Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

Park, Dong-Soo; Song, Min-Young; Park, Soo-Kwon; Lee, Sang‐Kyu; Lee, Jonghee; Song, Song-Yi; Eun, Moo Young; Hahn, Tae‐Ryong; Sohn, Jae-Keun; Yi, Gihwan; Nam, Min‐Hee; Jeon, Jong‐Seong

doi:10.1007/s00438-008-0353-2

Cited by 18 publications

(13 citation statements)

References 71 publications

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“…The rice brown planthopper ( Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)) is one of the most destructive monophagous insect pest of rice, Oryza sativa , it draw nutrients from the phloem of rice plants and cause huge yield losses every year in rice grown throughout tropical, subtropical and temperate areas in Asia[ 1 , 2 ]. RNAi (targeted silencing of gene expression by injection of corresponding dsRNA) has become a major tool in functional analysis of genes in insects [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Ras-like family small GTPases genes in Nilaparvata lugens: Identification, phylogenetic analysis, gene expression and function in nymphal development

Wang

Wan

et al. 2017

PLoS ONE

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Twenty-nine cDNAs encoding Ras-like family small GTPases (RSGs) were cloned and sequenced from Nilaparvata lugens. Twenty-eight proteins are described here: 3 from Rho, 2 from Ras, 9 from Arf and 14 from Rabs. These RSGs from N.lugens have five conserved G-loop motifs and displayed a higher degree of sequence conservation with orthologues from insects. RT-qPCR analysis revealed NlRSGs expressed at all life stages and the highest expression was observed in hemolymph, gut or wing for most of NlRSGs. RNAi demonstrated that eighteen NlRSGs play a crucial role in nymphal development. Nymphs with silenced NlRSGs failed to molt, eclosion or development arrest. The qRT-PCR analysis verified the correlation between mortality and the down-regulation of the target genes. The expression level of nuclear receptors, Kr-h1, Hr3, FTZ-F1 and E93 involved in 20E and JH signal pathway was impacted in nymphs with silenced twelve NlRSGs individually. The expression of two halloween genes, Cyp314a1 and Cyp315a1 involved in ecdysone synthesis, decreased in nymphs with silenced NlSar1 or NlArf1. Cyp307a1 increased in nymphs with silenced NlArf6. In N.lugens with silenced NlSRβ, NlSar1 and NlRab2 at 9th day individually, 0.0% eclosion rate and almost 100.0% mortality was demonstrated. Further analysis showed NlSRβ could be served as a candidate target for dsRNA-based pesticides for N.lugens control.

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Section: Introductionmentioning

confidence: 99%

Ras-like family small GTPases genes in Nilaparvata lugens: Identification, phylogenetic analysis, gene expression and function in nymphal development

Wang

Wan

et al. 2017

PLoS ONE

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“…As reported in the literature, Bph13(t) was located on chromosome 2; Bph11, Bph13, Bph14, and Bph19 genes on chromosome 3; Bph12, Bph15, Bph17, and Bph20 genes on chromosome 4 (Rahman et al 2009); Bph, and bph4 genes on chromosome 6 (Kawaguchi et al 2001); Bph6 on chromosome 11 (Jena et al 2002); Bph1, bph2, Bph9, Bph10, Bph18, and Bph21 genes on chromosome 12 (Jena et al 2006;Sharma et al 2004). Park et al (2008) used representational difference analysis (RDA) to identify OsBphi252 that is tightly linked to BPH resistance on chromosome 12. It is clear that there are inconsistencies in assigning gene number and locating its position on the chromosome (Table 3).…”

Section: Genetics Of Bph Resistancementioning

confidence: 99%

Current Status of Brown Planthopper (BPH) Resistance and Genetics

Jena¹,

Kim²

2010

Rice

183

119

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Among the planthoppers of rice, the brown planthopper (BPH) is a major threat to rice production and causes significant yield loss annually. Host-plant resistance is an important strategy to reduce the damage caused by BPH and increase rice productivity. Twenty-one major genes for BPH resistance have been identified by using standard evaluation methods developed at the International Rice Research Institute (IRRI) to distinguish resistance or susceptibility of rice genotypes to BPH biotypes/populations. These genes are from diverse genetic resources such as land race cultivars and wild species of Oryza. Of the 21 resistance genes, 18 genes have been localized on specific region of six rice chromosomes using molecular genetic analysis and genomics tools. Some of these resistance genes are clustered together such as Bph1, bph2, Bph9, Bph10, Bph18, and Bph21 on the long arm of chromosome 12; Bph12, Bph15, Bph17 and Bph20 on the short arm of chromosome 4; bph11 and Bph14 on the long arm of chromosome 3 and Bph13(t) and bph19 on the short arm of chromosome 3. Six genes (Bph11, bph11, Bph12, bph12, Bph13 and Bph13) originated from wild Oryza species have either duplicate chromosome locations or wrong nomenclature. The discrepancy should be confirmed by allelism tests. Besides identification of major resistance genes, some quantitative trait loci (QTLs) associated with BPH resistance have also been identified on eight chromosomes. Most of the rice cultivars developed at IRRI possess one or two of the major resistance genes and the variety IR64 has many QTLs and confers strong resistance to BPH. More BPH resistance genes need to be identified from the wealth of gene pool available in the wild species of Oryza. Two BPH resistance genes (Bph14 and Bph18) have been cloned, and a snow drop lectin gene (GNA) has been identified and used in the development of BPH-resistant transgenic plants. Efficient introgression of resistance genes (Bph1, bph2, Bph3, Bph14, Bph15, Bph18, Bph20, and Bph21) into elite rice cultivars by marker-assisted selection together with strategic deployment of these genes can be an important approach to develop stable resistance to BPH and sustain rice production in the tropical and temperate rice growing regions.

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“…Even though, there are many constraints in rice production, insects' pests remain a constant problem in all the rice growing regions (Manikandan Narayanasamy et al, 2014). Brown planthopper (BPH), Nilaparvata lugens (Stal) (Hemiptera: Delphacidae) is a typical piercing sucking insect pest of rice (Oryza sativa L.; Poaceae), which feeds on phloem sap and thus affects the growth of rice and results in "hopper burn" in rice fields (Park et al, 2008). In addition to direct damage, BPH also transmits viruses, such as the ragged stunt virus and grassy stunt virus, and associated diseases to rice plants (Jena et al, 2006 (Kalode, 1974;Kulshrestha et al, 1974).…”

Section: Introductionmentioning

confidence: 99%

Anatomical Studies of Wild Rice Accession for Evaluation of Resistance against Brown Plant Hopper

Gajbhiye¹,

Kumar²,

Pandey³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis

Cited by 18 publications

References 71 publications

Ras-like family small GTPases genes in Nilaparvata lugens: Identification, phylogenetic analysis, gene expression and function in nymphal development

Ras-like family small GTPases genes in Nilaparvata lugens: Identification, phylogenetic analysis, gene expression and function in nymphal development

Current Status of Brown Planthopper (BPH) Resistance and Genetics

Anatomical Studies of Wild Rice Accession for Evaluation of Resistance against Brown Plant Hopper

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