was dominant. RAPD-PCR typing showed a high level of genetic diversity among European bison T. pyogenes strains, and a total of 31 different RAPD profiles were distinguished. In a few cases, the same RAPD profile was found in strains obtained from animals living in the same area. This study provided the first data about the prevalence and genetic relationships of T. pyogenes in the Polish population of European bison. However, further epidemiological investigations are needed to understand the routes of transmission and dissemination of the pathogen in these wild animals.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and Genetic Diversity of Trueperella pyogenes Isolated from Infections in European Bison (Bison bonasus)

Kwiecień

Stefańska

Kizerwetter-Świda

et al. 2022

Animals

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Construction of live-attenuated Trueperella pyogenes by antibiotic treatment and sequential passage: methods for vaccine development

2023

Self Cite

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Trueperella pyogenes ( T. pyogenes ) is a zoonotic pathogen that is cause a variety of pyogenic diseases in animals. The complex pathogenicity and various virulence factors are important challenges to produce an effective vaccine. According to previous trials, inactivated whole-cell bacteria or recombinant vaccines were unsuccessful in preventing disease. Thus, this study aims to introduce a new vaccine candidate based on a live-attenuated platform. For this purpose, first T. pyogenes was subjected to sequential passage (SP) and antibiotic treatment (AT) to lose their pathogenicity. Second, Plo and fimA expressions as virulence genes were evaluated by qPCR and then mice were challenged with bacteria from SP and AT culture by intraperitoneal route. Compared to the control group ( T. pyogenes -wild type), plo and fimA gene expressions were downregulated and vaccinated mice have a normal spleen appearance in contrast to the control group. In addition, there was no significant difference between bacterial count from spleen, liver, heart and peritoneal fluid in vaccinated mice and the control group. In conclusion, this study introduces a new T. pyogenes vaccine candidate based on a live-attenuated strategy that mimics natural infection without pathogenicity for further investigation on vaccines against T. pyogenes infections. Graphical abstract

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Immunoinformatics design of novel multi-epitope vaccine against Trueperella Pyogenes using collagen adhesion protein, fimbriae, and pyolysin

Beikzadeh

2024

Arch Microbiol

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Investigation of antimicrobial susceptibility and virulence factor genes in Trueperella pyogenes isolated from clinical mastitis cases of dairy cows

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 14 publications

References 19 publications

Prevalence and Genetic Diversity of Trueperella pyogenes Isolated from Infections in European Bison (Bison bonasus)

Prevalence and Genetic Diversity of Trueperella pyogenes Isolated from Infections in European Bison (Bison bonasus)

Construction of live-attenuated Trueperella pyogenes by antibiotic treatment and sequential passage: methods for vaccine development

Immunoinformatics design of novel multi-epitope vaccine against Trueperella Pyogenes using collagen adhesion protein, fimbriae, and pyolysin

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