Seeds of Capsicum annuum L. var. G4 were subjected to different concentrations of methyl methane sulphonate (MMS) and diethyl sulphate (DES). The effects of different mutagenic treatments on meiosis, chiasma frequency, and pollen fertility have been studied in M1 generation. Various types of meiotic aberrations such as univalent, multivalent, stickiness, bridge, laggards, cytomixis etc. were observed in all the treatments. However, the MMS treatments proved to be more effective in inducing meiotic aberrations as compared to DES. Moreover, the frequency of meiotic aberrations was at its maximum at metaphase followed by anaphase and telophase stages. As the concentrations increase, reduction in chiasma frequency and pollen fertility was observed in all the treatments and, MMS again was found to be more effective than DES treatments.
Seeds of Capsicum annuum L. var. Pusa jwala and G4 were treated with varying concentrations (0.01% -to-0.05%) of diethyl sulphate (DES) and their effect on different stages of meiosis studied in M 1 generation. Various types of meiotic abnormalities and reduction in chiasma frequency were noticed in the diethyl sulphate treated populations. The highest percentage of chromosomal aberration (22.66% in var. G4 and 20.45% in var. pusa jwala) was recorded at the highest concentration (0.05% DES) of the mutagen. However, DES treatments were found to be more effective in inducing meiotic abnormalities and reduction in chiasma frequency in var. G4 (1.20) as compared to var. Pusa jwala (1.30). Further, a negative relationship between increasing concentrations of DES and reduction in chiasma frequency in treated population was also observed. Taking the percentage of meiotic abnormalities and reduction in chiasma frequency as an index of sensitivity of genotype to DES, var. G4 was found to be more sensitive than var. Pusa jwala.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.