Genome analysis of species 3 adenoviruses isolated during summer outbreaks of conjunctivitis and pharyngoconjunctival fever in the glasgow and London areas in 1981

Abstract

Genome analysis was performed on 125 adenovirus isolates from conjunctival swabs of patients with conjunctivitis obtained in Glasgow between 1981 and 1984. A summer outbreak in 1981 was mainly due to species 3 adenoviruses, of which genotype 3GB and five different genotypic variants cocirculated. Three species 3 variants were also observed in 1982. The genome changes of variants were located on physical maps of the Ad3 reference strain and found to be clustered near the ends of the adenovirus DNA (including the fiber area), whereas the hexon coding region was unaltered. In contrast to the genome heterogeneity observed among the species 3 adenoviruses collected in Glasgow in 1981 it was found that all 69 Ad3 isolates obtained from an outbreak of pharyngoconjunctival fever in a boarding school near London during the summer of 1981 possessed the 3GB genotype.

Genomic Comparison of Adenovirus Type 3 Isolates from Patients with Acute Conjunctivitis in Japan, Australia, and the Philippines

“…Restriction patterns with BamHI and BglII of the isolates belonging to the former group (lanes a, b) corresponded to those of Ad3p (18,23). The restriction pattern of the isolates belonging to the latter group (lanes c-g) with BglII resembled that of Ad3d reported by O'Donnell et al (18). However, the H fragment of the latter group migrated slightly faster than that of Ad3p which shared the size of the H fragment with Ad3d.…”

“…No difference was found in the restriction pattern with HindIII among all Ad3 isolates. With SmaI, the restriction pattern diverged into two groups corresponding to Ad3p (lanes a, b) and Ad3a (lanes c-g) reported by O'Donnell et al (18). Restriction patterns with BamHI and BglII of the isolates belonging to the former group (lanes a, b) corresponded to those of Ad3p (18,23).…”

“…With SmaI, the restriction pattern diverged into two groups corresponding to Ad3p (lanes a, b) and Ad3a (lanes c-g) reported by O'Donnell et al (18). Restriction patterns with BamHI and BglII of the isolates belonging to the former group (lanes a, b) corresponded to those of Ad3p (18,23). The restriction pattern of the isolates belonging to the latter group (lanes c-g) with BglII resembled that of Ad3d reported by O'Donnell et al (18).…”

“…2). Details of changes in DNA fragment migration patterns after digestion with restriction endonucleases are summarized in Table 2 in which the data of O'Donnell et al (18) were compiled to show the relationships with other Ad3 genome types.…”

“…In Ad3, Wadell (23) recognized two genome types, Ad3p and Ad3a, and O'Donnell et al (18) recognized four additional genome types, Ad3b, Ad3c, Ad3d, and Ad3e by using a restriction endonuclease BglII. Furthermore, Bailey and Richmond (3) recognized 6 additional genome types, Ad3ai, Ad3a2, Ad3a3, Ad3a4, Ad3a5, and Ad3b1 by the combination of three restriction endonucleases, HindIII, PstI, and Smal.…”