Abstract:Summary Seeds of Capsicum annuum L. cv. Pusa jwala and G 4 were treated with varying concentrations of methyl methane sulphonate (MMS), and the effect of various concentrations of MMS on each stage of meiosis has been studied in M1 generation. Various types of meiotic abnormalities and reduction in chiasma frequency were observed in treated populations. However, MMS treatment were found to be more effective in inducing meiotic abnormalities and reduction in chiasma frequency in Pusa jwala as compared to G 4 . … Show more
“…The fact that no significant increase in chromosome lagging and chromosome aberrations was seen in periods later than those observed, at concentrations of 10 and 15 mg/L in accession GF004 of bean (p ≥ 0.05), may be explained as being due to mitotic inhibition for an exposure period of 24 h, because the chromosome aberrations evaluated in this study require cells that are undergoing division to be noticed ( Rank, 2003 ). MMS is a clastogenic agent, and thus promotes chromosome breaks and the induction of chromosome lagging in plants, as previously reported in Capsicum annum L. ( Gulfishan et al , 2011 ; 2012 ) and Vicia faba L. ( Sharma et al , 2009 ) and also observed in the present study. In accession GF007, MMS did not produce a significant increase in the frequency of chromosome aberrations in the meristematic root cells, when compared with the control ( Table 4 ).…”
This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used.
“…The fact that no significant increase in chromosome lagging and chromosome aberrations was seen in periods later than those observed, at concentrations of 10 and 15 mg/L in accession GF004 of bean (p ≥ 0.05), may be explained as being due to mitotic inhibition for an exposure period of 24 h, because the chromosome aberrations evaluated in this study require cells that are undergoing division to be noticed ( Rank, 2003 ). MMS is a clastogenic agent, and thus promotes chromosome breaks and the induction of chromosome lagging in plants, as previously reported in Capsicum annum L. ( Gulfishan et al , 2011 ; 2012 ) and Vicia faba L. ( Sharma et al , 2009 ) and also observed in the present study. In accession GF007, MMS did not produce a significant increase in the frequency of chromosome aberrations in the meristematic root cells, when compared with the control ( Table 4 ).…”
This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used.
“…In this investigation, pollen fertility decreased with the increase in concentrations of the 5-BU. Similar decrease in pollen fertility was reported earlier in Vicia faba , Capsicum annuum (Gulfishan et al, 2010) and C. intybus . Reduction in pollen fertility also supports a decrease in seed production due to the meiotic anomalies.…”
This work was done to study the effect of base analogue 5-bromouracil (5-BU) on the medicinal herb, Cichorium intybus. 5-BU induced miss pairing during the DNA replication. The seeds of C. intybus were treated with different concentrations of 5-BU. Variations in some parameters such as seed germination, seedling survival, seedling height, pollen fertility, days to flowering, days to maturity, number of leaves per plant, plant height, and chromosome behavior were studied in M1 generation. A positive correlation between increasing concentrations of mutagen and various cytomorphological characters of C. intybus was observed.
“…Jayabalan and Rao (1987) suggested that stickiness might be due to disturbances in the cytochemically balanced reaction. Precocious movement of chromosome as observed during the present investigation, probably caused by spindle disfunction (Gulfishan et al 2010). Non-orientation and scattering of chromosomes at metaphase I was observed in the present investigation which may be due to either the inhibition of spindle formation or the destruction of spindle fibers (Kumar and Rai 2007).…”
Cytogenetical investigations have been carried out on Hordeum vulgare. Seed of H. vulgare were treated with different concentrations (0.1, 0.2, 0.3, 0.4 and 0.5%) of ethyl methane sulphonate (EMS) and hydrazine hydrate (HZ). The plants in the treated populations showed varying degrees of meiotic irregularities almost at all the mutagenic concentrations. The frequency of meiotic irregularities was found to be more in HZ treatment than EMS. The various types of meiotic abnormalities such as univalents, multivalents, stickiness, precocious movement, stray bivalent, non-orientation, cytomixis, laggard, bridges, unequal separation, micronuclei and disturbed polarities were observed. Taking the percentage of meiotic abnormalities and pollen sterility as an index of effectiveness of a mutagen, HZ treatment was found to be more effective.
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