Genetic Relationships in Japanese Plum Cultivars by Molecular Markers

Bellini, E.; Giordani, Edgardo; Nencetti, V.; Paffetti, Donatella

doi:10.17660/actahortic.1998.478.6

Cited by 7 publications

(5 citation statements)

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“…Polymorphisms at the DNA level have been used in many investigations to determine the genetic diversity in plums. Previous molecular studies on plums using RAPD markers revealed a wide genetic polymorphism among different plum accessions ranging from 20 to 98% (Gregor et al, 1994;Ortiz et al, 1997;Bellini et al, 1998;Shimada et al, 1999;Lisek et al, 2007;Liu et al, 2007;Hend et al, 2009). This wide variation is normal because plums are very complex groups including diploid, tetraploid and hexaploid species and because flower biology also differs in different plum groups (Ercisli, 2004).…”

Section: Discussionmentioning

confidence: 99%

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Ertürk¹,

Erċışlı²,

Maghradze³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.

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Section: Discussionmentioning

confidence: 99%

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Ertürk¹,

Erċışlı²,

Maghradze³

et al. 2009

Genet. Mol. Res.

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“…Biochemical and molecular characterization of European and Japanese plums have been reported using isozymes (Byrne and Littleton, 1988) and random amplified polymorphic DNA (RAPD) analysis (Bellini et al, 1998;Gregor et al, 1994;Oritz et al, 1997;Shimada et al, 1999). These markers provide advantages over morphological observations, but still suffer from important problems of reproducibility and insufficient quantity of information per analyses.…”

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confidence: 99%

Phenetic Characterization of Plum Cultivars by High Multiplex Ratio Markers: Amplified Fragment Length Polymorphisms and Inter-simple Sequence Repeats

Goulão¹,

Monte-Corvo²,

Oliveira³

2001

jashs

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Variability of commercial plum (Prunus L. sp.) cultivars is unknown since breeding often involves intercrossing hybrids with several species but has been based on a low number of parents. Molecular markers like amplified fragment length polymorphisms (AFLP) and inter-simple sequence repeats (ISSR), which sample multiple loci simultaneously, have become increasingly popular, and were used to characterize 24 diploid and four hexaploid cultivars of plum. Seven AFLP and six ISSR primers were used, and resulted in amplification of 379 and 270 products, respectively. Unweighted pair-group method with arithmetic averages (UPGMA) dendrograms, based on similarity coefficients, reflected a clear separation between diploid and hexaploid plums. Among diploid plums, two pairs of cultivars were relatively distinct from the rest, namely `Golden Japan' and `Methley' and `Ozark Premier' and `Songold'. Furthermore, several cultivars were grouped together both with AFLP and ISSR analysis: 1) `Ambra', `Red Beaut', and `Black Beaut', 2) `Black Diamond' and `Royal Diamond', 3) `June Rose', `Santa Rosa', and `Royal Red', and iv) `Freedom', `Larry Ann', and `Queen Rosa'. Although the phenetic classification obtained by the two methods were similar (r = 0.73, for the diploid group), ISSR had a higher reproducibility and percentage of polymorphisms (87.4% vs. 62.8%) than AFLP. Methodological aspects of both markers systems are discussed. Results obtained suggest that the AFLP and ISSR approaches are valuable tools for identification of specific genotypes and analysis of phenetic relationships in plum.

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“…Polymorphisms at the DNA level have been used in several studies to examine genetic diversity in plums. Previous molecular studies on plums using RAPD markers revealed wide genetic polymorphism among accessions (Gregor et al,1994;Ortiz et al, 1997;Bellini et al, 1998;Shimada et al, 1999;Lisek et al, 2007;Liu et al, 2007;Ben Tamarzizt et al, 2009), which was explained by the floral biology and different ploidy levels. As demonstrated by Ortiz et al (1997), use of the arbitrary primers, AD10 and AD17, yields polymorphic amplification products that are specific to the diploid or hexaploid species.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, since tree fruit cultivars are maintained by vegetative propagation, accurate identification of vegetative material is crucial for nurserymen and growers, and is needed for plant breeder's rights (Goulao et al, 2001). Therefore, molecular markers, such as restriction fragment length polymorphism (RFLP) (Quarta et al, 1996), random amplified polymorphic DNA (RAPD) (Gregor et al, 1994;Warburton and Bliss, 1996;Ortiz et al, 1997;Bellini et al, 1998;Shimada et al, 1998;Casas et al, 1999;Lisek et al, 2007;Li et al, 2007;Ben Tamarzizt et al, 2009), inter simple sequence repeat (ISSR) (Yilmaz et al, 2009), simple sequence reapet SSR (Mnejja et al, 2004;Baraének et al,2006;Bouhadida et al, 2009), and amplified fragment length polymorphism (AFLP) (Ilgin et al, 2009), and sequences of non-coding region of chloroplast DNA (Ben Mustapha et al, 2013) have been tested. Knowledge of chromosome number and ploidy level is important, especially in plant families and genera where hybridization between species with different chromosome number or ploidy level occurs frequently.…”

Section: Introductionmentioning

confidence: 99%

DNA variation and polymorphism in Tunisian plum species (Prunus spp): contribution of flow cytometry and molecular markers

et al. 2015

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ABSTRACT. Plums (Prunus spp) are among the most important stone fruit crops in the world. European (Prunus domestica) and Japanese (Prunus salicina) plums are characterized by different levels of ploidy. Because genetic variability is the prerequisite for any plant-breeding program, we aimed to establish the taxonomic status of Tunisian plums and study their genetic variability. The nuclear DNA content of 45 wild and cultivated Tunisian plums was determined by flow cytometry. Two arbitrary primers (AD10, AD17) were used to elaborate SCAR markers useful to identify plum species. Three wild trees, Zenou 1, Zenou 6, and Zenou 3, which had 2C nuclear DNA contents of 1.99, 2.05, and 2.13 pg, were shown to be hexaploid (2n = 6x = 48), whereas the others were diploid (2n = 2x = 16). These results suggest that the three hexaploid wild plums 18035 DNA variation in Tunisian plum species ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18034-18046 (2015) belong to Prunus insititia, and the others belong to Prunus salicina. No SCAR markers were revealed using the AD10 and AD17 RAPD primers in relation to the ploidy of plums. We note also that AD17 primer appears to be the most informative concerning the genetic diversity. Morphological and pomological traits revealed similarity between introduced and Tunisian plum cultivars. Despite the significant morphological differences found, all the cultivars studied belong to P. salicina. The information obtained in this analysis provided on local plum genetic resources will be helpful to establish a core collection, to evaluate genetic diversity, and to initiate an improvement and selection program.

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Genetic Relationships in Japanese Plum Cultivars by Molecular Markers

Cited by 7 publications

References 0 publications

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Phenetic Characterization of Plum Cultivars by High Multiplex Ratio Markers: Amplified Fragment Length Polymorphisms and Inter-simple Sequence Repeats

DNA variation and polymorphism in Tunisian plum species (Prunus spp): contribution of flow cytometry and molecular markers

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