Genetic characterization of Zanskari breed of horse

Behl, Rahul; Behl, Jyotsna; Gupta, Neelam; Gupta, S. C.; Ahlawat, Sonika; Ragnekar, Mahesh; Ahmed, Zaheer

doi:10.1007/bf02935331

Cited by 10 publications

(12 citation statements)

References 26 publications

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“…Due to different allele frequencies, the effective number of alleles has a different probability of taking part in mating. In our study, the mean effective number of (Juras & Cothran, 2004;Behl et al, 2006;Shahsavarani & Rahimi-Mianji, 2010).…”

Section: Microsatellite Analysismentioning

confidence: 54%

“…In our study, the mean effective number of alleles in the Hucul horse population was 4.401, ranging from 2.116 ( HMS1 ) to 6.330 ( VHL20 ). This value differs from that of the Lithuanian heavy draught horse (1.557–5.969), Indian Zanskari horse (3.07–7.01) or Caspian horse (3.49–8.49) (Juras & Cothran, ; Behl et al ., ; Shahsavarani & Rahimi‐Mianji, ).…”

Section: Discussionmentioning

confidence: 99%

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Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Kusza

Priskin

Ivanković

et al. 2013

Biol J Linn Soc Lond

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The aim of this work was to gather information about the origin and genetic characterization of the Central European Hucul horse based on 71 horses using 17 microsatellites and the D‐loop region of mtDNA. Their genetic relationship to the Polish Konik (N = 7), German (N = 4) and Hungarian wild Przewalski horses (N = 4) and 200 horse sequences from GenBank was also analysed. Both microsatellite and mtDNA analysis showed a high genetic variation in the Hucul. A total of 130 alleles were detected, the mean number of observed alleles per microsatellite was 7.647, and the number of effective alleles was 4.401. The average observed and expected heterozygosity were 0.706 and 0.747, respectively. The high heterozygosity values and Wright's fixation index (FIS) (−0.128) indicated a low level of inbreeding, low or no selection pressure, and large number of alleles. mtDNA analysis revealed 18 haplotypes for the Hucul population with a total of 23 variable sites. Haplotype and nucleotide diversities were 0.935 ± 0.011 and 0.022 ± 0.012, respectively. Neutrality tests (Tajima's D and Fu's Fs) were non‐significant, and mismatch distribution was ragged, indicating that the Hucul population is in genetic equilibrium. The most frequent mtDNA D‐loop region belonged to haplogroup A (48%), which was also present in Przewalski Wild horse samples, while Polish Konik samples belonged to three haplotypes and C, F, and G haplogroups. Large and significant pairwise ΦST values along with a small number of common haplotypes indicated a low level of gene flow and lack of genetic structure among the three studied breeds (Hucul, Konik, and Przewalski Wild horse). The present work contributes to our knowledge of the genetic diversity of the Hucul horse and helps to define its genetic conservation. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2013, 109, 54–65.

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Section: Microsatellite Analysismentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Kusza

Priskin

Ivanković

et al. 2013

Biol J Linn Soc Lond

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“…The polymorphism at different loci as reflected by means of the number of alleles was sufficiently high, while these values reported in earlier studies in Spiti and Zanskari pony breeds were quite low (Behl et al, 2006a(Behl et al, , 2006b) which could probably be due to the difference in microsatellites selected, methodology used for genotyping (manual silver staining versus automatic sequencer) as well as sampling from a small/limited population. Higher number of observed alleles than expected at 15 different loci indicated the presence of high genetic diversity at these loci.…”

Section: Discussionmentioning

confidence: 67%

“…Among the recent DNA based technologies, microsatellites are considered by many as the most suitable marker system for evaluating genetic diversity owing to their abundance in the mammalian genome, high level of polymorphism, co-dominant inheritance and amenability for automation (Takezaki and Nei, 1996). Some limited studies on phenotypic and genetic characterization of this breed have been carried out (Gupta et al, 2010;Behl et al, 2006aBehl et al, , 2006b. The present study includes molecular characterization, based on a battery of specific microsatellites useful for such studies in horses to detect historical bottleneck if any in Zanskari population.…”

Section: Introductionmentioning

confidence: 98%

Microsatellite markers based genetic diversity and bottleneck studies in Zanskari pony

Gupta

Chauhan

Bhardwaj

et al. 2012

Gene

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“…Published reports of polymorphisms with microsatellites in other equine breeds range from 0.610 for the Zanskari horse breed (Behl et al, 2006), 0.663 in Lipizzaners (Achmann et al, 2004), 0.647 for Spanish Trotters (Azor et al, 2007), 0.745 for Garranos, and 0.677 for Lusitanos (Luís et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

Giacomoni¹,

Fernández²,

Freitas³

2008

Genet. Mol. Res.

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AbSTRAcT. The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity exclusion probability was 99.3%. The inbreeding coefficient (F IS ) was low for the three populations: Ipiranga (F IS = 0.147), Nova Esperança (F IS = 0.094) and Promissão (F IS = 0.108). Genetic differentiation among all three populations was low (F ST = 0.008 to 0.064). Three methods were used to test for a recent bottleneck effect. The graphical method and the Wilcoxon test using the stepwise mutation model showed no bottleneck pattern for any of the populations. The test by two-phase mutation model showed genetic signatures of bottleneck for Ipiranga and Promissão. When we consider standard deviation value for Nova Esperança, the M-statistic detected a bottleneck pattern, but this result could be explained by a sample size effect. Therefore, there is no immediate cause for concern regarding loss of variation within the breed.

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Genetic characterization of Zanskari breed of horse

Cited by 10 publications

References 26 publications

Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Microsatellite markers based genetic diversity and bottleneck studies in Zanskari pony

Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

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