Genetic variability and heterozygosity of cocoa accessions of Parinari (Pa) population, based on microsatellite markers

Yamada, Mamoru; Faleiro, Fábio Gelape; Pires, José Luís; Neto, A. Dantas; Flores, Acassi Batista; Faleiro, A. S. G.

doi:10.12702/1984-7033.v03n04a07

Cited by 3 publications

(5 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This number is higher than the 5.6 alleles/locus found by Risterucci et al (2000) or 3.7 alleles/locus found by Yamada et al (2003) using agarose gel, indicating the high variability in the parent accessions used here (Figure1) The genetic distance ranged from 0, 17 to 0.90 (data not shown) and shows great dispersion of accessions (Figure 2), also indicating high variability.…”

Section: Resultscontrasting

confidence: 53%

“…The heterozygosity ranged from 20% to 86% (Table1), and in 79% more than 50% loci were heterozygous. This value is high, compared with other reports (Faleiro et al 2004, Yamada et al 2003, Bertolde 2007. Clone ICS 95 was one of the least, and EET 62 and TSH 1188 were the most heterozygous.…”

Section: Resultscontrasting

confidence: 53%

“…In farm selections Faleiro et al (2004) found that 50% of the clones had over 50% heterozygous loci. In the Parinari population, Yamada et al (2003) found a heterozygosity of over 50% in about 40% of the accessions. The heterozygosity of 38 recommended genotypes ranged from 26 to 92% (Bertolde 2007).…”

Section: Introductionmentioning

confidence: 91%

See 2 more Smart Citations

Microsatellite diversity and heterozygosity of parents of a cocoa breeding population

Yamada¹,

Faleiro²,

Flores³

et al. 2009

CBAB

Self Cite

View full text Add to dashboard Cite

-The purpose of this study was to determine the genetic diversity and heterozygosity of 26 clones used as parents of 27 families. The populations are being evaluated by the Comissão Executiva do Plano da Lavoura Cacaueira (CEPLAC), in the state of BA. Nine of these clones are currently being recommended to farmers, while six others were used as control. The seven microsatellite generated 52 alleles with a mean of seven alleles/locus and genetic distance ranging from 0.17 to 0.90. This indicates a wide distribution of accessions and high variability. The heterozygosity ranged from 20% to 86%, and more than 50% of the loci were heterozygous in 79% of the clones. Although the selection of the parents for populations was not based on genetic distances, the high genetic diversity and heterozygosity of parents indicate highly segregating populations that make the selection of trees of interest possible, due to the variability.

show abstract

Section: Resultscontrasting

confidence: 53%

Section: Resultscontrasting

confidence: 53%

See 1 more Smart Citation

Microsatellite diversity and heterozygosity of parents of a cocoa breeding population

Yamada¹,

Faleiro²,

Flores³

et al. 2009

CBAB

Self Cite

View full text Add to dashboard Cite

show abstract

“…This predominance of monomorphic bands (67% of the amplified bands) is consistent with the genetic diversity usually reported for T. cacao (Marita et al, 2001;Leal et al, 2008). The genetic similarity coefficient between the two genotypes of the Pa series was 0.67, the highest value was found by Yamada et al (2002Yamada et al ( , 2003 for genotypes of this series, based on microsatellite markers.…”

Section: Resultsmentioning

confidence: 51%

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa

Rehem

Almeida²,

Corrêa³

et al. 2010

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 SSR and 64 RAPD primers. The SSR primers amplified 65 RAPD primers, giving 179 polymorphic bands. After screening with SSR and RAPD markers, we selected 20 SSR primers, two SSR primers with ESTs and 22 RAPD primers that were polymorphic for genitors Pa 30 and Pa 169. Only two of the 22 RAPD primers and three of the 20 SSR primers were informative and polymorphic in the analysis of the bulk samples of progenies. Among these, primer RAPD E11 produced a band linked to the lethal gene (38.5 cM); none of the SSRs were associated with 'Luteus-Pa'.

show abstract

“…Molecular markers can be very useful in this task. The level of homozygosis has been determined for many clones (Mota 2003;Yamada et al 2003;Faleiro et al 2004b,c) and, in CEPLAC, progenies have already been produced considering this information.…”

Section: Heterozygosis Of Parentsmentioning

confidence: 99%

Genetic Improvement in Cocoa

Monteiro

Lopes²,

Clément³

Breeding Plantation Tree Crops: Tropical Species

View full text Add to dashboard Cite

Genetic variability and heterozygosity of cocoa accessions of Parinari (Pa) population, based on microsatellite markers

Cited by 3 publications

References 2 publications

Microsatellite diversity and heterozygosity of parents of a cocoa breeding population

Microsatellite diversity and heterozygosity of parents of a cocoa breeding population

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa

Genetic Improvement in Cocoa

Contact Info

Product

Resources

About