Genetic divergence landrace of langsat (Lansium parasiticum) from Siberut Island based on ITS and MatK markers

Huda, Miftahul; Syamsuardi,; Nurainas,; Murni, Pinta; Rayfiqa, Maulidah

doi:10.18805/a-398

Cited by 3 publications

(5 citation statements)

References 16 publications

(14 reference statements)

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“…macroura. The results of previous studies also revealed the morphological differences between cultivated variety from the different geographical regions (Moawed, 2015;Strbanovic et al, 2015) and between the wild infra-specific taxa (Tripathy et al, 2016;Huda et al, 2019) were detected.…”

Section: Resultsmentioning

confidence: 76%

“…Furthermore, molecular evidence is a significant character to support the stable identification of a taxon and classification of the taxonomic group. The haplotype is a set of DNA that can be used to identify differentiation between accessions (Huda et al, 2019) or infraspecific and species. In this study, the haplotype analysis was carried out on eleven sequences that consisting of seven sequences obtained from this study and four other sequences of DNA Clarification of Sumatran Mulberry (Morus macroura var.…”

Section: Resultsmentioning

confidence: 99%

“…This marker has proved as the best potential marker to clarified more than 6600 samples of the plants (Chen et al, 2010;Saddhe and Kumar, 2018). This marker also used to analyze the infraspecific tax, landrace and a unique population of the tropical plant (Huda et al, 2019) and authentication of medicinal plants (Hao et al, 2010;Techen et al, 2014;Kim et al, 2016;). It is also used to identify the material resources from mixed traditional medicine or adulterants (Zhou et al, 2014;Doh et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Clarification of Sumatran Mulberry (Morus macroura var. macroura, Moraceae) from West Sumatra, Indonesia using Nucleus Ribosomal ITS (Internal Transcribed Spacer) Gene

Nuratika

Aseny

Syamsuardi

et al. 2020

IJARe

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Background: Morus macroura Miq. is a potential tree species belonging to the Mulberry group, and consider as flora identity of West Sumatra Province with local name is Andalas. This germplasm tropical tree species utilize widely by local West Sumatran people for various purposes such as wood material to construct the traditional house of ethnic Minangkabau and medicinal substance for multiple diseases. However, the population numbers of this species were decreased and rare due to overexploitation without effort for planting. Based on this reason, it is imperative to conserve and utilize sustainable Mulberry. There is a highlight confusion to clarify that M. macroura from Sumatra population similar to Himalayan Mulberry. For this reason, it is essential to define the crucial local M. macroura from Sumatra (Sumatran Mulberry) for its conservation and sustainable utilization. Method: In this study, the leaves from seven individuals of M. macroura collected from West Sumatra were used for DNA extraction and processed to the next step of the analysis. Four individuals of Himalayan Mulberry and both of two out-group, White Mulberry (M. alba), and Black Mulberry (M. nigra) were used in this study. Polymerase chain reaction (PCR) amplification was performed using two ITS markers (ITS5-F and ITS4-R) with an annealing temperature of 55oC. The PCR products were purified and sequenced by Macrogen USA DNA Sequencing. The phyllogenetic relationship between seven accessions and two of out-group (M. alba and M. nigra) were analyzed using the MEGA6 program. Result: The result indicated that all of the accessions M. macroura from Sumatra were grouped into the same cluster and separated to the Himalayan Mulberry group. The differentiation between Sumatran Mulberry and other Mulberry reflected in morphological and haplotype differentiation indicated that the Sumatran Mulberry as the different group and suggested as a variety with scientific name is M. macroura Miq. var. macroura. This clarification is useful for conservation and sustainable utilization of this variety, a specific Mulberry from Sumatra.

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clarification of Sumatran Mulberry (Morus macroura var. macroura, Moraceae) from West Sumatra, Indonesia using Nucleus Ribosomal ITS (Internal Transcribed Spacer) Gene

Nuratika

Aseny

Syamsuardi

et al. 2020

IJARe

Self Cite

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“…Molecular characterization and identification play a vital role in distinguishing plants at the level of individuals, populations, races, and plant species (Huda et al, 2019;Nuratika and Aseny, 2020). However, various molecular techniques applied to distinguish between plant groups were generally DNA sequencing or RAPD and SRAP techniques.…”

Section: Discussionmentioning

confidence: 99%

Identification of Pinus Merkusii Landrace Belonging to Kerinci - West Sumatra, Indonesia, Using Sequence-Related Amplified Polymorphism (Srap) Technique

