Two new genome types of adenovirus 7c

Abstract

Adenovirus type 7 is the type most frequently associated with serious disease. Eighteen different genome types of adenovirus type 7 had been reported up to October 1986. The genome type Ad7c, based on the restriction enzyme profiles of SmaI and BamHI, has been reported from Europe prior to 1969 and more recently from South Africa. Here, we report two new genome types of adenovirus 7 c that have not previously been identified and that have been isolated in South Africa between 1975 and 1986 from children with postmeasles pneumonia. The two new genome types differ from the prototype Ad7c virus in having two (Ad7c1) or one (Ad7c2) extra cleavage sites for the restriction endonuclease EcoRI. These sites have been located at 3.68kb and 5.32kb from the left terminus of the genome map published for the prototype Ad7c strain. A strain resembling the prototype Ad7c was also isolated in 1986 from a case of post measles pneumonia.

Molecular epidemiology of adenovirus types 3 and 7 isolated from children with pneumonia in Beijing

“…A nomenclature system has been suggested [Li and Wadell, 19861 and applied for the description of many genome types at different laboratories [Arens and Worth, 1988;Fu et al, 1989;Guo et al, 1988;Kannemeyer et al, 1988;Golovina et al, 19911. We now describe it in detail with a minor modification.…”

“…investigate the genomic variability of the Ad3 and Ad7 genome types (GTs) and to evaluate their relative virulence [Wadell et al, 1985;Wadell, 1984;Wadell and Varsanyi, 1978;Bailey and Richmond, 1986;O'Donnell et al, 1986;Arens and Worth, 1988;Guo et al, 1988;Kannemeyer et al, 1988;Adrian et al, 1989;Itakura et al, 1990;Golovina et al, 1991;Kajon and Wadell, 19941. Using 12 REs, 17 GTs of Ad3 and 15 GTs of Ad7 have been identified [Li and Wadell 1986, 19881.…”

Adenovirus type 7 infections in children in new South Wales, Australia

“…Since the isolation of the prototype strain Gomen of Ad7 (Ad 7p) by Berget et al [19551, a total of 15 Ad7 genome types designated Ad7p, Ad7a, Ad7al to Ad7a5, Ad7b, Ad7c, Ad7d, Ad7d1, Ad7e, Ad7f, and Ad7g have been described by Li and Wadell [19861 and their distribution worldwide reported recently [Wadell et al, 19851. Overall, during the last decade, Ad7b predominated in Australia, Europe, and North America and Ad7c was detected in South Africa, Ad7d in China, Ad7e in Brazil, and Ad7f in Australia [Wadell et al, 1985;Kannemeyer et al, 1988;Li and Wadell, 19861. The present report describes our own experience of infection due to Ad7 in New South Wales, Australia. It confirms the above-mentioned data relating to Ad7b [Sutton et al, 1976;Straube et al, 1983;Wadell et al, 1981 I, thus emphasising the importance of this genome type in epidemic Ad7 infection in Australian children as well.…”

Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of S�o Paulo, Brazil

“…EcoT14I and HindIII digests of lambda DNA (Takara Shuzo Co.) and a 1-kb DNA ladder (Gibco BRL) were run as molecular weight standard. Subgenus and serotype identifications were undertaken by comparison of the resulting patterns with the published restriction patterns of the prototypes and genome types [Fujii et al, 1983[Fujii et al, , 1984Adrian et al, 1986;Bailey and Richmond, 1986;Li and Wadell, 1986;Kannemeyer et al, 1988;Adrian et al, 1990;Golovina et al, 1991;Niel et al, 1991;De Jong et al, 1992;Azar et al, 1998]. …”

“…Serotype 7 is associated with respiratory and ocular diseases, showing great genetic variability. Twenty-six genome types of Ad7 have been identified, including the genome type Ad7h, from cases of severe lower respiratory tract diseases isolated in Argentina, Chile, and Uruguay [Wadell et al, 1985;Bailey and Richmond, 1986;Li and Wadell, 1986;Kannemeyer et al, 1988;Golovina et al, 1991;Niel et al, 1991;Azar et al, 1998]. Serotype 8, isolated for the first time in 1955, is the original causative agent of epidemic keratoconjunctivitis [Jawetz et al, 1955].…”