RESUMO-As frutas nativas do cerrado têm despertado ultimamente interesse crescente, devido às suas propriedades nutricionais e funcionais aliadas ao potencial para agregar valor e conservar a biodiversidade deste bioma. Muitos compostos fenólicos apresentam capacidade antioxidante de neutralizar a atividade de radicais livres gerados no organismo, que estão associados a diversas doenças crônico-degenerativas. O objetivo deste trabalho foi avaliar a eficiência de três soluções extratoras (acetona a 70 %, etanol a 95 % e metanol a 99,8 %) e determinar os teores de compostos fenólicos em 10 espécies de frutas nativas do cerrado, utilizando o método de Folin-Ciocalteou para compostos fenólicos totais e o método da vanilina para taninos condensados. A acetona a 70 % mostrou-se como o melhor solvente extrator de compostos fenólicos totais e taninos condensados em frutos de Pouteria gardneriana, Eugenia dysenterica, E. klostzchiana, E. punicifolia, Plinia edulis, Campomanesia sp., fenólicos totais de Brosimium gaudichaudii e taninos condensados de Jaracatia spinosa. O etanol a 95 % foi mais eficiente na extração de fenólicos totais de Jaracatia spinosa e taninos condensados de Brosimium gaudichaudii; o metanol a 99,8 % foi mais eficiente na extração de taninos condensados de Eugenia dysenterica. Os teores de compostos fenólicos totais variaram entre 90 e 327 mg de ácido gálico equivalente por 100g de polpa para as espécies E. dysenterica e E. punicifolia, respectivamente. Os teores de taninos condensados variaram entre 4 e 291 mg de catequina equivalente por 100 g de polpa para as espécies E. dysenterica e E. calcyna, respectivamente. As espécies de frutas do cerrado, avaliadas neste estudo, podem ser consideradas boas fontes de compostos fenólicos totais, sendo que a natureza específica dos diferentes tipos deve ser avaliada em estudos futuros. Termos para indexação: Anacardium spp., Eugenia spp., Brosimium gaudichaudii, Campomanesia sp., Pouteria gardneriana, Plinia edulis, Jaracatia spinosa . TOTAL PHENOLICS AND CONDENSED TANNINS IN NATIVE FRUITS FROM BRAZILIAN SAVANNAABSTRACT -The native fruits from Brazilian savanna are arousing increasing interest due to their nutritional and functional properties combined with the potential to add value and conserve the biodiversity of this biome. Many phenolic compounds have antioxidant capacity to neutralize free radicals generated in the body that are associated with many chronic degenerative diseases. The objective of this study was to evaluate the efficiency of three extraction solutions (acetone 70%, ethanol 95% and methanol 99.8 %) and determining the levels of phenolic compounds in 10 species of native fruits from Brazilian savanna, using the Folin-Ciocalteu method for total phenolic compounds and vanillin method for condensed tannins. Acetone 70 % proved to be the best solvent extractor for total phenolics and condensed tannins in fruits of Pouteria gardneriana, Eugenia dysenterica, E. klostzchiana, E. punicifolia, Plinia edulis, Campomanesia sp. and for total phenolics...
Copper compounds have been intentionally introduced into water bodies as aquatic plant herbicides, algicides and molluscicides. Copper-based fertilizers and fungicides have been widely used in agriculture as well. Despite the fact that copper is an essential element for all biota, elevated concentrations of this metal have been shown to affect a variety of aquatic organisms. Nonetheless, comparative studies on the susceptibility of different freshwater species to copper compounds have seldom been performed. This study was conducted to compare toxicity of copper-based pesticides (copper oxychloride, cuprous oxide and copper sulfate) to different freshwater target (Raphidocelis subcapitata, a planktonic alga and Biomphalaria glabrata, a snail) and non-target (Daphnia similis, a planktonic crustacean and Danio rerio, a fish) organisms. Test water parameters were as follows: pH = 7.4 +/- 0.1; hardness 44 +/- 1 mg/l as CaCO3; DO 8-9 mg/l at the beginning and > 4 mg/l at the end; temperature, fish and snails 25 +/- 1 degrees C, Daphnia 20 +/- 2 degrees C, algae 24 +/- 1 degrees C. D. similis (immobilization), 48-h EC50s (95% CLs) ranging from 0.013 (0.011-0.016) to 0.043 (0.033-0.057) mg Cu/l, and R. subcapitata (growth inhibition), 96-h IC50s from 0.071 (0.045-0.099) to 0.137 (0.090-0.174) mg Cu/l, were the most susceptible species. B. glabrata (lethality), 48-h LC50s from 0.179 (0.102-0.270) to 0.854 (0.553-1.457) mg Cu/l, and D. rerio (lethality), 48-h LC50s 0.063 (0.045-0.089), 0.192 (0.133-0.272) and 0.714 (0.494-1.016) mg Cu/l, were less susceptible than Daphnia to copper-based pesticides. Findings from the present study therefore suggest that increased levels of copper in water bodies is likely to adversely affect a variety of aquatic species.