Rinaldi¹,

Nurainas²,

Syamsuardi³

2023

SABRAOJBG

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Pinus merkusii is a potential wood that naturally grows in Central Sumatra (Kerinci) and North Sumatra (Aceh and Tapanuli), Indonesia. The Kerinci landrace has a different morphology from the other two Sumatran Pinus landraces, namely, Aceh and Tapanuli, but its placement is at the same taxonomic level. Hence, additional characters need evaluation to validate taxa on P. merkusii landrace belonging to Kerinci. This study aims to identify differences in genetic characters between Kerinci and Aceh (Rao and TAHURA [Taman Hutan Raya]) landraces using SRAP for long-term use and future conservation. DNA extraction ran from the leaves of 24 individual trees belonging to three populations of P. merkusii, i.e., one naturally occurring population in Kerinci and the two cultivated populaces found in Rao and TAHURA, Indonesia. Using seven SRAP combination primers, the analysis revealed an immense variety of alleles (bands). Overall, the amplification produced 62 bands, with nine, on average, per primary pair. However, most bands were polymorphic (91.79%), and only 0.57% were monomorphic. With 45% informative bands, the Kerinci landrace has the highest band variation. A combination of primer C (Me1 + Em3) at 900 bp and primer I (Me3 + Em1) at 1050 bp band size indicated specific bands that served as molecular characteristics. A crucial molecular characteristic distinguishes the landraces of P. merkusii found in Kerinci and other populations in Rao and TAHURA, Indonesia. The study revealed superior genetic variation among the P. merkusii populations evaluated.

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“…All the five variants of langsat were locally called Lansat Padang, Attelu turugougou, Siamung, Elak mata, and Setcet. Huda et al state that there are five local germplasms of Langsat from Siberut and based on the phylogenetic trees showed that the accessions of langsat from Siberut Island were separated from accessions of Sumatran mainland [10]. While for durian's germplasm, Mentawai durian (Durio lowianus), locally called Toktuk, was different from durians found on the mainland Sumatra (Durio zibethinus).…”

Section: Plant Species and Their Uses In Various Land Types In The Traditional Plantation In Mentawaimentioning

confidence: 99%

Ethnobotanical Aspects of Mentawai Traditional Agricultural System (Pumonean) and Its Implications for the Conservation of Local Germplasm in Siberut, Mentawai, Indonesia

Nurainas¹,

Syukma²,

Chairul³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Mentawai people have a unique agricultural tradition. The traditional agricultural system (mone) is adaptive, using the plants that are suitable for the landscape. Land clearing is carefully done without destroying the entire forest. We conducted ethnobotany studies to explore the composition and the structure of the plants used in the traditional plantation (Pumonean) as well as their values for the Mentawai people in the Siberut island. We surveyed the agricultural system employed by Mentawai people and conducted vegetation assessment in the study sites. The data on the plant species and land usages were gathered from interviews with key informants in the areas. We analyzed the data using Local User's Value Index (LUVI). The plants were collected to aid with species identification and were stored at the Andalas University Herbarium (ANDA), Padang. Throughout the study, we recorded 64 species in 27 families. Euphorbiaceae was the family with the highest species diversity. Sagu (Metroxylon sago) was the species with the highest LUVI. We categorized the land usage for the traditional plantation into four categories, consisting of wetland, the edge of wetland, mixed plantations (mone), and forests (leleu). The mixed plantation was the category with the highest LUVI. Beside the traditional agricultural system, the Mentawai people also developed a more modern agricultural system. For the traditional system, Durio zibethinus was the species with the highest importance indices, whereas for the more modern system Theobroma cacao was the species with the highest importance indices.

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Genetic divergence landrace of langsat (Lansium parasiticum) from Siberut Island based on ITS and MatK markers

Cited by 3 publications

References 16 publications

Clarification of Sumatran Mulberry (Morus macroura var. macroura, Moraceae) from West Sumatra, Indonesia using Nucleus Ribosomal ITS (Internal Transcribed Spacer) Gene

Clarification of Sumatran Mulberry (Morus macroura var. macroura, Moraceae) from West Sumatra, Indonesia using Nucleus Ribosomal ITS (Internal Transcribed Spacer) Gene

Identification of Pinus Merkusii Landrace Belonging to Kerinci - West Sumatra, Indonesia, Using Sequence-Related Amplified Polymorphism (Srap) Technique

Ethnobotanical Aspects of Mentawai Traditional Agricultural System (Pumonean) and Its Implications for the Conservation of Local Germplasm in Siberut, Mentawai, Indonesia

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