Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

Giacomoni, Elise Hofheinz; Fernández, Guillermina; Freitas, Thales Renato Ochotorena de

doi:10.4238/vol7-1gmr367

Cited by 25 publications

(28 citation statements)

References 30 publications

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“…Intermediate values, similar to those reported here for the MM breed, were also described for Lippizan (0.663), Sable Island (0.626), and Anglo-Arab (0.66) horses (Kelly et al, 2002;Tozaki et al, 2003;Achmann et al, 2004;Luís et al, 2002Luís et al, , 2005Plante et al, 2007). Recently, a relatively high mean PIC (0.700) was reported for the Pantaneiro horse, an endangered Brazilian feral horse breed (Giacomoni et al, 2008 Table 2. Wright F-statistics.…”

Section: Discussionsupporting

confidence: 83%

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

DeAssis¹,

DeLaat²,

Peixoto³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. One hundred and fifteen unrelated Mangalarga Marchador horses were sampled from three geographically distinct regions of Minas Gerais State, Brazil (South, Southeast, and Northeast) and tested for 10 microsatellite loci. Genetic diversity and population structure parameters were estimated with ARLEQUIN 3.0, CERVUS 2.0, POPGENE 1.31, GENEPOP on the web, STRUCTURE 2.0, and SPAGEDI 1.2 software packages. Under Hardy-Weinberg assumptions, seven markers were at equilibrium (LEX014, LEX017, LEX019, SGCV23, TKY321, VHL20, and VIASH39), while two (ASB3 and LEX031) presented significant homozygote excess. Seventy-four alleles were identified in these nine markers, with a mean of 8.22 alleles. Mean heterozygosity was 0.637 and polymorphism information content was 0.662. Markers ASB3, LEX019, SGCV23, TKY321, and VHL20 were highly informative (PIC >0.7) and may be useful for eventual expansion of parentage test panels. The F ST value (0.0562) indicated relatively little geographical structure. However, based on a Bayesian-based cluster analysis under a three-cluster model, 94% of the 115 individuals were correctly assigned to the subpopulations from where they were sampled. Mean pairwise f was relatively high (0.11), and in spite of the efforts towards non-consanguineous sampling, 1% of the pairs of individuals shared over 50% of the alleles. These results strongly suggest that the population is genetically structured. Under a conservation genetics approach, two strategies are recommended: avoidance of crosses between highly endogamic individuals and stimulation of crosses between individuals from those regions for which low genetic flow was identified.

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Section: Discussionsupporting

confidence: 83%

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

DeAssis¹,

DeLaat²,

Peixoto³

et al. 2009

Genet. Mol. Res.

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“…When we look at native horses all over the world, most of them are on the verge of extinction [13]. The Kiso horse experienced rapid loss of the population causing a bottleneck, which we confirmed here, and is categorized as one of such endangered breeds [27,28].…”

Section: Discussionsupporting

confidence: 56%

“…Therefore, some efforts have made by breeder associations or governments to conserve native horses based on scientific ways [4,13,27]. Similarly, we started to work with the Kiso Horse Conservation Association to create a management program for conservation, and studied the genetic diversity of the Kiso horse in this study.…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of the Endangered Kiso Horse Using 31 Microsatellite DNAs

et al. 2012

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ABSTRACT. In order to contribute to conservation of the endangered Kiso horse, we clarified their genetic information using 31 microsatellite DNAs, and genotyped 125 horses, 83% of the existing breed. First, we clarified the current status of the horses. The horses were confirmed to have experienced rapid loss of population causing a bottleneck, and their effective population size was much smaller than their census size. Moreover, the number of alleles (6.3), observed heterozygosity (0.674), and expected heterozygosity (0.662) were in the same range as other endangered horses all over the world. Therefore, although their inbreeding level was not so severe (F is : -0.017), the Kiso horse is surely one of the endangered. Second, we obtained genetic information of individuals. This information allowed us to understand the genetic distance of individuals, and might help in development of a reproductive strategy concerning the genetic distance between the mating pairs. Moreover, there appeared to be 4 subpopulations of Kiso horse, and this result was in good agreement with their historical background. Third, we confirmed that the parentage test for identification using the 31 microsatellite DNAs was highly reliable (probability of exclusion: 0.999999993). This identification increases the reliability of stud certification, and is also helpful for effective management. Understanding the genetic diversity within the population and the relationships among individuals is important to ensuring effective management for maintenance of genetic variation, and this study may help in conservation of the endangered Kiso horse.

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“…Thus, the high polymorphism found among Criollo horses in this study is striking, even more so when one compares the average number of alleles per locus with other national (Luis et al, 2007b) and foreign herds (Kelly et al, 2002;Luis et al, 2007b;Paredes et al, 2009), or even other breeds (Bowling et al, 1997;Jakabová, 2002;Solis et al, 2005;Lee and Cho, 2006;Kakoi et al, 2007;Luis et al, 2002Luis et al, , 2007aPlante et al, 2007;Giacomoni et al, 2008;Perez-Gutierrez et al, 2008;Thirstrup et al, 2008;Bomcke and Gengler, 2009;LeRoy et al, 2009). This particularity can be attributed to the short period since the closing of its stud book or the constant migration of animals (Thirstrup et al, 2008) solely allowed between countries affiliated with FICCC.…”

Section: Discussionmentioning

confidence: 59%

“…Deviation from Hardy-Weinberg equilibrium and heterozygote deficit were already described in previous publications evaluating Criollo horses (Kelly et al, 2002;Luis et al, 2007b;Paredes et al, 2009), but never in all the reported loci shown by a single study. In several other horse breeds, small differences were also described (Jakabová, 2002;Solis et al, 2005;Lee and Cho, 2006;Kakoi et al, 2007;Luis et al, 2002Luis et al, , 2007aGiacomoni et al, 2008;Perez-Gutierrez et al, 2008;Thirstrup et al, 2008).…”

Section: Discussionmentioning

confidence: 95%

Genotyping in the Brazilian Criollo Horse Stud Book: resources and perspectives

Costa¹,

Bressel²,

Almeida³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The goal of this research was to evaluate the ability of the genotyping information available in the Brazilian Criollo Horse Stud Book to describe the genetic variability of the breed and the exclusion probability determined in comparative tests. Altogether, two softwares were used in the analyses of the available genotypes: Cervus 3.0.3 and Genepop 4.0. Eight microsatellite markers totaled 109 alleles, with an average of 13.6 ± 0.6 alleles per locus. Large differences between expected and observed heterozygosity were ubiquitous (0.821 ± 0.07 and 0.470 ± 0.17, respectively). Although the estimated null allele frequency caused initial concern (0.284 ± 0.199), it is likely that it was a reflection of the inbreeding coefficients found (0.432 ± 0.184). All loci showed significant deviation from Hardy-Weinberg equilibrium, with heterozygote deficit (P < 0.0001) and genotypic linkage disequilibrium with at least one marker. The high polymorphic information content (0.798 ± 0.088) could not warrant exclusion power for three loci (HMS7, HMS6 and HTG4) above 50% (0.491 ± 0.158). However, combined exclusion probability reached 99.61%, a level close to ideal. The results demonstrate the excellent performance of the markers assessed in describing the genetic status of the breed and suggest the considerable ability to establish parentage.

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Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers

Cited by 25 publications

References 30 publications

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

Genetic diversity and population structure in Brazilian Mangalarga Marchador horses

Genetic Characterization of the Endangered Kiso Horse Using 31 Microsatellite DNAs

Genotyping in the Brazilian Criollo Horse Stud Book: resources and perspectives

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