Molecular epidemiology of adenovirus types 3 and 7 isolated from children with pneumonia in Beijing

Abstract

One hundred fifty strains of adenovirus sero-types 3 (Ad3) and 7 (Ad7) were analyzed. The viruses were isolated from patients, the majority of whom had pneumonia, from central and sub-urban Beijing over a 33-year period (1958-1990). Genomic analysis of DNA extracted from 74 strains of Ad3 and 76 strains of Ad7, with four to five restriction endonucleases (REs), revealed the presence of four and eight genome types, respectively: Ad3a2, Ad3a4, Ad3a5, Ad3a6 and Ad7p1, Ad7a1, Ad7a4, Ad7b, Ad7b1, Ad7d, Ad7d1, and Ad7g. Ad7b1 was the most recently identified genome type. The restriction patterns obtained from 19 representatives of Ad7 genome types after cleavage of the DNA with 12 REs are shown. Ad3a2 first appeared in 1962, and predominated from 1983 to 1988. Ad3a4 was the main causative agent of pneumonia in 1982. Ad3a2 and Ad3a4 are closely related and have 97% pairwise comigrating restriction fragments (PCRF). Ad7d predominated over a period of 11 years (1980-1990). It has 98% PCRF with Ad7b. Ten pairs of strains isolated from different specimens of the same patients were all concordant.

Detection of three human adenovirus species in adults with acute respiratory infection in China

“…Our results are similar to those of a previous study among children in China [10], in which HAdV-3 and HAdV-7 were the most identified serotypes. These results are also consistent with data from the United Kingdom [20], Canada [26], Korea [23], and Taiwan, China [27], in which species B was the most commonly identified HAdV.…”

“…However, incidences of adenovirus-associated illnesses are higher among children than among adults. As such, in China, the impact of HAdVs on respiratory infection is well characterized in children [10][11][12][13][14][15] but not in adults.…”

Ten-year analysis of adenovirus type 7 molecular epidemiology in Korea, 1995–2004: Implication of fiber diversity☆

“…Similarly, Ad7h was the predominant genome type in the south cone of South America where it has circulated almost as the sole genomic variant of Ad7 since 1986 after substituting for genome type Ad7c (Kajon and Wadell, 1994;Kajon et al, 1996). In China, one genome type, Ad7d predominated for 10 years, between 1980and 1990(Li et al, 1996. In contrast, our results demonstrate that the prevalence of the Ad7d and Ad7l genome types alternated in Korea over the 10-year period between 1995 and 2004.…”

“…Various genome types and subtypes circulate in different countries and in different time period (Carballal et al, 2002;Gray et al, 2005;Kajon et al, 1996;Li et al, 1996). Adenoviruses with diverse genome types can cause outbreaks with various degrees of severity.…”

“…Of the 51 serotypes, adenovirus type 7 (Ad7) is most frequently associated with a severe clinical outcome and high fatality, especially in young infants and immunocompromised hosts (Hong et al, 2001;Li et al, 1996;Noda et al, 2002;Yang and Rubin, 1995). Restriction analysis of genomic DNA remains the most popular method of describing Ad7 genome types.…”