An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Ertürk, Yakup Erdal; Erċışlı, Sezai; Maghradze, D.; Orhan, Emine; Ağar, Güleray

doi:10.4238/vol8-4gmr641

Cited by 14 publications

(7 citation statements)

References 22 publications

(26 reference statements)

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“…Therefore, RAPD fingerprinting confirmed the morphological analyses results and also provided certain molecular markers that might be associated with certain commercial characteristics. In agreement with previous studies reported by Erturk et al (2009) on the characterization of peach cultivars by using RAPD markers, the results of the present study will allow for future studies on the appropriate use of these cultivars in breeding programs, proper biodiversity assessment and better conservation of germplasm resources.…”

Section: Discussionsupporting

confidence: 91%

Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR

Nagaty¹

2011

American Journal of Applied Sciences

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Problem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to amplify DNA from each cultivar by using Randomly Amplified Polymorphic DNA (RAPD) technology. RAPD profiles (i.e., cultivars) and RAPD amplicons (i.e., markers) were biclustered by using appropriate software and the RAPD biclusters were viewed as a heatmap in order to improve the display of RAPD markers. Results: The different RAPD primers produced a total of 114 fragments (amplicons), of which 63 were polymorphic among the studied cultivars. The RAPD fingerprinting results confirmed the data obtained from the morphological analyses and allowed for estimating the genetic relatedness among the studied peach cultivars. Moreover, the data indicated that certain molecular markers might be associated with certain commercial characteristics. Conclusion/Recommendations: Future studies on the association(s) of the selected molecular markers with fruiting characteristics should allow for new gene discovery, molecular breeding, proper biodiversity assessment and better conservation of germplasm resources

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Section: Discussionsupporting

confidence: 91%

Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR

Nagaty¹

2011

American Journal of Applied Sciences

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“…Prunus spinosa has a large distribution in the Euro-Siberian region. It occurs, in fact, in most of South-Central Europe, in NW Africa, reaching also Asia Minor, Caucasus and the Caspian Sea (Erturk et al 2009;Popescu and Caudullo 2016). Specifically, it is reported as native from the Atlantic shores of Portugal to the Mediterranean Coast of Israel (Carazo-Montijano and Fernández-López 2006;Dobignard and Chatelain 2013;Rhodes and Maxted 2016).…”

Section: Methodsmentioning

confidence: 99%

Distribution, ecological role and symbioses of selected shrubby species in the Mediterranean Basin: a review

Lombardo

Bancheva

Domina

et al. 2020

Plant Biosystems - An International Journal Dealing with all As

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Among the Mediterranean ecosystems, shrublands are a characteristic type of vegetation, widespread in different habitats. Owing to different factors such as the physiological, morphological, reproductive, phenological, and regenerative properties, as well as the inter-intraspecific interactions, each shrubby species represents an important element within the plant community and plays a specific ecological role. In this review, attention was focused on the ecological functions and type of plant-microsymbiont interactions in respect of selected shrubby species within the Mediterranean Basin: Amelanchier ovalis, Astragalus nebrodensis, Crataegus laciniata, Lycium intricatum, Prunus spinosa, and Viburnum tinus.

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“…Morphological study has revealed wide genetic polymorphism among plum accessions, because plums are a very complex group, which includes diploid, tetraploid, and hexaploid species, and because floral biology differs among plum groups (Erturk et al, 2009). The distribution of cultivars occurs independently from their geographic origin.…”

Section: Comparison Between Phenotypic Analysis and Flow Cytometrymentioning

confidence: 99%

DNA variation and polymorphism in Tunisian plum species (Prunus spp): contribution of flow cytometry and molecular markers

et al. 2015

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ABSTRACT. Plums (Prunus spp) are among the most important stone fruit crops in the world. European (Prunus domestica) and Japanese (Prunus salicina) plums are characterized by different levels of ploidy. Because genetic variability is the prerequisite for any plant-breeding program, we aimed to establish the taxonomic status of Tunisian plums and study their genetic variability. The nuclear DNA content of 45 wild and cultivated Tunisian plums was determined by flow cytometry. Two arbitrary primers (AD10, AD17) were used to elaborate SCAR markers useful to identify plum species. Three wild trees, Zenou 1, Zenou 6, and Zenou 3, which had 2C nuclear DNA contents of 1.99, 2.05, and 2.13 pg, were shown to be hexaploid (2n = 6x = 48), whereas the others were diploid (2n = 2x = 16). These results suggest that the three hexaploid wild plums 18035 DNA variation in Tunisian plum species ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18034-18046 (2015) belong to Prunus insititia, and the others belong to Prunus salicina. No SCAR markers were revealed using the AD10 and AD17 RAPD primers in relation to the ploidy of plums. We note also that AD17 primer appears to be the most informative concerning the genetic diversity. Morphological and pomological traits revealed similarity between introduced and Tunisian plum cultivars. Despite the significant morphological differences found, all the cultivars studied belong to P. salicina. The information obtained in this analysis provided on local plum genetic resources will be helpful to establish a core collection, to evaluate genetic diversity, and to initiate an improvement and selection program.

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An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Cited by 14 publications

References 22 publications

Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR

Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR

Distribution, ecological role and symbioses of selected shrubby species in the Mediterranean Basin: a review

DNA variation and polymorphism in Tunisian plum species (Prunus spp): contribution of flow cytometry and molecular markers

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