This paper presents the results of a health monitoring study, carried out during the destructive load testing of a prototype reinforced concrete (RC) bridge. The bridge was made up of cement-concrete reinforced with steel rods, and represented a popular class of road bridges in which regular health monitoring is a very important issue during the service life. The bridge was instrumented with piezoceramic transducer (PZT) patches, which were electrically excited at high frequencies, of the order of kHz, and the real part of admittance (conductance) was extracted as a function of the exciting frequency. The patches were scanned for the acquisition of this signature at various stages during the loading process. The signatures of the patches located in the vicinity of the damage were found to have undergone drastic changes, while those farther away were less affected. Damage was quantified in non-parametric terms using the root mean square of the deviation in signatures with respect to the baseline signature of the healthy state. This non-parametric index was found to correlate well with the damage progression in the structure.

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The rapid increase of toxic dye wastewater generated from various industries remains a severe public health problem and of prime environmental protection concern. Therefore, it has imposed a major challenge...

Using a pharmacological inhibitor of Hsp90 in cultured malarial parasite, we have previously implicated Plasmodium falciparum Hsp90 (PfHsp90) as a drug target against malaria. In this study, we have biochemically characterized PfHsp90 in terms of its ATPase activity and interaction with its inhibitor geldanamycin (GA) and evaluated its potential as a drug target in a preclinical mouse model of malaria. In addition, we have explored the potential of Hsp90 inhibitors as drugs for the treatment of Trypanosoma infection in animals. Our studies with full-length PfHsp90 showed it to have the highest ATPase activity of all known Hsp90s; its ATPase activity was 6 times higher than that of human Hsp90. Also, GA brought about more robust inhibition of PfHsp90 ATPase activity as compared with human Hsp90. Mass spectrometric analysis of PfHsp90 expressed in P. falciparum identified a site of acetylation that overlapped with Aha1 and p23 binding domain, suggesting its role in modulating Hsp90 multichaperone complex assembly. Indeed, treatment of P. falciparum cultures with a histone deacetylase inhibitor resulted in a partial dissociation of PfHsp90 complex. Furthermore, we found a well known, semisynthetic Hsp90 inhibitor, namely 17-(allylamino)-17-demethoxygeldanamycin, to be effective in attenuating parasite growth and prolonging survival in a mouse model of malaria. We also characterized GA binding to Hsp90 from another protozoan parasite, namely Trypanosoma evansi. We found 17-(allylamino)-17-demethoxygeldanamycin to potently inhibit T. evansi growth in a mouse model of trypanosomiasis. In all, our biochemical characterization, drug interaction, and animal studies supported Hsp90 as a drug target and its inhibitor as a potential drug against protozoan diseases.

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