Arachis hypogaea , known as the peanut, is native to South America. Peanut contains several active components including flavonoids, phenolic acids, phytosterols, alkaloids, and stilbenes. Some therapeutic effects have been reported for peanut seed extracts, such as antioxidative, antibacterial, antifungal, and anti-inflammatory activities. This paper aims to give an overview of the chemical composition, focusing on secondary metabolites, and of the biological activity of A. hypogaea, to stimulate new studies about species of the Arachis genus.
Dentre as fruteiras do Cerrado brasileiro com forte potencial para a exploração sustentada, encontram-se o araticum (Annona Crassiflora Mart.), o coquinho-azedo (Butia Capitata Mart.) e o pequi (Caryocar Brasiliense Camb.). O objetivo deste trabalho foi caracterizar o teor de óleo e o perfil de ésteres metílicos da fração lipídica da polpa dos frutos destas três espécies. Os teores de lipídeos foram determinados por extração contínua a quente com éter de petróleo em extrator tipo Soxhlet. O óleo para perfil de ésteres metílicos foi extraído a frio por Bligh e Dyer e caracterizado por cromatografia a gás, usando detector de ionização de chama. A polpa de pequi apresentou elevados teores de óleo, em média 30,89 %; as polpas de araticum e coquinho-azedo apresentaram, respectivamente, médias de 2,14 e 2,73 % de óleo. Os ácidos graxos oleico e palmítico predominaram nas três espécies, e todas apresentaram prevalência de ácidos graxos insaturados, sendo a maior concentração encontrada no araticum (78,3 %), seguida pelo coquinho-azedo (63,3 %). A polpa de araticum e de coquinho-azedo apresentaram elevados teores de ácido linolênico (2,5 a 3,7%). A presença de ésteres metílicos de ácido caproico parece estar associada à percepção do aroma frutal típico destas frutas do Cerrado.
Peanut is one of the few plants that synthesizes resveratrol, a phenolic compound of the stilbene class, which has been associated with reduced risk of developing chronic diseases, such as cancer, cardiovascular diseases, skin diseases, pulmonary diseases, diabetes and neurological diseases. Resveratrol was detected in different parts of the peanut plant, including roots, leaves, seeds and their derivatives. The wild species of the Arachis section are also strong candidates to synthesize resveratrol because they are phylogenetically closely related to cultivated peanut. Our objective was to characterize the resveratrol content in ten wild species of Arachis with three different genomes (A, B and K). The plant material was composed of leaves of the ten species treated (test) and not treated (control) with ultraviolet (UV) radiation. The test and control samples were extracted and the identification and quantification of resveratrol was performed using high performance liquid chromatography (HPLC). All species studied synthesized resveratrol and the concentrations ranged from 299.5 lg/g in A. kempff-mercadoi to 819.9 lg/g in A. cardenasii. DPPH antioxidant activity varied between 18.7 % for A. duranensis and 48.2 % in A. simpsonii. The results showed that wild Arachis species are a potential source of alleles for improvement of cultivated peanut, with the aim of achieving higher resveratrol content in leaves. Keywords Antioxidant Á Fabaceae Á Peanut Á Stilbene Á Wild Arachis Introduction Peanut is one of the few plants that synthesize resveratrol (3, 5, 4 0-trihidroxistilbene), a phenolic compound of the stilbene class. This bioactive compound has been associated with reduced risk of developing several chronic diseases, such as cancer, cardiovascular diseases, skin diseases, pulmonary diseases, diabetes and neurological diseases (Harikumar and Aggarwal 2008;
Problem-based learning (PBL) has been systematized for over forty years. However, there is still a lack of studies that seek to visualize the evolution of scientific literature on the subject. This paper seeks to fill this gap through the development of a bibliometric analysis by carrying out a data search on the Web of Science™ database and subsequently transferring the acquired data for treatment to the VantagePoint © software package. The data indicate that research on PBL shows a strong progression since the end of the twentieth century, with a high volume of the most impacting studies being carried out by a group of researchers who are currently linked to or used to be connected to the Maastricht University. As a result of these studies, a geographical (from North America and Europe to Asia) and academic (from research in medical education to other biomedical and engineering areas) spread of scientific production regarding this teaching methodology has arisen.
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