Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

Yara, Asanori; Phi, Cong Nguyen; Matsumura, Masaya; Yoshimura, Atsushi; Yasui, Hirofumi

doi:10.1270/jsbbs.60.639

Cited by 45 publications

(27 citation statements)

References 23 publications

(26 reference statements)

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“…Cultivating insect-resistant rice cultivars is the most effective and economic means of controlling BPH. Since the first BPH resistance gene, Bph1, was identified from the rice cultivar Mudgo, 26 resistance genes and several quantitative trait loci (QTLs) have been identified from cultivated and wild rice species (Athwal et al 1971;Alam and Cohen 1998;Jairin et al 2007aJairin et al , 2010Yara et al 2010;Jena and Kim 2010). Four main biotypes of BPH have been reported based on their ability to infect and feed on rice cultivars.…”

Section: Introductionmentioning

confidence: 99%

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Wang

et al. 2011

Planta

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Rathu Heenati (RHT) is a Sri Lankan rice cultivar that carries a brown planthopper (BPH) resistance gene, Bph3, and shows broad-spectrum resistance to all four biotypes of BPH. The BPH-resistance loci in RHT has been studied extensively and assigned to four different rice chromosomes (3, 4, 6, and 10) by different research groups, but the gene has not been cloned previously. An Affymetrix rice genome array containing 48,564 japonica and 1,260 indica sequences was used to analyze the potential resistance-related genes on the four chromosomes by comparative analysis of the differentially expressed genes between resistant and susceptible rice cultivars exposed to BPH attack. The microarray results showed that at least 17 genes related to induced resistance and at least 193 genes related to constitutive resistance in RHT. On chromosome 3, the AOC4 was hypothesized to be the most important candidate gene. On chromosome 6, no valuable candidate resistance gene was identified in the Bph3 localization region. In the three Quantitative trait locus regions of chromosomes 3, 4, and 10, the numbers of constitutive and induced resistance-related genes found were 17, 26, and 12, respectively. The major probe on chromosome 10 represents a constitutive expression gene with a very high absolute fold-change of 2,588.82. The microarray analysis indicated that BPH resistance in RHT is probably controlled by a series of resistance-related genes. This study provides valuable information for cloning, functional analysis and marker-assisted breeding of these BPH resistance genes.

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Section: Introductionmentioning

confidence: 99%

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Wang

et al. 2011

Planta

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“…Based on the reported markers and the Nipponbare or 93-11 genome sequences, Qbph-8 is different to the QTL detected by Alam and Cohen (1998) and should be located to its north. It has been noted that many BPH resistance genes appear to be clustered on rice chromosomes (Jena and Kim, 2010;Qiu et al, 2010;Yara et al, 2010). For example, seven BPH resistance genes [Bph1,bph2,Bph9,Bph10,Bph18(t),bph19(t), and Bph21(t)] cluster in one region between markers G2140 and RM5479 on chromosome 12 based on the sequence of Nipponbare.…”

Section: Discussionmentioning

confidence: 99%

Identification of a new locus conferring antixenosis to the brown planthopper in rice cultivar Swarnalata (Oryza sativa L.)

Qiu¹,

Ling²,

Liu³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The brown planthopper [Nilaparvata lugens (Stål); BPH] has caused severe damage to rice production. The identification of resistance genes and the development of BPH-resistant varieties are economical and effective ways to manage this pest. Using an F 2 population from a cross between the Indica cultivars 93-11 and Swarnalata, we mapped the Qbph-8 locus to a 7.3-cM region on chromosome 8 in two tests, flanked by the markers RM339 and RM515. In this population, Qbph-8 explained 7.7 and 6.6% of the phenotypic variance of BPH preference in both tests. In the BPH host choice test, the average number of settled BPHs on the Qbph-8 plants was less than that on the 93-11 plants over the 24-to 120-h observation period. Furthermore, less BPH insects were observed on the BPH6+Qbph-8 plant compared with the BPH6 plant or Qbph-8 plant, indicating a stronger antixenotic effect shown in the gene pyramiding plants. Hence, this locus can be pyramided with other BPH resistance genes and applied to breed-resistant varieties, which possibly can improve the resistance level and durable resistance to the BPH.

