Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from<i>β</i>-Lactoglobulin Secreted by<i>Lactococcus lactis</i>

Shigemori, Suguru; Oshiro, Kazushi; Wang, Pengfei; Yamamoto, Yuroku; Wang, Yeqin; Sato, Takashi; Uyeno, Yutaka; Shimosato, Takeshi

doi:10.1155/2014/393598

Cited by 11 publications

(10 citation statements)

References 34 publications

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“…Kefir supplementation 3.6 ml/200 g decreased expression of CD26 after exposure to CPF dose 5 mg/kg but not significant. The role of kefir is to inhibit the activity of CD26 through β-lactoglobuline which is produced by Lactococcus lactis and through the ability to decrease the level of IL-2 [46,47]. In this study, the ability of kefir in decreasing expression of CD26 is correlated with the ability to decrease the levels of TNF-α and IL-β, which increased after exposure to CPF and maintain TGF-β level not to decrease.…”

Section: Discussionmentioning

confidence: 70%

The Effects of Kefir on the Inflamatory Status and Thyroid Function (Experimental Study on Wistar Rats After Exposed to Chlorpyrifos)

Rasipin

Dharmana²,

Hadisaputro³

et al. 2016

Asian J Pharm Clin Res

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Objective: Goiter is an enlarged thyroid gland remained a health problem in the agricultural areas. Chlorpyrifos (CPF) is a pesticide widely used byfarmers. Previous studies proved that CPF exposure caused thyroid dysfunction. The objective of this study was to evaluate the effects of kefir on theinflammatory status and thyroid function in male Wistar rats after exposed to CPF using biochemical and histopathological assays.Methods: Male rats were divided into 4 groups, i.e., CPF 5+kefir (5 mg/kg+3.6 ml/200 g, respectively), CPF 5 (5 mg/kg), corn oil (CO 1 ml/200 g), andnegative control (NC: Without CPF, CO, and kefir).Results: Kefir supplementation dose 3.6 ml/200 g once a day for 28 days in the rats after exposed to CPF dose 5 mg/kg once a day for 14 days, inCPF 5+kefir as compared to CPF 5: Significantly (p<0.05) decreased serum tumor necrosis factor-α (TNF-α) level; significantly (p<0.01) maintainedserum levels of tumor growth factor-β (TGF-β) and thyroid stimulating hormone (TSH) not to decrease; not significant (p>0.05) decreased the level ofinterleukin-1β, cluster of differentiation-26 expression and level of T serum; not significant (p>0.05) maintained the level of anti-thyroid peroxidasenot to decrease; and not significant (p>0.05) increased the apoptosis index. This study suggests that CPF exposure causes the inflammatory processwhich leads to thyroid dysfunction.4Conclusion: Kefir supplementation significantly decreased the level of TNF-α and maintained the levels of TGF-β and TSH not to decrease, possible toreduce the inflammatory and thyroid dysfunction processes caused by exposure to CPF in experimental animals.Keywords: Kefir, Chlorpyrifos, Inflammation, Thyroid function.

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Section: Discussionmentioning

confidence: 70%

The Effects of Kefir on the Inflamatory Status and Thyroid Function (Experimental Study on Wistar Rats After Exposed to Chlorpyrifos)

Rasipin

Dharmana²,

Hadisaputro³

et al. 2016

Asian J Pharm Clin Res

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“…The ODN sequences used in this study are shown in Table . The following antigens (AG) were used: Buckwheat (BW, Fagopyrum esculentum , lyophilized form; GREER Laboratories, Lenoir, NC, USA) (Shigemori et al ), α‐casein from bovine milk, ≥70% α S ‐casein (CAS, Sigma, St Louis, MO, USA) (Shigemori et al ), β‐lactoglobulin (BLG, Sigma) (Shigemori et al ), and albumin from chicken egg white (Ovalbumin, OVA, grade V; Sigma).…”

Section: Methodsmentioning

confidence: 99%

Development of a simple IgE‐independent anaphylactic model using buckwheat antigen and B‐type CpG oligodeoxynucleotide from Streptococcus thermophilus

