Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t)

Abstract

Genetic analysis and fine mapping of a resistance gene against brown planthopper (BPH) biotype 2 in rice was performed using two F(2) populations derived from two crosses between a resistant indica cultivar (cv.), AS20-1, and two susceptible japonica cvs., Aichi Asahi and Lijiangxintuanheigu. Insect resistance was evaluated using F(1) plants and the two F(2) populations. The results showed that a single recessive gene, tentatively designated as bph19(t), conditioned the resistance in AS20-1. A linkage analysis, mainly employing microsatellite markers, was carried out in the two F(2) populations through bulked segregant analysis and recessive class analysis (RCA), in combination with bioinformatics analysis (BIA). The resistance gene locus bph19(t) was finely mapped to a region of about 1.0 cM on the short arm of chromosome 3, flanked by markers RM6308 and RM3134, where one known marker RM1022, and four new markers, b1, b2, b3 and b4, developed in the present study were co-segregating with the locus. To physically map this locus, the bph19(t)-linked markers were landed on bacterial artificial chromosome or P1 artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. Sequence information of these clones was used to construct a physical map of the bph19(t) locus, in silico, by BIA. The bph19(t) locus was physically defined to an interval of about 60 kb. The detailed genetic and physical maps of the bph19(t) locus will facilitate marker-assisted gene pyramiding and cloning.

Current Status of Brown Planthopper (BPH) Resistance and Genetics

“…The Bph13 gene resides in the region flanked by RG100 and RG 191 region (Renganayaki et al 2002), while the bph19 gene was mapped in the region flanked by RM517 and RM218 (Chen et al 2006). The bph11 and Bph14 genes also were mapped in the nearby region on chromosome 3L and both of them have been identified in an introgression line of O. officinalis.…”

“…The Bph13(t) gene was reported to be resistant to the Indian biotype of BPH (biotype 4) and resistance was controlled by a single dominant gene. Chen et al (2006) fine-mapped the bph19(t) gene with flanking SSR markers (RM6308 and RM3134) on the short arm of chromosome 3 located at a distance of 1 cM. Sequence information of BAC clones was used to construct a physical map of bph19(t) gene and the locus was physically defined to an interval of about 60 kb.…”

Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

“…To date, a number of major BPH resistance genes have been identiWed in indica rice cultivars and the wild rice species O. australiensis, O. eichingeri, O. latifolia and O. oYcinalis (Khush et al 1985;Kabir and Khush 1988;Nemoto et al 1989;Ishii et al 1994;Hirabayashi and Ogawa 1995;Murata et al 1998;Kawaguchi et al 2001;Murai et al 2001;Murata et al 2001;Chen et al 2006). Quantitative trait loci (QTLs) that confer BPH resistance have also been found in certain indica cultivars, such as IR64 (Alam and Cohen 1998), Teqing , B5 (Ren et al 2004) and Col.5 Thailand (Sun et al 2007).…”

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

“…The development of a novel BPH resistant cultivar is thought to be the most effective manner to combat this pest. A number of major BPH-resistance genes have been identified from indica rice cultivars (Chen et al 2006, Kabir and Khush 1988, Khush 1979, Khush et al 1985, Nemoto et al 1989, Sun et al 2005. The development of near-isogenic lines (NILs) and pyramided lines (PYLs) carrying one or several resistance genes has been performed (Jairin et al 2009, Sharma et al 2004 and is a most effective manner to facilitate the use of the BPH-resistance genes or of quantitative trait loci (QTLs) for future rice improvement.…”