Genome sequence of Rhizomucor pusillus FCH 5.7, a thermophilic zygomycete involved in plant biomass degradation harbouring putative GH9 endoglucanases

Hüttner, Silvia; Nguyen, Thanh Thuy; Pelt, Sake van; Larsbrink, Johan; Thanh, Vu Nguyen; Olsson, Lisbeth

doi:10.1016/j.btre.2018.e00279

Cited by 13 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4). These species are not known as strong lignocellulose degraders and their genomes contain only about a half of the number of the CAZymes identified in the lignocellulolytic fungus Thielavia terrestris 28,29 . These fast-growing Mucoraceae are widespread in nature; they are often the first fungi to occupy moist plant debris 30 and are known for their ability to degrade starch, proteins and lipids, which are much less recalcitrant substrates compared to lignocellulose.…”

Section: Resultsmentioning

confidence: 99%

Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity

Thanh¹,

Thuy²,

Huong³

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Thermophilic fungi can represent a rich source of industrially relevant enzymes. Here, 105 fungal strains capable of growing at 50 °C and pH 2.0 were isolated from compost and decaying plant matter. Maximum growth temperatures of the strains were in the range 50 °C to 60 °C. Sequencing of the internal transcribed spacer (ITS) regions indicated that 78 fungi belonged to 12 species of Ascomycota and 3 species of Zygomycota, while no fungus of Basidiomycota was detected. The remaining 27 strains could not be reliably assigned to any known species. Phylogenetically, they belonged to the genus Thielavia , but they represented 23 highly divergent genetic groups different from each other and from the closest known species by 12 to 152 nucleotides in the ITS region. Fungal secretomes of all 105 strains produced during growth on untreated rice straw were studied for lignocellulolytic activity at different pH and temperatures. The endoglucanase and xylanase activities differed substantially between the different species and strains, but in general, the enzymes produced by the novel Thielavia spp. strains exhibited both higher thermal stability and tolerance to acidic conditions. The study highlights the vast potential of an untapped diversity of thermophilic fungi in the tropics.

show abstract

Section: Resultsmentioning

confidence: 99%

Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity

Thanh¹,

Thuy²,

Huong³

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thielavia terrestris, (Garcia-Huante et al 2017), Talaromyces thermophilus (Maalej et al 2009), Paecilomyces thermophile (Fan et al 2012), Achaetomium sp. X2-8 (Chadha et al 2019), Rhizomucor pusillus (Hüttner et al 2018), Rasamsonia emersonii, (Martínez et al 2016) T. Leycettanus (Wang et al 2017), Melanocarpus albomyces (Gupta et al 2013) and Aspergillus oryzae LC1 (Bhardwaj et al 2019) were found to be producer of hyper-thermophilic active xylanase. Several alkali stable xylanases were obtained from different fungal strains such as Paenibacillus barcinonensis (Valenzuela et al 2010), Aspergillus fumigatus MA28 (Bajaj and Abbass 2011), Cladosporium oxysporum (Guan et al 2016) and Aspergillus oryzae LC1 (Bhardwaj et al 2019).…”

Section: Fungal Sources Of Xylanasesmentioning

confidence: 99%

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj

Kumar

Verma

2019

Bioresour. Bioprocess.

277

105

View full text Add to dashboard Cite

Xylan is the second most abundant naturally occurring renewable polysaccharide available on earth. It is a complex heteropolysaccharide consisting of different monosaccharides such as l-arabinose, d-galactose, d-mannoses and organic acids such as acetic acid, ferulic acid, glucuronic acid interwoven together with help of glycosidic and ester bonds. The breakdown of xylan is restricted due to its heterogeneous nature and it can be overcome by xylanases which are capable of cleaving the heterogeneous β-1,4-glycoside linkage. Xylanases are abundantly present in nature (e.g., molluscs, insects and microorganisms) and several microorganisms such as bacteria, fungi, yeast, and algae are used extensively for its production. Microbial xylanases show varying substrate specificities and biochemical properties which makes it suitable for various applications in industrial and biotechnological sectors. The suitability of xylanases for its application in food and feed, paper and pulp, textile, pharmaceuticals, and lignocellulosic biorefinery has led to an increase in demand of xylanases globally. The present review gives an insight of using microbial xylanases as an "Emerging Green Tool" along with its current status and future prospective.

show abstract

“…Different thermophilic fungi such as Thielavia terrestris ( García-Huante et al, 2017 ) , Rhizomucor pusillus ( Hüttner et al, 2018 ) , and Corynascus thermophiles ( van den Brink et al, 2013 ) have been reported as very good sources of thermostable xylanase. It was recently shown that Thielavia terrestris Co3Bag1 produces a highly thermostable xylanase with a molecular weight of 82 kDa.…”

Section: Cellulase and Xylanase Of Thermophilesmentioning

confidence: 99%

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Ajeje¹,

Hu²,

Song³

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The bioconversion of lignocellulose into monosaccharides is critical for ensuring the continual manufacturing of biofuels and value-added bioproducts. Enzymatic degradation, which has a high yield, low energy consumption, and enhanced selectivity, could be the most efficient and environmentally friendly technique for converting complex lignocellulose polymers to fermentable monosaccharides, and it is expected to make cellulases and xylanases the most demanded industrial enzymes. The widespread nature of thermophilic microorganisms allows them to proliferate on a variety of substrates and release substantial quantities of cellulases and xylanases, which makes them a great source of thermostable enzymes. The most significant breakthrough of lignocellulolytic enzymes lies in lignocellulose-deconstruction by enzymatic depolymerization of holocellulose into simple monosaccharides. However, commercially valuable thermostable cellulases and xylanases are challenging to produce in high enough quantities. Thus, the present review aims at giving an overview of the most recent thermostable cellulases and xylanases isolated from thermophilic and hyperthermophilic microbes. The emphasis is on recent advancements in manufacturing these enzymes in other mesophilic host and enhancement of catalytic activity as well as thermostability of thermophilic cellulases and xylanases, using genetic engineering as a promising and efficient technology for its economic production. Additionally, the biotechnological applications of thermostable cellulases and xylanases of thermophiles were also discussed.

show abstract

Genome sequence of Rhizomucor pusillus FCH 5.7, a thermophilic zygomycete involved in plant biomass degradation harbouring putative GH9 endoglucanases

Cited by 13 publications

References 24 publications

Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity

Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Contact Info

Product

Resources

About