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“…Namun demikian, metode ini masih perlu divalidasi dengan membandingkannya dengan teknik baku penapisan secara massal varietas diferensial pada bak benih sebelum diaplikasikan secara luas. Sebelumnya, teknik pengujian ketahanan tanaman secara individual telah diaplikasikan pada tanaman populasi persilangan generasi awal, tetapi pada stadium anakan sehingga dapat diperoleh ulangan (Jairin et al, 2010) dan pada generasi persilangan yang telah lanjut BC 3 F 6 (Yara et al, 2010).…”

Section: Teknik Evaluasi Ketahanan Tanaman Secaraunclassified

SEGREGASI KETAHANAN POPULASI F2 PADI CIHERANG/ SWARNALATA terhadap WERENG BATANG COKLAT

nLN¹,

Ahmad²

2018

J. AgroBiogen

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Brown planthopper (BPH, Nilaparvata lugens Stål) is a serious pest of rice that significantly decreasesrice yield in Indonesia. Planting resistant varieties is an easy, inexpensive, effective, environmentally friendly, and in accordance with the concept of integrated pest management, and hence breeders continuously attempt todevelop resistant varieties to this pest. The objective of this study was to determine the resistance segregation of F 2 plants derived from Ciherang/Swarnalata cross. The study was preceded by selection of candidate parents and followed by an evaluation of test method developed for individually potted and infested seedlings on artificial segregating populations.Standard seedbox mass screening technique of twelve differential and improved rice varieties consistently identified Ciherang and Swarnalata as susceptible and resistantto BPHpopulations originated from Klaten (Central Java) and Banyuwangi (East Java), respectively. Resistance evaluation of artifical F 2 and BC 1 F 2 progenies of Ciherang/Swarnalata crossusing individually potted and infested seedlings method could demonstrated that the segregation patterns of artificial progenies were in accordance with the mixture ratios of resistant and susceptible varieties, i.e. 3 : 1 or 1 : 3 and 1 : 1 assuming inheritance pattern of monohybrid dominant or recessive in F 2 and monoybrid dominant BC 1 F 2 populations, respectively. Resistance test of 125 F 2 plants derived from Ciherang/Swarnalata cross using the developed test method showed that the plants segregated into 3 : 1 ratio for resistance and susceptible, indicating that BPH resistance in the donor parent was controlled by a single major dominant gene. The resistant F 2 plants needs to be confirmed by molecular markers to ascertain the introgression of the resistance gene and tested for their resistance in advanced generation.Keywords: Segregation, rice, resistance, brown planthopper. ABSTRAKWereng batang cokelat (WBC, Nilaparvata lugens Stål) merupakan hama yang menurunkan produktivitas padi secara signifikan di Indonesia. Penanaman varietas tahan merupakan cara yang mudah, murah, efektif, ramah lingkungan, dan sesuai dengan konsep pengendalian hama terpadu sehingga pemulia berupaya mengembangkan varietas tahan WBC. Penelitian bertujuan menguji ketahanan varietas padi untuk pemilihan calon tetua persilangan, menguji metode evaluasi ketahanan terhadap WBC pada individu tanaman untuk melengkapi metode yang telah ada, dan mempelajari segregasi populasi persilangan dari tetua terpilih. Penapisan ketahanan dua belas varietas padi diferensial dan unggul menggunakan teknik skrining massal baku dalam bak benih menunjukkan bahwa Ciherang/Swarnalata secara berurutan konsisten rentan dan tahan terhadap WBC populasi Klaten (Jawa Tengah) dan Banyuwangi (Jawa Timur) sehingga dipilih sebagai tetua persilangan. Metode evaluasi ketahanan tanaman yang ditanam dan diinfestasi dengan nimfa WBC secara individual pada tiga populasi simulasi persilangan Ciherang/Swarnalata berhasil menentukan pol...

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Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

Cited by 45 publications

References 23 publications

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Microarray analysis of broad-spectrum resistance derived from an indica cultivar Rathu Heenati

Identification of a new locus conferring antixenosis to the brown planthopper in rice cultivar Swarnalata (Oryza sativa L.)

SEGREGASI KETAHANAN POPULASI F2 PADI CIHERANG/ SWARNALATA terhadap WERENG BATANG COKLAT

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