Current Status of Brown Planthopper (BPH) Resistance and Genetics

Abstract

Among the planthoppers of rice, the brown planthopper (BPH) is a major threat to rice production and causes significant yield loss annually. Host-plant resistance is an important strategy to reduce the damage caused by BPH and increase rice productivity. Twenty-one major genes for BPH resistance have been identified by using standard evaluation methods developed at the International Rice Research Institute (IRRI) to distinguish resistance or susceptibility of rice genotypes to BPH biotypes/populations. These genes are from diverse genetic resources such as land race cultivars and wild species of Oryza. Of the 21 resistance genes, 18 genes have been localized on specific region of six rice chromosomes using molecular genetic analysis and genomics tools. Some of these resistance genes are clustered together such as Bph1, bph2, Bph9, Bph10, Bph18, and Bph21 on the long arm of chromosome 12; Bph12, Bph15, Bph17 and Bph20 on the short arm of chromosome 4; bph11 and Bph14 on the long arm of chromosome 3 and Bph13(t) and bph19 on the short arm of chromosome 3. Six genes (Bph11, bph11, Bph12, bph12, Bph13 and Bph13) originated from wild Oryza species have either duplicate chromosome locations or wrong nomenclature. The discrepancy should be confirmed by allelism tests. Besides identification of major resistance genes, some quantitative trait loci (QTLs) associated with BPH resistance have also been identified on eight chromosomes. Most of the rice cultivars developed at IRRI possess one or two of the major resistance genes and the variety IR64 has many QTLs and confers strong resistance to BPH. More BPH resistance genes need to be identified from the wealth of gene pool available in the wild species of Oryza. Two BPH resistance genes (Bph14 and Bph18) have been cloned, and a snow drop lectin gene (GNA) has been identified and used in the development of BPH-resistant transgenic plants. Efficient introgression of resistance genes (Bph1, bph2, Bph3, Bph14, Bph15, Bph18, Bph20, and Bph21) into elite rice cultivars by marker-assisted selection together with strategic deployment of these genes can be an important approach to develop stable resistance to BPH and sustain rice production in the tropical and temperate rice growing regions.

Allelic diversity in an NLR geneBPH9enables rice to combat planthopper variation

“…Interestingly, most of these genes are mapped on several chromosome regions in clusters (12)(13)(14). The cluster on the long arm of chromosome 12 (12L) is the largest one that harbors eight BPHresistance genes, including the most widely used BPH1 and BPH2 (12,14).…”

“…In addition to BPH9, seven BPH-resistance genes (BPH1, BPH2, BPH7, BPH10, BPH18, BPH21, and BPH26) have been reported on chromosome 12L (12,14,(26)(27)(28)(29)(30). Among them, BPH26 has been cloned, and BPH2 is identical to BPH26 (ref.…”

Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants

“…It directly damages the plant by sucking phloem sap, causing hopper burn, and transmitting viral diseases. In 2005 and 2008, China reported a combined yield loss of 2.7 million tons of rice due to direct damage by N. lugens [13]. The impact of this pest was attributed to its ability to adapt ecologically and physiologically to environmental factors, as well as its direct and indirect impacts on rice yield [14].…”

Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

“…Since 1980, more than 20 BPH resistance genes affecting rice cultivars have been genetically mapped; only 2 of them (bph14 and bph18) have been cloned and characterized (Jena and Kim, 2010;Zhou et al, 2013). Validation and practical application of those discoveries in the form of MAS in rice breeding programs shows that the target genes can be identified more efficiently in segregating populations at any plant growth stage with the use of tightly linked DNA markers (Collard and Mackill, 2008).…”