Yamamoto¹,

Shigemori

Nigar³

et al. 2015

Animal Science Journal

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We developed a severe anaphylactic model in mice using buckwheat antigen and B-type CpG-oligodeoxynucleotides (CpG-ODNs) from Streptococcus thermophilus genome. In typical systemic anaphylaxis models, animals are challenged with large quantity of antigens via an intravenous (i.v.) route. Here, we showed a simple anaphylactic shock after challenge via intraperitoneal (i.p.) route. The i.p. method is simpler than i.v. administration and has a lower risk for failure. To generate this anaphylactic model, 5-week-old female BALB/c mice were first i.p. sensitized with buckwheat antigen mixed with B-type CpG-ODN. After 2 weeks, mice were challenged with antigen to induce anaphylactic shock, which was evaluated by scoring the severity symptoms and measuring serum levels of various proteins and splenic cell producing cytokines. Immunoglobulin (Ig)G2a production and interferon-γ positive cells were markedly increased in mice immunized with antigen mixed with B-type CpG-ODN, whereas serum IgE levels were decreased by B-type CpG-ODN. We also examined the effects of various ODNs (A, B and C-type CpG-ODNs) and antigens (buckwheat, α-casein, β-lactoglobulin and ovalbumin) on anaphylactic severity, and found that the combination of buckwheat and B-type CpG-ODN induced the most intense anaphylactic shock. This model is expected to contribute to the study of the prevention of anaphylactic shock.

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“…Gene expression by gmNZ9000 was induced in 2-or 50-mL scale cultures. The experimental procedures were performed according to previously described methods (Shigemori et al, 2013(Shigemori et al, , 2014. Briefly, an overnight culture of gmNZ9000 was inoculated at 1/20 dilution into fresh GM17cm broth and incubated at 30°C until the culture reached an optical density at 600 nm (OD 600 ) of 0.4.…”

Section: Induction Of Gene Expressionmentioning

confidence: 99%

Genetically modified Lactococcus lactis producing a green fluorescent protein–bovine lactoferrin fusion protein suppresses proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 cells

Shigemori

Namai

Yamamoto

et al. 2017

Journal of Dairy Science

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Lactoferrin (LF), an iron-binding glycoprotein distributed widely in the biological fluids of mammals, is believed to play an important role in host defenses against infection. Previous studies in animal models and humans demonstrated that combined administration of LF and probiotic lactic acid bacteria (LAB) can prevent sepsis. In this study, we genetically engineered a probiotic LAB strain, Lactococcus lactis, to produce recombinant bovine LF based on the green fluorescent protein (GFP)-fused expression system. Western blotting confirmed that the genetically modified L. lactis strain (designated NZ-GFP-bLF) produced a protein corresponding to a fusion of GFP and bLF in the presence of nisin, an inducer of target gene expression. The protein synthesized by NZ-GFP-bLF was fluorescent and thus we monitored the time-dependent change in the production level of the recombinant protein using fluorometric analysis. The utility of NZ-GFP-bLF in preventing sepsis was determined by investigating its anti-inflammatory property in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells. Pretreatment of RAW 264.7 cells with NZ-GFP-bLF significantly attenuated the LPS-induced mRNA expression and protein production of 3 proinflammatory cytokines (IL-1α, IL-6, and tumor necrosis factor-α) compared with pretreatment with a vector control strain of L. lactis. Our results suggest that NZ-GFP-bLF holds promise for the development of a new prophylaxis for sepsis.

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Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides fromβ-Lactoglobulin Secreted byLactococcus lactis

Cited by 11 publications

References 34 publications

The Effects of Kefir on the Inflamatory Status and Thyroid Function (Experimental Study on Wistar Rats After Exposed to Chlorpyrifos)

The Effects of Kefir on the Inflamatory Status and Thyroid Function (Experimental Study on Wistar Rats After Exposed to Chlorpyrifos)

Development of a simple IgE‐independent anaphylactic model using buckwheat antigen and B‐type CpG oligodeoxynucleotide from Streptococcus thermophilus

Genetically modified Lactococcus lactis producing a green fluorescent protein–bovine lactoferrin fusion protein suppresses proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 cells

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